Processingmethod of anaerobic solid-state fermentation tobacco stems andapplication thereof

An anaerobic solid-state fermentation and treatment method technology, which is applied in the field of anaerobic solid-state fermentation tobacco stem treatment, to achieve the effects of reducing irritation, improving conversion utilization efficiency, and reducing cellulose content

Active Publication Date: 2018-09-18
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a treatment method of anaerobic solid-state fermented tobacco stems and its application, which solves the problem of reusing tobacco stems in the prior art. The selection of tobacco stem raw materials in the present invention can be adapted to local conditions. According to the local tobacco planting conditions, select The remaining tobacco stems after tobacco leaf processing are used as the main raw material, and the raw materials do not need to be rebaked

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of processing method of anaerobic solid-state fermentation tobacco stem of the present embodiment, the steps are as follows:

[0023] Weigh 200g of raw tobacco stems (including cellulose content: 20.83%; hemicellulose content: 10.66%; lignin content: 10.91%; ash content: 0.72%; pectin content: 11.18%), add 200g of tap water, and put it into fermentation Lactobacillus seed solution (the inoculum size is 0.5×10 6 per g tobacco stalks), under anaerobic conditions, the solid-state fermentation with microorganisms at room temperature was carried out for 6 days.

[0024] Test results of tobacco stem fermentation samples: cellulose content: 17.32%; hemicellulose content: 10.12%; lignin content: 9.63%; ash content: 1.00%; pectin content: 1.37%.

[0025] Add 1700g water to 290g fermented tobacco stem sample, inoculate 200mL Bacillus coagulans seed solution and 2mL cellulase, anaerobically ferment at 50°C for 72h, and the L-lactic acid content in the fermentation broth re...

Embodiment 2

[0028] A kind of processing method of anaerobic solid-state fermentation tobacco stem of the present embodiment, the steps are as follows:

[0029] Weigh 200g of raw tobacco stems (including cellulose content: 20.83%; hemicellulose content: 10.66%; lignin content: 10.91%; ash content: 0.72%; pectin content: 11.18%), add 100g of tap water, and access to feces Streptococcus seed solution (inoculum size is 10 6 per g tobacco stalks), under anaerobic conditions, the solid-state fermentation of microorganisms at room temperature was carried out for 20 days.

[0030] Test results of fermented samples: cellulose content: 19.72%; hemicellulose content: 9.92%; lignin content: 7.63%; ash content: 1.02%; pectin content: 3.37%.

[0031] 290g of fermented tobacco stem samples were added with 1700g of water, inoculated with 200mL of Bacillus coagulans seed solution and 2mL of cellulase, and anaerobically fermented at 50°C for 72h, and the L-lactic acid content in the fermentation broth rea...

Embodiment 3

[0034] A kind of processing method of anaerobic solid-state fermentation tobacco stem of the present embodiment, the steps are as follows:

[0035] Weigh 200g of raw tobacco stems (including cellulose content: 20.83%; hemicellulose content: 10.66%; lignin content: 10.91%; ash content: 0.72%; pectin content: 12.14%), add 100g of tap water, and access to feces Streptococcus seed solution (inoculum size is 10 5 per g tobacco stalks), under anaerobic conditions, the solid-state fermentation with microorganisms at room temperature was carried out for 30 days.

[0036] Test results of fermented samples: cellulose content: 19.72%; hemicellulose content: 9.92%; lignin content: 7.63%; ash content: 1.02%; pectin content: 1.07%.

[0037] Add 2340g water to 290g fermented tobacco stem sample, inoculate 200mL Bacillus coagulans seed solution and 2mL cellulase, anaerobically ferment at 50°C for 72h, and the L-lactic acid content in the fermentation broth reaches 97g / L.

[0038] The result...

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Abstract

The invention belongs to the technical field of tobacco processing and biomass utilization conversion, and particularly relates to a processingmethod of anaerobic solid-state fermentation tobacco stems andapplication thereof. In intensified fermentation pretreatment:recovered tobacco stems are cut into segments with length of 1-5cm, 0.5-1 times of water is added,seed liquid is inoculated, andsolid-state fermentation is conducted under anaerobic and normal temperature conditions for 5-30 days to obtainfermented tobacco stems; conversion and utilization of the fermented tobacco stems: 3-6 timesof water is added to the fermented tobacco stems, seed liquid and cellulase are inoculated, and anaerobic fermentation is conducted at 45-55 DEG C for 72 h to complete the anaerobic conversion process. After enhanced bacterial solid-state anaerobic fermentation, the reduction of cell wall substances can improve the content of reducing sugars intobacco stemmaterials and reduce irritation.

Description

technical field [0001] The invention belongs to the technical field of tobacco processing and biomass utilization transformation, in particular to a treatment method and application of anaerobic solid-state fermented tobacco stems. Background technique [0002] Tobacco stem is a very important part of tobacco leaves, and its weight is about 25% of the whole tobacco leaf. The pectin content accounts for 12%-20% of the tobacco stem content. During the process of smoking cigarettes, the pectin content is high, which will cause When burning, it produces a strange smell. The water-insoluble substances in the tobacco stems are mainly cell wall substances, including pectin and cellulose. The evaluation results show that: cellulose has burning paper smell; pectin has woody smell, heavy miscellaneous gas, oral irritation, poor aftertaste and other burning and smoking disadvantages, and will also change the state of smoke; too high protein content will have burning feather smell, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B5/16A24B15/20
CPCA24B5/16A24B15/20
Inventor 杨旭宋丽丽张志平王光路张靖楠
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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