Method for mass production of tissue chip

A technology of tissue chip and production method, which is applied in the direction of measuring device, test sample preparation, sampling, etc., can solve the problems of limited tissue thickness, inability to achieve mass production, and difficulty in providing tissue structure information, etc., to achieve diverse shaping Effect

Active Publication Date: 2018-09-28
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the preparation of tissue chips according to the above method has the following disadvantages: first, the production cost of existing tissue chips is very high
In addition to special equipment (hundreds of thousands of yuan), the cost of making a tissue chip is more than 10,000 yuan, and the unit price of each slice is 50-100 yuan; The source is limited; thirdly, according to the existing equipment and methods, the chip points are lost after only about 50 slices are made, because the samples are sampled on the original wax block, and the tissue thickness is limited and the tissue thickness is not consistent; Fourth, although the high-density chip has a large throughput, it is difficult to provide tissue structure information due to the small size of each tissue; fifth, only paraffin tissue can be used to prepare tissue chips, and fresh tissues and cultured cells cannot be used to prepare tissue chips; Sixth, only large-scale research units have the funds and conditions to conduct related research on tissue chips, which is difficult to promote
Seventh, the existing tissue chip method cannot achieve mass production, and it is difficult to meet the needs of large-scale clinical and scientific research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A tissue chip mass production method, the specific steps are:

[0030] Step 1: Take the required tissues when collecting pathological materials, fix them with formalin, number them, and crush them with a tissue mixer to form tissue pellets, collect the tissue pellets, wrap them in cotton cloth, and follow the routine tissue processing procedures of the pathology department, after numbering Dehydration soaking wax;

[0031] Step 2: Turn on the tissue microarray embedding machine, place the tissue pellets on the ironing table of the tissue microarray embedding machine, melt the paraffin on the tissue microarrays at 60°C, that is the tissue pellets to be shaped, put the shaping tube Put it into the groove on the ironing table of the tissue chip embedding machine. The shaping tube is a cylindrical plastic tube with a length of 2 cm and a diameter of 0.3 cm. Insert the tissue pellets to be shaped into the shaping tube, add a little melted Paraffin wax to fill the gaps betwe...

Embodiment 2

[0036] A tissue chip mass production method, the specific steps are:

[0037] Step 1: When collecting pathological materials, take the required fresh tissues, number them, and use a "tissue blender" to stir them into tissue pellets;

[0038] Step 2: Use a straw to move the tissue granules into the groove of the fresh tissue granule shaping plate. The fresh tissue granule shaping plate is an elastic plastic plate with a groove on the plastic plate. The groove is 2cm long and 0.3cm wide. 0.3cm deep, place the fresh tissue pellet shaping plate in a -20°C refrigerator, and freeze and shape the tissue pellet into a fresh tissue cryopreservation column;

[0039] Step 3: Use the elasticity of the fresh tissue granule shaping plate to loosen the shaped fresh tissue cores, take them out with tweezers, number them, and freeze the fresh tissue cores for later use.

[0040] Step 4: Place a 50mm×25mm metal plate in a -20°C refrigerator with positioning points marked on the metal plate, ta...

Embodiment 3

[0042] A tissue chip mass production method, the specific steps are:

[0043] Step 1: The exfoliated cells of the pleural effusion were obtained by centrifugation, fixed in formalin by conventional methods, wrapped in cotton cloth, followed by routine tissue processing procedures in the pathology department, dehydrated and soaked in wax;

[0044] Step 2: Put the wax-soaked exfoliated cells on the ironing table of the tissue chip embedding machine, melt the paraffin wax on the tissue pellet at 62°C, and put the shaping tube into the groove on the ironing table of the tissue chip embedding machine Among them, the shaping tube is a cuboid shaped tube with a length of 2 cm, a width of 0.2 cm, and a height of 0.2 cm. The cells soaked in wax are placed in the shaping tube, and melted paraffin is added to fill the gap; Quantity, prepare 10 cell chips at a time;

[0045] Step 3: Transfer the shaping tube from the ironing table to the freezing table of the tissue chip embedding machin...

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Abstract

The invention provides a method for mass production of a tissue or cell chip. The method comprises shaping tissue particles, cultured cells or collected exfoliated cells (such as pleuroperitoneal fluid exfoliated cells) through a shaping device. The tissue particles are obtained through smashing fresh tissue, tissue fixed by formalin or other solvents (such as alcohol), or paraffin-embedded tissueblocks through a tissue blender. The shaped tissue or cells are implanted into a receptor wax mold or are bonded to a metal plate and then are sliced so that a tissue or cell chip is obtained. The method has the advantages of low cost, convenience, rapidity, mass production and variety of shapes, can be used for exfoliated cells of fresh tissue, fixed tissue, paraffin-embedded tissue and cultureor pleuroperitoneal fluid, and can be spread to every pathology and related research unit.

Description

technical field [0001] The invention relates to a mass production method for making tissue chips, which is used for preparing super-high-yield tissue chips and belongs to the technical field of tissue chips. Background technique [0002] Tissue chip technology is the development and extension of gene chip technology. Like cell chip, protein chip and antibody chip, it belongs to a special biochip technology. Tissue chip technology can arrange dozens or even thousands of clinical tissue specimens of different individuals on a slide for analysis and research in a pre-designed order. It is a high-throughput, multi-sample analysis tool. Tissue chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can be combined with DNA, RNA, protein, antibody and other technologies, combined with traditional pathology technology, histo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N1/286G01N2001/2873G01N1/36G01N1/06G01N1/42G01N2001/2866G01N2001/364
Inventor 侯英勇蒋冬先徐晨宿杰阿克苏陈伶俐刘亚岚王海星黄洁宋琦刘佳曾海英徐一凡纪元卢韶华侯君罗荣奎栾丽娟
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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