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Culture solution for delaying aging of mesenchymal stem cells and preparation method thereof

A technology of mesenchymal stem cells and culture medium, applied in the field of biomedical materials, can solve the problems of loss of physiological functions, low success rate of culture, and decline of differentiation ability, etc., and achieve the effect of achieving a large number, prolonging the number of passages, and promoting the development of

Inactive Publication Date: 2018-10-19
广东芙金干细胞再生医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the application of mesenchymal stem cells in the fields of disease prevention and health care requires a large number of seed cells, but the acquisition of mesenchymal stem cells requires human tissue, and there are disadvantages such as low success rate of culture and easy aging of in vitro culture, resulting in cell Slowed proliferation, decreased differentiation capacity, and loss of physiological function

Method used

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Embodiment 1

[0031] An embodiment of the present invention provides a culture solution for delaying aging of mesenchymal stem cells, said culture solution for delaying aging of mesenchymal stem cells includes a basic culture solution and exogenous additives,

[0032] The exogenous additives are: dextran 5-15g / L, lipid concentrate 0.1-0.5mL / L, hydrocortisone 0.05-5μg / L, dexamethasone 0.1-10μg / L , sodium selenite is 1~5μg / L, β-mercaptoethanol is 10~50μM, vitamin C (VC) is 10~100mg / L, vitamin E (VE) is 10~50μg / L, transferrin is 1 ~20 mg / L, insulin 5~50 μg / L, growth hormone (GH) 10~50 μg / L, glutathione 10~50 μg / mL, non-essential amino acids 1%.

[0033] Further, the culture medium for delaying the aging of mesenchymal stem cells also includes cell growth factor addition components,

[0034] The added components of the cell growth factors are: basic fibroblast growth factor (bFGF) 1-25 μg / L, epidermal growth factor (EGF) 0.5-10 μg / L, transforming growth factor (TGF-β) 1-10 μg / L, platelet-der...

Embodiment 2

[0039]The embodiment of the present invention also provides a preparation method of a culture medium for delaying the aging of mesenchymal stem cells, the preparation method of the culture medium for delaying the aging of mesenchymal stem cells comprises:

[0040] Use ultrapure water to prepare basal culture medium;

[0041] Add dextran to the basic culture medium and stir to dissolve, then add lipid concentrates, hydrocortisone, dexamethasone, sodium selenite, β-mercaptoethanol, vitamin C (VC), vitamin E ( VE), transferrin, insulin, growth hormone (GH), glutathione, non-essential amino acids, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), transforming growth factor (TGF-β) , platelet-derived growth factor (PDGF), stem cell growth factor (SCF), adjusted to pH 7.2, sterilized with filter membrane after constant volume, and stored at 4°C.

[0042] Further, using the mesenchymal stem cell culture medium of the present invention to cultivate primary cells i...

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Abstract

The embodiment of the invention provides a culture solution for delaying aging of mesenchymal stem cells and a preparation method thereof, and relates to the technical field of biomedical materials. The culture solution reduces the aging rate of the mesenchymal stem cells as much as possible, maintains cell phenotypes and functions of the stem cells and improves the utilization efficiency of the stem cells while in-vitro proliferation of the mesenchymal stem cells is increased, so that requirements of scientific research and clinical application are met. According to the culture solution for delaying aging of the mesenchymal stem cells and the preparation method thereof, the culture solution comprises a basal culture solution and exogenous additives, wherein the exogenous additives include5-15g / L of glucan, 0.1-0.5mL / L of a lipid-based concentrating agent, 0.05-5[mu]g / L of hydrocortisone, 0.1-10[mu]g / L of dexamethasone, 1-5[mu]g / L of sodium selenite, 10-50[mu]M of beta-mercaptoethanol, 10-100mg / L of vitamin C (VC), 10-50[mu]g / L of vitamin E (VE), 1-20mg / L of transferrin, 5-50[mu]g / L of insulin, 10-50[mu]g / L of growth hormone (GH), 10-50[mu]g / mL of glutathione and 1% of non-essential amino acids.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a culture solution and a preparation method for delaying aging of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are a type of tissue stem cells with multi-directional differentiation potential, which can proliferate and differentiate in the directions of osteogenesis, cartilage, fat, nerve and cardiomyocytes. Based on the extensive plasticity of this type of cells, extensive application research has been carried out in numerous animal disease models and clinical trials. [0003] At present, researchers have isolated and obtained mesenchymal stem cells from various tissues of animals and humans, such as: bone marrow (BMSCs), fat (ADSCs), umbilical cord (UC-MSCs), umbilical cord blood (UB-MSCs), dental pulp , amnion, placenta and synovium. Mesenchymal stem cells from different tissue sources have certain d...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N2500/12C12N2500/25C12N2500/34C12N2500/36C12N2500/38C12N2500/44C12N2501/11C12N2501/115C12N2501/125C12N2501/135C12N2501/15C12N2501/305C12N2501/39C12N2501/999
Inventor 田智泉程蕊苹徐丹丹邓志宏赵宇雷娟
Owner 广东芙金干细胞再生医学有限公司
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