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Preparing method for composite probiotic product

A technology of compound probiotics and probiotics, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc., can solve problems such as inactivation, and achieve the effect of storage resistance and high activity

Inactive Publication Date: 2018-09-28
GUANGDONG ZHENGDANGNIAN BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, probiotic products are active bacteria, which are very sensitive to factors such as temperature, oxygen atmosphere, humidity, and light, and are easily affected and inactivated during production, packaging, and transportation.

Method used

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  • Preparing method for composite probiotic product

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Effect test

preparation example Construction

[0023] The preparation method of the composite probiotic product provided by the invention, the method comprises the following steps:

[0024] 1) Preparation of culture medium: mixing whey powder, tryptone casein, peptone, yeast extract, inorganic salts and amino acids to prepare a liquid culture medium;

[0025] 2) Activation: Inoculate the probiotics into the liquid medium and culture them at 35-45°C to obtain an activated strain solution; the probiotics are two or more of Bifidobacterium adolescentis, Streptococcus thermophilus and Lactobacillus fermentum;

[0026] 3) Embedding: add sterilized 1-4wt% sodium alginate solution to the activated strain solution, and add dropwise to sterilized 0.05-0.2mol / L CaCl 2 In the solution, after solidification, filter to obtain embedded carrier particles;

[0027] The embedding treatment can effectively cultivate and amplify active bacteria in a directional way between activation and expansion culture, which increases the controllabilit...

Embodiment 1

[0032] Embodiment 1: the preparation of liquid culture medium

[0033] Take 5g whey powder, 5g tryptone casein, 3g peptone, 8g yeast extract, 0.1g CaCl 2 2H 2 O, 0.3g MgSO 4 ·7H 2 O, 0.8g K 2 HPO 4 , 0.8g KH 2 PO 4 , 5g NaHCO 3 , 1g NaCl, 0.3g cysteine ​​hydrochloride·H 2 After adding water and mixing evenly, adjust the pH to 7.0, add water to make the volume to 1L, and obtain a liquid medium.

Embodiment 2

[0035] A suspension of compound probiotics, made through the following steps:

[0036] 1) Activation: take 0.1 mL each of Bifidobacterium adolescentis and Lactobacillus fermentum, inoculate the inoculum into 20 mL of liquid medium, and culture at 37.5°C for 18 hours to obtain an activated strain solution;

[0037] 2) Embedding: Add 20 mL of sterilized 2.5 wt% sodium alginate solution to the activated strain solution dropwise to a sufficient amount of sterilized 0.1 mol / L CaCl 2 solution, until the amount of precipitation no longer increases, let it stand for 45 minutes, and filter to obtain embedded carrier particles;

[0038] 3) Expansion of growth: add CaCO to the liquid medium in an amount of 2.5g / 100mL 3 , inoculating the carrier particles into the liquid culture medium in an amount of 12 g / mL to obtain probiotic particles, and washing with sterile water;

[0039] 4) Suspension: Add probiotic granules to 20 mL of 2.5 wt% sodium citrate solution, and place at 37.5° C. for...

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Abstract

The invention discloses a preparing method for a composite probiotic product. The preparation method comprises the steps that firstly, active strains with the high concentration are obtained through activating culture; carrier particles are formed through embedding; then, amplification culture is conducted, and probiotics grow and reproduce in the carrier particles; and finally, the probiotics arereleased from the carrier particles through suspension, and thus probiotic suspension liquid with the density reaching 10<11> cfu / mL or above can be obtained. The probiotic suspension liquid can be directly subjected to freeze drying or the probiotic suspension liquid and accessories including jerusalem artichoke raw powder can be further subjected to agent preparation. According to the preparation method, it can be ensured that the probiotic suspension liquid has the high strain purity, concentration and operability, and the obtained strain product is high in activity and resistant to store,and has good industrial application prospects.

Description

technical field [0001] The invention belongs to the field of health food / drug technology, and in particular relates to a composite probiotic product and a preparation method thereof. Background technique [0002] Probiotics have high application value in important fields closely related to human life, such as industry, agriculture, medicine, food, and feed. The research on the use of probiotics in health food and health medicines started relatively late in China, and it was only in the 1990s that probiotics were used in various health foods. [0003] However, probiotic products are active bacteria, which are very sensitive to factors such as temperature, oxygen atmosphere, humidity, and light, and are easily affected and inactivated during production, packaging, and transportation. This has strict hygiene and safety standards for the probiotics product industry. Contents of the invention [0004] In order to overcome the deficiencies of the prior art, the object of the p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23P10/30C12R1/01C12R1/46C12R1/225
CPCA23L33/135A23P10/30C12N1/20A23V2002/00A23V2400/143A23V2400/513A23V2400/249A23V2200/224A23V2250/5026A23V2250/5062A23V2250/51086A23V2250/6418
Inventor 陆新军
Owner GUANGDONG ZHENGDANGNIAN BIO TECH CO LTD
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