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Preparation method of polydopamine gelatin microspheres

A technology of polydopamine gelatin and dopamine, which is applied in the field of biomedicine, can solve problems such as rare research reports, and achieve the effect of smooth and transparent sphere surface, high spheroid formation rate and excellent performance

Inactive Publication Date: 2018-08-28
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, dopamine has been widely used in the surface modification of solid materials due to its strong adhesion properties, but there are few reports on the polymerization of dopamine on the surface of gelatin microspheres in order to strongly bind functional molecules

Method used

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  • Preparation method of polydopamine gelatin microspheres
  • Preparation method of polydopamine gelatin microspheres
  • Preparation method of polydopamine gelatin microspheres

Examples

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Embodiment 1

[0049] 1. Prepare gelatin microspheres by emulsification-crosslinking method, which adds a certain amount of gelatin solution as the water phase to the pre-configured liquid paraffin mixed with span-80. After emulsification for a certain period of time, turn to ice water bath. Add glutaraldehyde to cross-link and solidify, then add a certain amount of isopropanol, let stand overnight, centrifuge the next day, add isopropanol to wash several times, filter with suction, and dry. The specific operation steps are as follows:

[0050] (1). Weigh 0.3g of gelatin, dissolve it in 10mL of distilled water, and stir at 50°C until it becomes a solution.

[0051] (2). Measure 30 mL of liquid paraffin, add 0.5 mL of span-80, and stir at 50 ° C for 10 min.

[0052] (3). Add the gelatin solution dropwise into liquid paraffin and emulsify for 20 minutes.

[0053] (4). Quickly switch to ice-water bath and stir for 1 hour, add 200 μL of 2.5% glutaraldehyde, and cross-link for 1 hour.

[0054]...

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Abstract

The invention relates to a preparation method of polydopamine gelatin microspheres. The preparation method is characterized by comprising the following steps: placing prepared gelatin microspheres into a prepared Tris / HCI buffer solution of which the pH is equal to 8.5; then, adding 2mg / mL of dopamine solution; stirring at the temperature of 37 DEG C for 12 to 24 hours; centrifuging and washing with deionized water three times; drying in the air to obtain the polydopamine-coated gelatin microspheres. The gelatin microspheres are covered well with polydopamine, so that the chemical multifunctionality of the surfaces of the polydopamine gelatin microspheres is enhanced, and Vc can be loaded successfully. The polydopamine gelatin microspheres have a potential value on the aspect of drug sustained release.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to micro-nano materials, in particular to a method for preparing polydopamine gelatin microspheres. Background technique [0002] In the 1980s, American scholars Joseph P.Vacanti and Robert Langer first proposed the concept of "tissue engineering", and published their research results in the American "Science" magazine. Subsequently, the term tissue engineering was proposed by the famous Chinese-American scientist Professor Y.C. Fung and was confirmed by the National Science Foundation of the United States in 1987. In general, we define tissue engineering as mainly including three aspects: seed cells, biomaterials, and growth factors. Among them, the most important and also the focus of research is biomaterials, which is what we often call "stents". A high-quality biomaterial that can be used in actual production needs to have the following characteristics: 1. Biocompatibility and tissue c...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/18A61L27/50A61L27/54A61L27/58
CPCA61L27/222A61L27/18A61L27/50A61L27/54A61L27/58A61L2300/604A61L2300/802C08L79/02
Inventor 袁卉华李碧云王兆慧曹军牛娜昕
Owner NANTONG UNIVERSITY
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