Method for detecting deltamethrin in strawberry based on gold nanoparticle surface Raman enhancement

Abstract

The invention discloses a method for detecting deltamethrin in strawberries based on gold nanoparticle surface Raman enhancement. The method comprises the following steps: 1, uniformly mixing strawberries with a deltamethrin pesticide with acetonitrile and sodium chloride, and sequentially carrying out vortex oscillation and centrifugation so as to obtain supernate; 2, mixing anhydrous magnesium sulfate, propyl-ethylenediamine, C18 and graphitized carbon black with the supernate, and sequentially carrying out vortex oscillation, centrifugation and filtering so as to obtain a liquid to be tested; 3, preparing a gold nanoparticle sol substrate; 4, uniformly mixing the gold nanoparticle sol substrate, the liquid to be tested and a sodium chloride solution, and carrying out Raman spectrum acquisition; 5, according to equations, calculating residues of the deltamethrin pesticide in the strawberries. By adopting the deltamethrin pesticide residue detection method disclosed by the invention,a rapid, cheap, simple, convenient and accurate detection scheme is provided for pesticide residue detection.

Description

technical field [0001] The invention relates to the technical field of plant pesticide residue detection, in particular to a method for detecting deltamethrin in strawberries based on gold nano surface Raman enhancement. Background technique [0002] Red-faced strawberry seedlings (also known as red-cheeked strawberry, Japan No. 99, red-cheeked) are the largest strawberry series planted in the market at present. The red-faced strawberry has a strong growth potential, a tall plant (25 cm), a large plant diameter, and the ability to produce new stems Medium, large and thick leaves, light green petiole, slightly red base sheath, thick stolon, medium pumping ability, thick peduncle, one large and one small two complete leaves on the branches. Deltamethrin is a benzimidazole derivative, which is a systemic broad-spectrum fungicide. It is widely used in the prevention and treatment of red spider mites, aphids, and grubs in strawberries. Long-term contact or accidental ingestion ca...

AI Technical Summary

Problems solved by technology

Although the above research can use surface-enhanced Raman spectroscopy to achieve the purpose of enhancing the signal of pesticide molecules in agricultural products, it cannot fully remove the influence of matrix substances, the detection limit of the method needs to be further improved, and the accuracy and stability of the method are poor. Its detection method needs to be further improved. In addition, there is no report on the use of surface-enhanced Raman spectroscopy to detect deltamethrin fungicide pesticide residues in strawberries.

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