The Method of Stem Apex Regeneration and Multiplication of Mongolian Medicine Helan Mountain Syringa

Abstract

The invention belongs to the field of plant tissue culture, and relates to a research on a method for the regeneration and propagation of the stem tip of Helan Mountain lilac, which is characterized in that: the method for the regeneration and propagation of the stem tip of Helan Mountain lilac is as follows: cut the stem section with buds from a strong mother plant, After disinfection treatment, the stem tips are stripped and put into the culture medium for primary culture; clustered buds grow, and a single clustered bud is cut off and then subcultured to obtain a large number of buds of Helan Mountain lilac, and the buds are divided into individual strains, and then carry out rooting culture, seedling hardening and transplanting to obtain transplanted seedlings of Helan Mountain lilac. The beneficial effects are: providing a method for regeneration and propagation of Helan Mountain lilac stem tips that is fast in propagation speed, high in quality, stable in maternal genetic traits, suitable for large-scale seedling breeding, simple, easy to implement, green and environmentally friendly, and easy to use and popularize, for The production of precious Mongolian medicinal materials provides a large number of high-quality seedling resources, provides product quality assurance for the unified management of industrialization, reduces the production cost of enterprises, and brings huge economic benefits.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a research on a method for regeneration and multiplication of the stem tip of the Mongolian medicine Helan Mountain syringa, in particular to the research on the plant tissue culture technique of the Mongolian medicine Helan Mountain syringa stem tip inducing clustered buds. Background technique [0002] Helan Mountain Lilac (Syringa pinnatifolia Hemsl.var.alashgnensis Maet s.Q.zhou) is a plant of the genus Clove in the Oleaceae family. It is also known as Mountain Agarwood and Mongolian name alasha goldu bori. It is a variant of the same plant Syringa pinnatifolia Hemsley. It mainly grows in Helan Mountain The shady miscellaneous woods and irrigation in the valley are between 2,000 and 3,000 meters above sea level. The roots and stems can be used as medicine. Agarwood, which can be used as medicine instead of agarwood, is a precious medicinal material in Mongolian medicine. In...

AI Technical Summary

Problems solved by technology

At present, Helan Mountain lilac is mainly propagated by sowing and cuttings, but its seed germination rate is low and the seedling cycle is relatively long, while cuttings are difficult to take root, require a large amount of female parent materials and are easy to damage the tree shape, and their reproduction rate Far from meeting the needs of its market

The use of plant tissue culture can overcome the above shortcomings, but because of different plant tissues, the degree of difficulty of culture varies greatly. The same material, due to factors such as different organ sources, culture medium, and temperature, will also lead to tissue culture. Therefore, it is particularly important to find a rapid, effective, stable genetic traits, and a method of multiplying Helan Mountain lilac that can be mass-produced