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Method for detecting nicotine based on fluorescence resonance energy transfer

A technology of nicotine and fluorescence intensity, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of poor selectivity and low sensitivity, and achieve the effect of simple instrument, simple operation method and simple operation.

Inactive Publication Date: 2018-10-19
CHINA TOBACCO YUNNAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing nicotine analysis and detection methods have gravimetric analysis, titration analysis, optical rotation, infrared spectroscopy quantitative analysis, atomic absorption spectrophotometry, chromatographic analysis, potential analysis, polarographic analysis, etc., and these methods each Pros and cons, but generally less sensitive and less selective

Method used

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  • Method for detecting nicotine based on fluorescence resonance energy transfer
  • Method for detecting nicotine based on fluorescence resonance energy transfer
  • Method for detecting nicotine based on fluorescence resonance energy transfer

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: the determination of nicotine in cigarette sample

[0037] 1. Preparation of water-soluble carbon quantum dot solution: Weigh 10.0g of polyethyleneimine liquid into 90mL of ultrapure water, sonicate for 5 minutes to make it fully mixed, and transfer to a polytetrafluoroethylene reaction kettle, heat at 200°C After cooling down to room temperature naturally for 5 hours, filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3500D for 24 hours to obtain a water-soluble carbon quantum dot solution.

[0038] 2. Preparation of water-soluble metal quantum dot solution: 50mL 0.01mol / L CdCl 2 Add the solution and 250 μL of thioglycolic acid into a 250 mL three-neck flask, mix well under magnetic stirring, slowly add 1.0 mol / L NaOH to adjust the pH to 11, and 2 After stirring for 30min under protection to remove the oxygen in the mixture, add 5mL of 0.1mol / L Na 2 S·9H ...

Embodiment 2

[0042] Example 2 Determination of Nicotine in Electronic Cigarette Liquid

[0043] 1. Preparation of water-soluble carbon quantum dots: same as in Example 1.

[0044] 2. Preparation of water-soluble metal quantum dots: same as in Example 1.

[0045] 3. Preparation of nicotine working curve: Transfer 0.5mL of 1, 20, 40, 60, 80, 100mg / L nicotine standard solution to a 10mL stoppered colorimetric tube, and add 30μL of the water-soluble metal obtained in step 2 in sequence Quantum dot solution, 70 μL of the water-soluble carbon quantum dot solution obtained in step 1, diluted to the mark with a pH 6.0 phosphate buffer solution, shaken well, and left to stand for 8 minutes, with 300nm as the excitation wavelength, the excitation and emission slits are both 5nm, at Fluorescence intensity measured at 600nm, the regression equation (F-F 0 ) / F 0 =0.0298C(μg / mL)+0.0301, correlation coefficient r=0.9990, where F and F 0 Represent the fluorescence intensity of metal quantum dots in th...

Embodiment 3

[0048] The mensuration of nicotine in the tobacco leaf sample of embodiment 3

[0049] 1. Preparation of water-soluble carbon quantum dots: same as in Example 1.

[0050] 2. Preparation of water-soluble metal quantum dots: same as in Example 1.

[0051] 3. Preparation of nicotine working curve: Transfer 0.5mL of 1, 20, 40, 60, 80, 100mg / L nicotine standard solution to a 10mL stoppered colorimetric tube, and add 40μL of the water-soluble metal obtained in step 2 in sequence Quantum dot solution, 80 μL of the water-soluble carbon quantum dot solution obtained in step 1, diluted to the mark with a pH 6.0 phosphate buffer solution, shaken well, and left to stand for 10 min, with 300 nm as the excitation wavelength, the excitation and emission slits are both 5 nm, at Fluorescence intensity F measured at 600nm place, obtain regression equation (F-F 0 ) / F 0 =0.1203C(μg / mL)+0.1230, correlation coefficient r=0.9992, where F and F 0 Represent the fluorescence intensity of metal quan...

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Abstract

The invention discloses a method for detecting nicotine based on fluorescence resonance energy transfer. The method comprises the following steps: (1) fitting of a regression equation: adding a water-soluble metal quantum dot solution and a water-soluble carbon quantum dot solution having certain concentrations and volumes into several nicotine standard solutions having different concentrations, diluting to a certain volume with a weak acid solution, and then respectively measuring fluorescence intensities F and F0 to obtain a regression equation of a relationship between (F-F0) / F0 and a nicotine concentration, wherein F and F0 are respectively the fluorescence intensities of metal quantum dots in the presence and absence of nicotine; (2) sample measurement: measuring F and F0 of a nicotine solution having unknown concentration, and substituting the obtained F and F0 into the regression equation obtained in step (1) to calculate the content of nicotine in samples having unknown concentration. The method provided by the invention utilizes the fluorescence resonance energy transfer between carbon quantum dots and metal quantum dots to propose a novel method for detecting nicotine; the method has advantages of being good in stability, high in sensitivity, good in selectivity, simple in operation method, and green.

Description

technical field [0001] The invention relates to a method for detecting nicotine based on fluorescence resonance energy transfer. It belongs to the technical field of detection. Background technique [0002] Nicotine, also known as nicotine, belongs to the pyridine family of alkaloids and is a unique component in tobacco. In recent years, with the rapid development of medicine, tobacco industry, agriculture, chemical industry and many other fields, the demand for natural nicotine in the market is increasing day by day. Quick, simple and accurate determination of nicotine content in nicotine samples and nicotine products is necessary for enterprise production. Existing nicotine analysis and detection methods have gravimetric analysis, titration analysis, optical rotation, infrared spectroscopy quantitative analysis, atomic absorption spectrophotometry, chromatographic analysis, potential analysis, polarographic analysis, etc., and these methods each There are pros and cons,...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 王程娅尚善斋汤建国杨亚玲陈永宽冒德寿郑绪东李志强吴俊王蒙成经辉马晓龙
Owner CHINA TOBACCO YUNNAN IND
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