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Phellinus igniarius fermentation medium

A fermentation medium and culture medium technology, applied in the field of Phellinus fermented medium, can solve the problems of low yield of Phellinus fermented mycelium, evaluation of Phellinus mycelium without mycelium biological activity, etc.

Inactive Publication Date: 2018-10-19
ZHEJIANG JOLLY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the existing production technology and literature reports, the yield of Phellinus mycelium fermentation is not high, and there is no biological activity on mycelium as an index to evaluate Phellinus mycelium

Method used

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  • Phellinus igniarius fermentation medium
  • Phellinus igniarius fermentation medium
  • Phellinus igniarius fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1 carbon source screening

[0010] 1.1 Medium

[0011] Shake flask seed medium: glucose 15-25g / L, peptone 5-15g / L, MgSO 4 ·7H 2 O0.5g / L, KH 2 PO 4 2g / L, VB10.1g / L, pH 6.0.

[0012] Carbon source screening basal medium: peptone 5-15g / L, MgSO 4 ·7H 2 O 0.5g / L, KH 2 PO 4 2g / L, VB10.1g / L, pH 6.0.

[0013] 1.2 Test method

[0014] First-level liquid strain culture: put 150mL shake flask seed culture medium in a 500mL conical flask, take the cultivated slant parent species into the conical flask, place it in a constant temperature shaker at 26-32°C, and cultivate at a speed of 140-180r / min for 7 -10d, that is, the first-class liquid bacteria.

[0015] Secondary liquid strain culture: put 150mL shake flask seed culture medium in a 500mL Erlenmeyer flask, absorb the first-grade liquid strain, and inoculate 15-30%. Place it on a constant temperature shaker at 26-32°C with a rotation speed of 140-180r / min, and cultivate for 2-3 days.

[0016] Shake flask ...

Embodiment 2

[0027] Example 2 Nitrogen Source Screening

[0028] 2.1 Medium

[0029] Shake flask seed medium: glucose 15-25g / L, peptone 5-15g / L, MgSO 4 ·7H 2 O0.5g / L, KH 2 PO 4 2g / L, VB 1 0.1g / L, pH 6.0.

[0030] Nitrogen source screening basal medium: glucose 15-25g / L, MgSO 4 ·7H 2 O 0.5g / L, KH 2 PO 4 2g / L, VB 1 0.1g / L, pH 6.0.

[0031] 2.2 Test method

[0032] The culture method of the first-level liquid strain and the second-level liquid strain is the same as 2.1. Shake flask fermentation culture: Take the cultured secondary liquid strains, and inoculate them in 6 different nitrogen source media according to the inoculum size of 10% (that is, add soybean meal, peptone, yeast powder, and , urea, beef extract and bran, the addition amount is 10-25g / L), placed in a constant temperature shaker at 26-32°C, with a rotation speed of 140-180r / min, cultured for 7-10d, and 3 parallel experiments to analyze different carbon sources Influence on Phellinus mycelium growth, get two bes...

Embodiment 3

[0050] The best combination of embodiment 3 carbon source and the screening of carbon-nitrogen ratio

[0051] 3.1 Orthogonal experiment

[0052] 3.1.1 Experimental scheme

[0053] According to the screening results of carbon and nitrogen sources, three carbon sources of corn flour, sucrose and glucose were selected as composite carbon sources in the experiment, and two nitrogen sources of soybean meal and yeast powder were selected as composite nitrogen sources. Orthogonal experiments were designed by using SPSS software. Orthogonal factors and The levels are shown in Table 3.1.1, 5 factors + 4 levels, to determine the best combination and ratio of carbon and nitrogen sources.

[0054] Table 3.1.1 Orthogonal test factors and content (g / L)

[0055]

[0056] The carbon-nitrogen ratio of the experimental medium is configured according to the above, and MgSO needs to be added to each group 4 ·7H 2 O 0.5g / L, KH 2 PO 4 1g / L, pH 6.0, placed in a constant temperature shaker a...

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Abstract

The invention provides a phellinus igniarius fermentation medium. The medium comprises the following components by weight: 10-15 parts of glucose, 15-20 parts of sucrose, 15-25 parts of corn powder, 10-20 parts of soybean meal, 0-10 parts of yeast powder, 5-10 parts of wheat bran powder, 0.25-1 part of MgSO4, 2 parts of Cacl2, 0.1-0.5 part of VB1, and a 4-8g / L mulberry twig aqueous extract. The medium can greatly improve the mycelial biomass and bioactivity of phellinus igniarius, the mycelial biomass is up to 3.0%, and other bioactivity indexes are as the following respectively: 0.26% for adenosine, 2.24% for polysaccharide, 0.37% for ergosterol, and 12.45% for mannitol. When the mulberry twig aqueous extract is added, the mycelial biomass can reach 3.5%. Therefore, the fermentation medium provided by the invention has the advantages of high mycelium biomass, high bioactivity, controllable quality, application to pilot scale production already and the like.

Description

technical field [0001] The invention belongs to the field of phellinus fermentation, and in particular relates to a phellinus fermentation medium. Background technique [0002] Phellinus is a class of very precious large fungi with important medicinal value, belonging to Basidiomyctina. It grows in a few places such as Japan, Philippines, Australia, China and North America. Phellinus often parasitizes on the dead wood of mulberry trees, and the fruiting bodies are perennial and woody. In China, Phellinus has been used for more than 2,000 years since the Han Dynasty. "Compendium of Materia Medica" records that Phellinus can "benefit the five internal organs, benefit the stomach, and expel toxins." It is regarded as a panacea for liver disease and cancer by the folks, and is known as "the best strain". [0003] Due to the particularity of the Phellinus growth, the number of natural Phellinus is very scarce, and the resources available for medicinal purposes in nature are li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 郑超一信文娟董调雅肖毓朱方剑姚瑶闻佳涛
Owner ZHEJIANG JOLLY PHARMA
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