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Method for determining content of trace metals in biological tissues and body fluids

A technology for biological tissue and trace metals, which is used in measurement devices, preparation of samples for testing, and material analysis by electromagnetic means. , the purification effect of complex sample matrix is ​​not ideal, etc., to achieve the effect of fast and accurate measurement results and easy operation

Inactive Publication Date: 2018-09-28
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the viscosity of the surfactant phase is high, and it needs to be diluted with a solvent before being tested on the machine, and this method is easy to enrich the matrix at the same time, causing detection interference and affecting the accuracy, precision and stability of the detection. digest
Other enrichment methods such as liquid-liquid extraction and solid-phase extraction are still not ideal for purification of complex sample matrices

Method used

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  • Method for determining content of trace metals in biological tissues and body fluids
  • Method for determining content of trace metals in biological tissues and body fluids
  • Method for determining content of trace metals in biological tissues and body fluids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 (detect trace metal content in blood sample)

[0023] 1. Instruments, reagents and samples

[0024] 1.1. Main instruments: inductively coupled plasma mass spectrometer (ICP-MS) (PerkinElmer NexION 350X); G13U desktop centrifuge; precision ordinary micro acidity meter (SevenCompactTMS210-K); electronic balance (BS423S); vortex mixer (Vortex -6); ultrapure water treatment system (Milli-Q Element A10System); XMTD-204 constant temperature water bath; plastic volumetric flask (50mL); centrifuge tube (2mL, 50mL); graduated cylinder (500mL); pipette.

[0025] 1.2. Main reagents: ICP-MS tuning solution (Be, Ce, Fe, In, Li, Mg, Pb, U) 1μg / L; internal standard element standard solution (Sc, Ge, Y, In, Tb) 1000mg / L ;Element standard solution: Li, Be, B, Mg, Al, Ca, Ti, V, Cr, Mn, Co, Ni, Cu, Zn, Rb, Sr, Mo, Ag, Cd, Sn, Sb, Ba, Tl, Pb, Th, U (concentrations are all 1000mg / L); Triton X-100 (purity>98%); nitric acid (GR (65%≤purity≤68%)); ultrapure water by Milli-Q Ele...

Embodiment 2

[0099] Embodiment 2 (detect trace metal content in bovine liver)

[0100] 1. Instruments, reagents and samples

[0101] 1.1. Main instruments: the same as in Example 1.

[0102] 1.2. Main reagents: the same as in Example 1.

[0103] 1.3. Solution preparation

[0104] 1.3.1. Diluent: add 5mL of nitric acid, add ultrapure water to 500mL, mix well to get 1% nitric acid diluent;

[0105] 1.3.2. Internal standard working solution: five internal standard elements scandium (45Sc), germanium (74Ge), yttrium (89Y), indium (115In) and terbium (159Tb) are prepared with diluents to a concentration of 20 μg / L internal standard working solution;

[0106] 1.3.3. TritonX-100 solution with a mass concentration of 3%: Mix Triton X-100 and a nitric acid solution with a volume concentration of 0.1% at a mass ratio of 3:97 and vortex to obtain 3% Triton X-100 solution;

[0107] 1.3.4. Standard stock solutions of mixed element series: the standard stock solutions were obtained by diluting th...

Embodiment 3

[0152] Embodiment 3 (the impact of different concentrations of Triton X-100 on blood sample processing results)

[0153] 1. Prepare a Triton X-100 solution with a mass concentration of 0.1%: Mix Triton X-100 and a nitric acid solution with a volume concentration of 0.1% in a mass ratio of 1:999, vortex and mix to obtain a mass concentration of 0.1% TritonX-100 solution;

[0154] 2. Take two 2mL EP tubes, first add 90 μL of mixed blood samples, and then add 1800 μL of Triton X-100 solution with a mass concentration of 3% and Triton X-100 solution with a mass concentration of 0.1%. Perform vortex oscillation treatment respectively; add 3% Triton X-100 solution to the EP tube, then use a water bath at 90°C for 20 minutes, centrifuge at 12000r / min for 5 minutes, and then ice-bath for 5 minutes to 0°C; observe the two EP tubes Phenomena in blood samples.

[0155] 3. The results showed that after adding the Triton X-100 solution with a mass concentration of 0.1%, the blood sample ...

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Abstract

The invention relates to a method for determining the content of trace metals in biological tissues and body fluids. The method comprises the steps: taking a uniformly-blended biological tissue sampleor body fluid, adding 20-40 volume times of TritonX-100 solution with a mass concentration of 2% to 5%, performing vortex oscillation, treating in a 60 DEG C to 90 DEG C constant temperature water bath for 15min to 50min, treating in an ice bath to 0 DEG C after centrifuging, and taking a supernatant to obtain a sample injection solution of a sample to be determined; analyzing the sample injection solution of the sample to be determined by an inductively coupled plasma mass spectrometry to obtain the content of trace metal elements in biological tissue or body fluid samples. The trace metal detection can be carried out by firstly separating a complex interference matrix in the tissue and the body fluid samples by using the 2% to 5% TritonX-100 solution, and then adopting the inductively coupled plasma mass spectrometry. The method has the advantages of simplicity and easiness in operation, and fastness and accuracy in result compared with the prior art.

Description

technical field [0001] The present invention relates to testing or analyzing materials by means of measuring their chemical or physical properties, in particular to an analysis method for measuring trace metal content in biological tissues and body fluids. Background technique [0002] The human body is composed of a variety of chemical elements. 81 elements in nature have been detected in the human body. According to their effects on the human body, they are divided into three types: essential elements, non-essential elements and toxic elements. According to the content in the human body, they can be divided into three types: Major elements and trace elements. Due to the ubiquity of elements in the human body and the environment, the content of toxic elements in the human body will not always be zero. Toxic elements combine with enzymes in the body to inactivate the original normal enzymes, thereby affecting normal metabolism. For example, Tl poisoning manifests as hair l...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N1/28
CPCG01N1/28G01N27/62
Inventor 潘加亮谭婉儿叶横明黎惠婷马安德
Owner SOUTHERN MEDICAL UNIVERSITY