Multiplexed single-cell proteomics monitoring method based on sprm technology

Abstract

A multiplexed single-cell proteomics monitoring method based on SPRM technology. Around the capture site in the first fixed-point capture chip, the specific recognition antibodies of different proteins are modified in the form of radial lattice; injected into the micropipe The cell solution to be detected makes it produce different SPR response signals at the single cell capture site; screen out 3-6 secreted protein markers of the cells to be tested with strong SPR response signals; the single cell in the second fixed-point capture chip Around the cell capture site, different antibodies are modified in a fan-shaped structure, and the cell solution to be detected is injected into the micropipeline; barcode-shaped specific recognition antibodies for different proteins are respectively modified on both sides of the fan-shaped structure in the second fixed-point capture chip; The pipeline is injected with cell lysate, and the strength of the SPR response signal at different barcodes is compared to achieve high-sensitivity monitoring. The invention can realize the in-situ and dynamic monitoring of various secreted proteins, and improve the adsorption and fixation amount of protein molecules and the detection sensitivity.

Description

technical field [0001] The invention relates to a single-cell proteomics monitoring method. In particular, it relates to a multiplexed single-cell proteomics monitoring method based on SPRM technology. Background technique [0002] The proteome can not only provide the material basis for the laws of life activities, but also provide a theoretical basis and solutions for the elucidation and overcoming of the mechanisms of many diseases. Through the comparative analysis of proteomes between normal individuals and pathological individuals, some "disease-specific protein molecules" can be found, which can become molecular targets for new drug design, and can also provide molecular markers for early diagnosis of diseases. Cellular heterogeneity is a property of great interest in basic medicine and disease research. Single-cell measurement helps to gain a deep understanding of this characteristic, and establishes the relationship between cellular function / phenotype and extracell...

AI Technical Summary

Problems solved by technology

However, through the existing spotting instrument, electropolymerization system, etc., only antibody sites with a diameter of hundreds of microns can be modified on the surface of the gold film of the SPRi sensor, so the current SPRi technology can only be used for the secretion of protein solutions or cell groups Detection of protein / intracellular protein, unable to achieve single-cell multiplexed multi-protein monitoring

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