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Method for preparing Janus particles based on lysozyme nano-film

A technology of nano-film and micro-nano particles, which is applied in the field of preparation of Janus particles, achieves the effect of wide applicability, simple operation, and elimination of complicated operation

Active Publication Date: 2018-10-19
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few methods that can successfully prepare Janus particles through a simple one-step method, such as interface modification based on Pickering emulsion, self-assembly of block copolymers, microfluidic synthesis, electroco-spraying, swelling emulsion polymerization

Method used

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  • Method for preparing Janus particles based on lysozyme nano-film
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  • Method for preparing Janus particles based on lysozyme nano-film

Examples

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Effect test

Embodiment 1

[0017] 1. Adjust 10mL of 50mmol / L tris(2-carboxyethyl)phosphine in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to pH 7.0 with NaOH, and then mix it with 10mL of 2mg / mL lysozyme 4 -Hydroxyethylpiperazineethanesulfonic acid buffer solution is mixed evenly, and then the glass sheet is immersed in the resulting mixture, incubated at room temperature for 5 minutes, so that the surface of the glass sheet is adhered to a layer of lysozyme nano-film, and finally cleaned with deionized water to obtain Glass slides modified with lysozyme nanofilms.

[0018] 2. Uniformly disperse a layer of polystyrene microsphere dispersion liquid with a particle size of 10 μm on the surface of the glass sheet modified by the lysozyme nano-film, and then vacuum-dry it to obtain a glass sheet adsorbing the polystyrene microsphere.

[0019] 3. Adjust 10mL of 50mmol / L tris(2-carboxyethyl)phosphine in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to pH 7.0 with NaOH, and then m...

Embodiment 2

[0021] 1. Adjust 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of 10mL 40mmol / L tris(2-carboxyethyl)phosphine to pH 7.5 with NaOH, and then mix it with 10mL 2mg / mL lysozyme 4 -Hydroxyethylpiperazineethanesulfonic acid buffer solution is mixed evenly, and then the glass sheet is immersed in the resulting mixture, incubated at room temperature for 5 minutes, so that the surface of the glass sheet is adhered to a layer of lysozyme nano-film, and finally cleaned with deionized water to obtain Glass slides modified with lysozyme nanofilms.

[0022] 2. Uniformly disperse a layer of polystyrene microsphere dispersion liquid with a particle size of 1.5 μm on the surface of the glass sheet modified by the lysozyme nano-film, and then vacuum-dry to obtain a glass sheet adsorbing the polystyrene microsphere.

[0023] 3. Adjust 10mL of 40mmol / L tris(2-carboxyethyl)phosphine in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to pH 7.5 with NaOH, and then mix it wi...

Embodiment 3

[0025] In this embodiment, the polystyrene microspheres in Example 1 are replaced with silica microspheres with a particle size of 2 μm, and the other steps are the same as in Example 1. The obtained Janus microspheres are as follows: Figure 4 shown.

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Abstract

The invention discloses a method for preparing Janus particles based on a lysozyme nano-film. The method comprises the steps: modifying micro-nano-particles by adopting a phase-transition lysozyme nano-film so as to prepare the Janus particles with different particle sizes, shapes and material quality. The method disclosed by the invention has the advantages of simple and high-efficient modification processes, low cost, greenness, environmental protection; and the method has extremely high universality; the Janus micro-nano-particles of inorganic and organic polymer types can be prepared, andthe problems of poor universality and biocompatibility of a conventional modification method as well as harsh conditions, complicated steps and the like of the preparation method are overcome.

Description

technical field [0001] The invention relates to a green, environment-friendly, simple and efficient preparation method of Janus granules. Background technique [0002] Micro-nanoparticles have a wide range of applications, from catalysts to sensors, coatings, hybrid materials, optics, electronics, energy and biomedical probes. Among them, anisotropic particles have great potential as a class of colloidal materials with advanced functions. For example, Janus particles can be used as active pigments in novel display devices and photonic structures can be built through directional interactions. However, there are few methods that can successfully prepare Janus particles through a simple one-step method, such as interface modification based on Pickering emulsion, self-assembly of block copolymers, microfluidic synthesis, electroco-spraying, swelling emulsion polymerization . Therefore, it is urgent to develop a preparation method that is easy for large-scale industrial applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/06B01J13/02B82Y40/00B82Y30/00
CPCB01J13/02B01J13/06B82Y30/00B82Y40/00
Inventor 杨鹏刘瑞瑞
Owner SHAANXI NORMAL UNIV
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