Method for mass production of tissue chip

Abstract

The invention provides a method for mass production of a tissue or cell chip. The method comprises shaping tissue particles, cultured cells or collected exfoliated cells (such as pleuroperitoneal fluid exfoliated cells) through a shaping device. The tissue particles are obtained through smashing fresh tissue, tissue fixed by formalin or other solvents (such as alcohol), or paraffin-embedded tissueblocks through a tissue blender. The shaped tissue or cells are implanted into a receptor wax mold or are bonded to a metal plate and then are sliced so that a tissue or cell chip is obtained. The method has the advantages of low cost, convenience, rapidity, mass production and variety of shapes, can be used for exfoliated cells of fresh tissue, fixed tissue, paraffin-embedded tissue and cultureor pleuroperitoneal fluid, and can be spread to every pathology and related research unit.

Description

technical field

[0001] The invention relates to a mass production method for making tissue chips, which is used for preparing super-high-yield tissue chips and belongs to the technical field of tissue chips. Background technique

[0002] Tissue chip technology is the development and extension of gene chip technology. Like cell chip, protein chip and antibody chip, it belongs to a special biochip technology. Tissue chip technology can arrange dozens or even thousands of clinical tissue specimens of different individuals on a slide for analysis and research in a pre-designed order. It is a high-throughput, multi-sample analysis tool. Tissue chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can be combined with DNA, RNA, protein, antibody and other technologies, combined with traditional pathology technology, histo...

Examples

Embodiment 1

[0029] A tissue chip mass production method, the specific steps are:

[0030] Step 1: Take the required tissues when collecting pathological materials, fix them with formalin, number them, and crush them with a tissue mixer to form tissue pellets, collect the tissue pellets, wrap them in cotton cloth, and follow the routine tissue processing procedures of the pathology department, after numbering Dehydration soaking wax;

[0031] Step 2: Turn on the tissue microarray embedding machine, place the tissue pellets on the ironing table of the tissue microarray embedding machine, melt the paraffin on the tissue microarrays at 60°C, that is the tissue pellets to be shaped, put the shaping tube Put it into the groove on the ironing table of the tissue chip embedding machine. The shaping tube is a cylindrical plastic tube with a length of 2 cm and a diameter of 0.3 cm. Insert the tissue pellets to be shaped into the shaping tube, add a little melted Paraffin wax to fill the gaps betwe...

Embodiment 2

[0036] A tissue chip mass production method, the specific steps are:

[0037] Step 1: When collecting pathological materials, take the required fresh tissues, number them, and use a "tissue blender" to stir them into tissue pellets;

[0038] Step 2: Use a straw to move the tissue granules into the groove of the fresh tissue granule shaping plate. The fresh tissue granule shaping plate is an elastic plastic plate with a groove on the plastic plate. The groove is 2cm long and 0.3cm wide. 0.3cm deep, place the fresh tissue pellet shaping plate in a -20°C refrigerator, and freeze and shape the tissue pellet into a fresh tissue cryopreservation column;

[0039] Step 3: Use the elasticity of the fresh tissue granule shaping plate to loosen the shaped fresh tissue cores, take them out with tweezers, number them, and freeze the fresh tissue cores for later use.

[0040] Step 4: Place a 50mm×25mm metal plate in a -20°C refrigerator with positioning points marked on the metal plate, ta...

Embodiment 3

[0042] A tissue chip mass production method, the specific steps are:

[0043] Step 1: The exfoliated cells of the pleural effusion were obtained by centrifugation, fixed in formalin by conventional methods, wrapped in cotton cloth, followed by routine tissue processing procedures in the pathology department, dehydrated and soaked in wax;

[0044] Step 2: Put the wax-soaked exfoliated cells on the ironing table of the tissue chip embedding machine, melt the paraffin wax on the tissue pellet at 62°C, and put the shaping tube into the groove on the ironing table of the tissue chip embedding machine Among them, the shaping tube is a cuboid shaped tube with a length of 2 cm, a width of 0.2 cm, and a height of 0.2 cm. The cells soaked in wax are placed in the shaping tube, and melted paraffin is added to fill the gap; Quantity, prepare 10 cell chips at a time;

[0045] Step 3: Transfer the shaping tube from the ironing table to the freezing table of the tissue chip embedding machin...

technical field

[0001] The invention relates to a mass production method for making tissue chips, which is used for preparing super-high-yield tissue chips and belongs to the technical field of tissue chips. Background technique

[0002] Tissue chip technology is the development and extension of gene chip technology. Like cell chip, protein chip and antibody chip, it belongs to a special biochip technology. Tissue chip technology can arrange dozens or even thousands of clinical tissue specimens of different individuals on a slide for analysis and research in a pre-designed order. It is a high-throughput, multi-sample analysis tool. Tissue chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can be combined with DNA, RNA, protein, antibody and other technologies, combined with traditional pathology technology, histo...

Examples

Embodiment 1

[0029] A tissue chip mass production method, the specific steps are:

[0030] Step 1: Take the required tissues when collecting pathological materials, fix them with formalin, number them, and crush them with a tissue mixer to form tissue pellets, collect the tissue pellets, wrap them in cotton cloth, and follow the routine tissue processing procedures of the pathology department, after numbering Dehydration soaking wax;

[0031] Step 2: Turn on the tissue microarray embedding machine, place the tissue pellets on the ironing table of the tissue microarray embedding machine, melt the paraffin on the tissue microarrays at 60°C, that is the tissue pellets to be shaped, put the shaping tube Put it into the groove on the ironing table of the tissue chip embedding machine. The shaping tube is a cylindrical plastic tube with a length of 2 cm and a diameter of 0.3 cm. Insert the tissue pellets to be shaped into the shaping tube, add a little melted Paraffin wax to fill the gaps betwe...

Embodiment 2

[0036] A tissue chip mass production method, the specific steps are:

[0037] Step 1: When collecting pathological materials, take the required fresh tissues, number them, and use a "tissue blender" to stir them into tissue pellets;

[0038] Step 2: Use a straw to move the tissue granules into the groove of the fresh tissue granule shaping plate. The fresh tissue granule shaping plate is an elastic plastic plate with a groove on the plastic plate. The groove is 2cm long and 0.3cm wide. 0.3cm deep, place the fresh tissue pellet shaping plate in a -20°C refrigerator, and freeze and shape the tissue pellet into a fresh tissue cryopreservation column;

[0039] Step 3: Use the elasticity of the fresh tissue granule shaping plate to loosen the shaped fresh tissue cores, take them out with tweezers, number them, and freeze the fresh tissue cores for later use.

[0040] Step 4: Place a 50mm×25mm metal plate in a -20°C refrigerator with positioning points marked on the metal plate, ta...

Embodiment 3

[0042] A tissue chip mass production method, the specific steps are:

[0043] Step 1: The exfoliated cells of the pleural effusion were obtained by centrifugation, fixed in formalin by conventional methods, wrapped in cotton cloth, followed by routine tissue processing procedures in the pathology department, dehydrated and soaked in wax;

[0044] Step 2: Put the wax-soaked exfoliated cells on the ironing table of the tissue chip embedding machine, melt the paraffin wax on the tissue pellet at 62°C, and put the shaping tube into the groove on the ironing table of the tissue chip embedding machine Among them, the shaping tube is a cuboid shaped tube with a length of 2 cm, a width of 0.2 cm, and a height of 0.2 cm. The cells soaked in wax are placed in the shaping tube, and melted paraffin is added to fill the gap; Quantity, prepare 10 cell chips at a time;

[0045] Step 3: Transfer the shaping tube from the ironing table to the freezing table of the tissue chip embedding machin...