Preparation method of phellinus linteus polysaccharides

A technology of Phellinus linteus and polysaccharide, applied in the field of extraction of natural products, can solve the problems of fluctuation of polysaccharide extraction yield, unstable dissolved quality, strict reaction conditions, etc., and achieve the effects of improving medicinal value, easy operation and improving purity.

Inactive Publication Date: 2018-08-31
许昌元化生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the operation of the ultrasonic-assisted compound enzyme method is cumbersome, and the strong shear force of the ultrasonic wave may destroy the structure of the polysaccharide, and the enzyme...

Method used

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  • Preparation method of phellinus linteus polysaccharides

Examples

Experimental program
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Embodiment 1

[0024] A preparation method of Phellinus polysaccharide, comprising the following steps:

[0025] (1) Get 1 kg of wild Phellinus japonica to remove impurities and pulverize it to a particle size of 80 orders with a universal pulverizer;

[0026] (2) freezing the Phellinus Phellinus crushed in the above step (1) at -25°C to obtain a freeze-dried powder;

[0027] (3) The freeze-dried powder obtained in step (2) is mechanically broken at -15°C, and the particle diameter after breaking is 20 μm;

[0028] (4) Adding water to the Phellinus Phellinus powder treated by the above step (3) for leaching, the mass ratio of Phellinus Phellinus powder and water is 1:30, the extraction process is leaching at 50°C for 40min, and leaching at 70°C for 90min , leached at 90°C for 70 minutes, filtered and then took the filtrate, concentrated under reduced pressure to obtain the water extract;

[0029] (5) Add 3 times the volume of ethanol, n-butanol and methanol to the water extract in the abov...

Embodiment 2

[0032] A preparation method of Phellinus polysaccharide, comprising the following steps:

[0033] (1) Get 1 kg of wild Phellinus japonica to remove impurities and pulverize it to a particle size of 90 orders with a universal pulverizer;

[0034] (2) freezing the Phellinus Phellinus crushed in the above step (1) at -20°C to obtain a freeze-dried powder;

[0035] (3) The freeze-dried powder obtained in step (2) is mechanically broken at -12°C, and the particle diameter after breaking is 25 μm;

[0036] (4) Add water to the phellinus powder treated in the above step (3) for leaching, the mass ratio of phellinus powder and water is 1:35, the extraction process is leaching at 55°C for 35min, and leaching at 75°C for 80min , leached at 95°C for 65 minutes, filtered and then took the filtrate, concentrated under reduced pressure to obtain the water extract;

[0037] (5) Add 4 times the volume of ethanol, n-butanol and methanol mixture to the water extract in the above step (4) for ...

Embodiment 3

[0040] A preparation method of Phellinus polysaccharide, comprising the following steps:

[0041] (1) Get 1kg of wild Phellinus japonica to remove impurities and pulverize it to a particle size of 100 orders with a universal pulverizer;

[0042] (2) freezing the Phellinus Phellinus pulverized in the above step (1) at -18°C to obtain a freeze-dried powder;

[0043] (3) The freeze-dried powder obtained in step (2) is mechanically broken at -10°C, and the particle diameter after breaking is 30 μm;

[0044] (4) Add water to the phellinus powder treated by the above step (3) for leaching, the mass ratio of phellinus powder and water is 1:40, the extraction process is leaching at 60°C for 30min, and leaching at 80°C for 70min , leached at 100°C for 60 minutes, filtered and then took the filtrate, concentrated under reduced pressure to obtain the water extract;

[0045] (5) Add 5 times the volume of ethanol, n-butanol and methanol to the water extract in the above step (4) to preci...

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Abstract

The invention discloses a preparation method of phellinus linteus polysaccharides. The preparation method comprises the following steps: (1) removing impurities from wild phellinus linteus and crushing the wild phellinus linteus; (2) freezing phellinus linteus in the step (1) to obtain freeze-dried powder; (3) breaking the wall of the freeze-dried powder obtained in step (2) in a low temperature environment to obtain phellinus linteus powder; (4) adding water to the phellinus linteus powder treated in the step (3) for leaching, taking a filtrate, and performing concentration under reduced pressure to obtain a water extract; (5) adding a mixture of ethanol, n-butanol and methanol to the water extract of the step (4) to obtain a precipitate; (6) washing the precipitate with an organic solvent and drying the precipitate to obtain the phellinus linteus polysaccharides. The preparation method of the phellinus linteus polysaccharides has the advantages that on the premise of maintaining activity of the phellinus linteus polysaccharides, the content and purity of the phellinus linteus polysaccharides are increased, and the medicinal value of the phellinus linteus polysaccharides is further improved.

Description

technical field [0001] The invention relates to an extraction method of natural products, in particular to an extraction method of Phellinus polysaccharide. Background technique [0002] Phellinus polysaccharide is an active substance existing in Phellinus chinensis, and it is a helical three-dimensional structure. Therefore, the current technology for extracting and purifying Phellinus polysaccharide is water extraction and alcohol precipitation method, that is, using a large amount of water to boil and reflux The raw material of Phellinus Phellinus was cooked for a long time to dissolve Phellinus polysaccharides from the cells, then add ethanol to precipitate the polysaccharides, and then filter to obtain Phellinus polysaccharides. Although Phellinus polysaccharides can be extracted from Phellinus polysaccharides by high temperature and high pressure treatment, the dissolution rate of Phellinus polysaccharides can be improved, and the recovery rate of polysaccharides can b...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张建国陈清轩姬茹石占伟刘颖杰张珂嘉臧建峰
Owner 许昌元化生物科技有限公司
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