Method for inducing stem cell differentiation by utilizing bacterial cellulose piezoelectric hydrogel

A bacterial cellulose and stem cell differentiation technology, applied in animal cells, nervous system cells, vertebrate cells, etc., can solve the problems of complexity, huge system, inconvenient application, etc., and achieve good results

Active Publication Date: 2018-10-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the existing electrical stimulation methods need to rely on exte

Method used

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  • Method for inducing stem cell differentiation by utilizing bacterial cellulose piezoelectric hydrogel
  • Method for inducing stem cell differentiation by utilizing bacterial cellulose piezoelectric hydrogel
  • Method for inducing stem cell differentiation by utilizing bacterial cellulose piezoelectric hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0038] ① Treat the sheet-shaped bacterial cellulose hydrogel purchased by Guilin Qihong Technology Co., Ltd. with 1% SDS deionized aqueous solution at 30°C for 1 hour, and treat it with 1% NaOH deionized aqueous solution at 40°C for 1 hour. Purify the sheet-shaped bacterial cellulose hydrogel; after soaking and washing with deionized water, cut the purified sheet-shaped bacterial cellulose hydrogel into 0.5×0.5cm 2 A small piece of spare.

[0039] In order to observe the microscopic morphology of the sheet-like bacterial cellulose hydrogel, the sheet-like bacterial cellulose hydrogel was freeze-dried and observed with a scanning electron microscope (SEM). The photos are as follows: figure 1 As shown, the bacterial cellulose has a fibrous network structure.

[0040] In order to detect the piezoelectric response of bacterial cellulose, the freeze-dried bacterial cellulose was tested with a piezoelectric force microscope, and the obtained piezoelectric signal diagram is as follo...

Embodiment 2

[0044] ① The sheet-shaped bacterial cellulose hydrogel purchased by Guilin Qihong Technology Co., Ltd. was treated with 5.5% SDS deionized aqueous solution at 105°C for 12.5 hours, and treated with 5.5% NaOH deionized aqueous solution at 100°C for 4.5 hours. h for purification of sheet-like bacterial cellulose hydrogels. After fully soaking and washing with deionized water, cut the purified sheet-shaped bacterial cellulose hydrogel into 1.25×1.25cm 2 small pieces of spare;

[0045] ②Place the cell climbing sheet and the flake bacterial cellulose hydrogel sheet in the orifice plate respectively, add 75% alcohol and soak in it for 30 hours, and at the same time sterilize and disinfect it with ultraviolet light for 105 minutes in the aseptic operation table, and use PBS to sterilize it. Wash off the alcohol for later use;

[0046] ③Digest the stem cells cultured in the cell culture flask and disperse them with cell culture medium to obtain a stem cell suspension. The volume is ...

Embodiment 3

[0048] ① The sheet-shaped bacterial cellulose hydrogel purchased by Guilin Qihong Technology Co., Ltd. was treated with 10% SDS deionized aqueous solution at 180°C for 24 hours, and treated with 10% NaOH deionized aqueous solution at 160°C for 8 hours. Purify the sheet-shaped bacterial cellulose hydrogel; after soaking and washing with deionized water, cut the purified sheet-shaped bacterial cellulose hydrogel into 2×2cm 2 small pieces of spare;

[0049] ②Place the cell climbing sheet and the sheet-like bacterial cellulose hydrogel sheet in the orifice plate respectively, add 75% alcohol and soak in it for 48 hours, and at the same time sterilize it with ultraviolet light for 180 minutes in the aseptic operation table, and use PBS to sterilize it. Wash off the alcohol for later use;

[0050] ③Digest the stem cells cultured in the cell culture flask and then disperse them with cell culture medium to obtain a stem cell suspension. The volume is 5ml, and the number of cells cont...

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Abstract

The invention discloses a method for inducing stem cell differentiation by utilizing bacterial cellulose piezoelectric hydrogel. The method comprises the following specific steps: treating flake bacterial cellulose hydrogel with SDS deionized aqueous solution, treating with a NaOH deionized aqueous solution, and fully soaking and cleaning with deionized water; placing flake bacterial cellulose hydrogel pieces into a pore plate; digesting stem cells cultured in a cell culture bottle, dispersing to obtain stem cell suspension by using a cell culture medium, inoculating to the surface of the flake bacterial cellulose hydrogel in the pore plate, culturing for 1-90 days, and detecting the content and expression level of specific proteins and genes related to neural differentiation in cells. Theinvention first discovers and discloses a method for inducing stem cells to be differentiated to nerves or glial cells by using the bacterial cellulose piezoelectric hydrogel, and tests prove that the effect of differentiating the stem cells to the nerves or glial cells by virtue of the flake bacterial cellulose hydrogel is excellent.

Description

technical field [0001] The invention relates to a method for inducing differentiation of stem cells by using bacterial cellulose piezoelectric hydrogel, in particular to a method for inducing differentiation of stem cells to nerve or glial cells by using sheet-shaped bacterial cellulose piezoelectric hydrogel, which belongs to biological materials technology field. Background technique [0002] With the development of society, the field of tissue engineering research has been widely concerned and developed, and nerve repair and regeneration is one of the most important research contents. How to realize the rapid induction of stem cells to nerve differentiation has become one of the important contents of people's research. Existing methods for inducing neural differentiation of stem cells include using biochemical factors, induction media or biological materials to induce or promote the differentiation of stem cells into neural or glial cells. However, the induction time of ...

Claims

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Application Information

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IPC IPC(8): C12N5/079C12N5/0775
CPCC12N5/0618C12N2506/1353C12N2506/1361C12N2506/1376
Inventor 刘宏张珊马保金刘锋段佳志王世才孔颖杜敏桑元华王建军
Owner SHANDONG UNIV
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