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Preparation method and application of tripterine nanomedicine coated with hyaluronic acid

A technology of tripterine and hyaluronic acid, which is applied in the field of preparation of tripterine nano-drugs, can solve the limitations of the wide application of nano-drug loading technology, complex carrier synthesis process and drug-loading steps, and nano-carrier-assisted drug delivery system There are no problems such as therapeutic effects, and the effects of high-efficiency tumor killing, targeting, and simple and easy preparation methods are achieved

Inactive Publication Date: 2020-10-09
UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In summary, the problems in the prior art are: The nanocarrier-assisted drug delivery system has no direct therapeutic effect, and the carrier will cause additional long-term or short-term toxicity to the organism; in addition, the complex carrier synthesis process and drug loading steps limit the wide application of nanocarrier drug loading technology

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  • Preparation method and application of tripterine nanomedicine coated with hyaluronic acid
  • Preparation method and application of tripterine nanomedicine coated with hyaluronic acid
  • Preparation method and application of tripterine nanomedicine coated with hyaluronic acid

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preparation example Construction

[0035] Such as figure 1 As shown, the preparation method of hyaluronic acid-coated tripterine nanomedicine provided by the embodiments of the present invention comprises the following steps:

[0036] S101: dissolving tripterine in an organic solvent to obtain a solution A;

[0037] S102: the hyaluronic acid is dissolved in water to obtain a solution B;

[0038] S103: inject the obtained solution A into deionized water, and stir to obtain a solution C;

[0039] S104: Add the obtained solution B into the obtained solution C and mix to obtain a solution D, and stir to obtain a nano-medicine, ie tripterine nano-medicine.

[0040] The present invention prepares nano-scale drugs through the precipitation method, which does not need to be used in combination with drug carriers, avoiding the problems of biological system toxicity and immunogenicity caused by carrier materials, and the present invention uses the precipitation method to prepare nano-medicines without nano-carriers, It...

Embodiment 1

[0065] The nano-medicine provided in this embodiment includes tripterine, the particle size of the nano-medicine is 90nm, and the hydrated particle size is 120nm.

[0066] The preparation method is as follows:

[0067] (1) 5 mg tripterine was dissolved in 1.25 mL dimethyl sulfoxide to obtain a solution A whose molar concentration of tripterine was 10 mM / L;

[0068] (2) 7.8 mg of hyaluronic acid was dissolved in 1 mL of water to obtain a solution B with a hyaluronic acid molar concentration of 10 mM / L;

[0069] (3) 100 μL of the solution A obtained in step (1) was injected into 800 μL of water for three times, injected into deionized water, and magnetically stirred at a speed of 100 rpm at 20° C. to obtain solution C;

[0070] (4) 100 μL of solution B obtained in step (2) was injected into solution C obtained in step (3) and mixed to obtain solution D, which was magnetically stirred at 100 rpm at 20° C. for 24 hours to obtain nanomedicine.

[0071] The nano-medicine provided ...

Embodiment 2

[0073] The nano-medicine provided in this embodiment includes tripterine, the particle size of the nano-medicine is 80nm, and the hydrated particle size is 114.3nm.

[0074] The preparation method is as follows:

[0075] (1) 50mg tripterine was dissolved in 1.25mL methanol to obtain solution A;

[0076] (2) 78 mg of hyaluronic acid was dissolved in 1 mL of water to obtain solution B;

[0077] (3) 10 μL of the solution A obtained in step (1) was injected into 890 μL of water for three times, injected into deionized water, and magnetically stirred at 2000 rpm at 25° C. to obtain solution C;

[0078] (4) 100 μL of solution B obtained in step (2) was injected into solution C obtained in step (3) and mixed to obtain solution D, which was magnetically stirred at 25° C. at a speed of 2000 rpm for 12 hours to obtain nanomedicine.

[0079] Carry out dynamic light scattering test (DLS) to the nano-medicine provided in this embodiment, what record is the hydrated particle size of nano-...

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Abstract

The invention belongs to the technical field of cancer drugs, and discloses a preparation method and application of a hyaluronic acid coated tripterine nano-drug. The hyaluronic acid coated tripterinenano-drug is prepared from tripterine with the molar concentration being 10 to 50mM / L and hyaluronic acid with the molar concentration being 10 to 50mM / L. According to the nano-drug provided by the invention, the dosage of the tripterine can be reduced, and meanwhile, the treatment aim of high-efficient anti-tumor effect is achieved. The preparation method of the nano-drug provided by the invention is simple and practical; no nano carrier is introduced, and a drug loading ratio of 100 percent can be reached equivalently, and the problem that a large number of injection is required due to a low drug loading ratio is solved.

Description

technical field [0001] The invention belongs to the technical field of cancer drugs, in particular to a preparation method and application of tripterine nano-medicine coated with hyaluronic acid. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Cancer is one of the most serious diseases threatening human health, which involves various genetic variations and cell abnormalities. Complexity and heterogeneity promote aggressive proliferation of cancer cells, leading to generally high morbidity and mortality in cancer patients. Unfortunately, due to the low selectivity of chemotherapy drugs, it will inevitably damage the normal cells of the human body while killing cancer cells, resulting in adverse drug reactions. In addition, some chemotherapy drugs such as paclitaxel have to use organic solvents because they are insoluble in water, but the use of organic solvents often causes some immune reactions in the body, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K31/56A61K47/36A61P35/00
CPCA61K9/5161A61K31/56A61P35/00
Inventor 张占军肖亚婷陈春英
Owner UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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