Cell fixation liquid for flow detection and use method thereof

A cell fixation and flow detection technology, applied in the preparation of test samples, measurement devices, sampling, etc., can solve the problems of short storage time and many cell debris.

Inactive Publication Date: 2018-09-18
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of short storage time and many cell fragments after the existing cells are fixed, and provide a cell fixative solution for flow detection and its use method

Method used

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  • Cell fixation liquid for flow detection and use method thereof
  • Cell fixation liquid for flow detection and use method thereof
  • Cell fixation liquid for flow detection and use method thereof

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specific Embodiment approach 1

[0015] Specific embodiment one: In this embodiment, a cell fixative solution for flow detection is mixed by paraformaldehyde, glutaraldehyde, phosphate buffer and deionized water; wherein the mass concentration of paraformaldehyde in the fixative solution is 0.3-0.5%, the mass concentration of glutaraldehyde is 0.3-0.5%, and the final concentration of phosphate buffer is 0.01-0.03M.

[0016] In this embodiment, the process of glutaraldehyde immobilizing proteins through cross-linking will be accompanied by the release of hydrogen ions, which will lower the pH value of the sample. In order to keep the pH stable at an optimal level, a sufficient amount of buffer should be added to the fixative of glutaraldehyde, therefore, corresponding phosphate buffer should be added to the fixative. Glutaraldehyde can fix DNA and RNA in tissues and cells by fixing nucleoproteins, can preserve glycogen, and can also fix lipids associated with proteins or containing amino and imino groups, and ...

specific Embodiment approach 2

[0017] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass concentration of paraformaldehyde in the stationary solution is 0.4%. Others are the same as those in Embodiment 1 or 2.

specific Embodiment approach 3

[0018] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the mass concentration of glutaraldehyde in the stationary solution is 0.4%. It is the same as the specific embodiment 1 or 2.

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Abstract

The invention relates to cell fixation liquid for flow detection and a use method thereof, and aims to solve the problem that the cell storage time is short after fixation and the cell has much debrisin the prior art. The cell fixation liquid provided by the invention is prepared by mixing paraformaldehyde, glutaraldehyde, phosphate buffer solution and deionized water; a method adopted by the invention comprises the steps of firstly, dyeing a cell, then adding fixation liquid after the dyeing is finished, standing under 4 DEG C and storing in a dark place. Compared to the traditional fixationliquid, the cell fixation liquid provided by the invention has the advantages that the fixation effect is better, the cell can be stored for a longer time after fixation, the cell morphology change is smaller after being stored for 30 days, the clustering is more obvious, the fluorescence intensity has no obvious change, the cell disruption is less, the toxicity is lower and the harm to a human body is smaller. The fixation method provided by the invention has greater advantages than the traditional fixation method, and the expression rate of the cell fixed by the method provided by the invention is more accurate. The cell fixation liquid can be applied to the field of flow detection.

Description

technical field [0001] The invention relates to a cell fixative solution for flow detection and its application method. Background technique [0002] When the laboratory is doing cell flow cytometry, the cells are usually directly tested on the machine after staining to avoid long-term storage. Natural storage for too long will lead to cell fragmentation and antibody fluorescence quenching, resulting in increased cell debris, inaccurate data, and decreased fluorescence intensity. However, when there are too many samples in the laboratory or there is no flow cytometer, and it needs to be sent to a third-party laboratory, it is inevitable to store the samples for a long time. When the sample size is too small, frequent power-on and power-off will also cause a waste of instrument reagents. Generally, the samples will be collected for unified testing. At this time, the samples also need to be placed for a period of time. The traditional method adopts the fixation method, addin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N1/28
CPCG01N1/30G01N15/14G01N2001/305
Inventor 赵新新张怡郭晶刘艳青王琳娜
Owner 天晴干细胞股份有限公司
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