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A method suitable for the determination of water-soluble sugars in tobacco gene editing materials

A technology of gene editing and determination method, applied in the field of analytical chemistry, to achieve the effect of simplifying the operation of sample pretreatment

Active Publication Date: 2020-07-17
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To conduct rapid chemical analysis and evaluation of these massive materials, the existing industry standards are difficult to meet the needs of rapid screening and chemical analysis of these massive materials

Method used

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  • A method suitable for the determination of water-soluble sugars in tobacco gene editing materials
  • A method suitable for the determination of water-soluble sugars in tobacco gene editing materials
  • A method suitable for the determination of water-soluble sugars in tobacco gene editing materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The fresh tobacco leaves of 5 material samples from the gene editing breeding factory were selected as the investigation object, including the following steps:

[0038]①Sample pretreatment: Select fresh tobacco leaves from 5 material samples from the gene editing breeding factory as the investigation object, freeze-dry at -80°C, pass through a 40-mesh sieve after grinding, and accurately weigh 0.1g of the sample powder into the outer tube , add 50mL of water as the extraction agent, and then fix the inner tube of the extraction bottle on the mouth of the outer tube with a sealing ring. Press the inner sleeve of the extraction bottle, at this time the extraction solution enters the catheter through the filter sieve plate, and flows out from the catheter to achieve the effect of filtering the sample. Collect 1-1.5mL of the extract from the bend of the catheter to the outlet, and perform on-line purified ion chromatography analysis.

[0039] ②Ion chromatography analysis: ...

Embodiment 2

[0044] Take 5 kinds of finished tobacco leaves (see table 2) as investigation sample, measure carbohydrate content wherein, comprise the steps:

[0045] ① Sample pretreatment: Select 5 finished tobacco leaves currently in use as the investigation object, dry them in an oven at 40°C, then crush them and pass through a 40-mesh sieve; accurately weigh 0.1g of the sample powder into the outer tube, add 50mL of water for extraction Then, fix the inner sleeve of the extraction bottle on the mouth of the outer sleeve with a sealing ring. After installation, put it in and heat it at 50°C for 20 minutes. After the extraction is completed, press down the inner sleeve of the extraction bottle At this time, the extraction solution enters the catheter through the filter sieve and flows out from the catheter to achieve the effect of filtering the sample. Collect 1-1.5mL of the extract from the bend of the catheter to the outlet, and perform on-line purified ion chromatography analysis.

[...

Embodiment 3

[0052] Take 5 kinds of finished tobacco leaves of Baijia tobacco as investigation samples, and measure the content of carbohydrates in them. The measurement steps are the same as in Example 1. The glucose content in the samples is between 1.28-2.84wt%, and the fructose content is between 1.51-3.67wt%. , the sucrose content is between 0.57-1.68wt%.

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Abstract

The invention discloses a measurement method suitable for tobacco gene editing material water-soluble sugar. The method uses a sample extraction bottle comprising an outer sleeve (1), an inner sleeve(2), a liquid guiding tube (4) and sieve plates (3) located in an opening of the lower end of the inner sleeve (2), the number of the sieve plates is one or more; and the method comprises the following steps of (1) tobacco leaf extraction; (2) filtration and transfer; (3) online chromatogram analysis ; and (4) comparison of a peak area and a standard curve of a standard chromatogram, then the content of the tobacco gene editing material water-soluble sugar can be obtained. The method firstly adds an online solid phase extraction purification column in front of an ion chromatography analyticalcolumn and has the advantages of simple and convenient treatment and good effect; the measurement method has the advantages of quantitative accuracy, good repeatability, low detection limit and the like; and the high-throughput analysis measurement of the tobacco gene editing material water-soluble sugar is effectively achieved.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for determining water-soluble sugars suitable for tobacco gene editing materials. Background technique [0002] Sugar compounds are important components in tobacco, which have an important impact on the growth and development of tobacco and the internal quality of finished tobacco leaves. Tobacco samples contain a variety of sugars, but glucose, fructose and sucrose account for more than 90% of the total water-soluble sugar content in tobacco; therefore, the determination of glucose, fructose and sucrose in tobacco is important for understanding the growth and development of tobacco and evaluating the quality of finished tobacco leaves are of great significance. At present, there are many reports on the determination of water-soluble sugar in tobacco. The main methods include titration, near-infrared spectroscopy, continuous flow analysis, capillary electro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96
CPCG01N30/96
Inventor 李晶李雪梅刘欣向海英王晋曾婉俐孔维松杨光宇张承明许永黄海涛
Owner CHINA TOBACCO YUNNAN IND