Kit and method for extracting circulating DNA in plasma

An extraction method and plasma technology, applied in the field of DNA extraction, can solve the problems of high price, long extraction time, cumbersome methods, etc., and achieve the effects of preventing dust pollution, simplifying extraction steps and operations, and prolonging extraction time.
CN108486099BActive Publication Date: 2019-02-19北京爱普拜生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京爱普拜生物技术有限公司
Publication Date
2019-02-19

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Abstract

The invention provides a kit and a method for extracting circulating DNA from plasma. The kit provided by the invention comprises a DNA one-step reaction binding buffer solution, a washing solution, an eluent, and an independent aseptic packaged circulating DNA purification extension tube used in combination with a DNA binding column. The kit and the method provided by the invention can ensure that plasma samples having different starting volumes pass through the DNA binding column at a time, can effectively avoid cross-contamination between samples while rapidly and efficiently extracting high-quality circulating DNA, and have obvious advantages in comparison with common kits and methods.
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Description

technical field

[0001] The invention relates to DNA extraction and belongs to the field of nucleic acid purification, in particular to a kit and method for simply and efficiently extracting circulating DNA from blood plasma. Background technique

[0002] Blood circulating DNA is also called blood cell-free DNA. It refers to a small length of endogenous DNA that is released into the blood by human cells, frees outside the cells and circulates in the blood. The circulating DNA secreted by cancer tumor cells into the blood is also called circulating tumor DNA ( cell-tumor nucleic acid, ctDNA), is generally believed to be derived from the active secretion of tumor cells and the passive release of apoptotic cells. The value of circulating DNA in the peripheral blood of cancer patients in the diagnosis, differential diagnosis, and prognosis evaluation of various tumors has gradually been recognized. Up to now, it has been confirmed in the research of various cancers including me...

Claims

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