A method for determining the content of gluconic acid in food
A technology of gluconic acid and content, applied in the field of gluconic acid detection, can solve the problems of low reaction sensitivity, high measurement cost, long measurement time, etc., and achieve the effects of high sensitivity, short reaction time, and short detection period
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0014] Embodiment 1: a kind of method for measuring gluconic acid content in food, the steps are as follows:
[0015] (1) To make a standard curve: Measure 3mL of edible indigo solution and add water to 10mL as a control solution. The concentration of gluconic acid stock solution is 3.6mM, the concentration of ferrous sulfate stock solution is 5.39mM; measure 0, 10, 100, 500, 1200, 2000, 2500 μL of gluconic acid stock solution, add 0.3mL of ferrous sulfate stock solution solution, adjust the pH of the solution to 11.52 with phosphate buffer solution, add 2 mL of edible indigo with a concentration of 0.43 mM, add water to 10 mL, and react for 1 minute. Measure the absorbance of the solution at a wavelength of 610 nm. Taking the concentration c of gluconic acid as the abscissa and the absorbance A of edible indigo at a specific wavelength as the ordinate, a standard curve and a linear regression equation were established.
[0016] (2) Sample determination. Take 2.5612g of com...
experiment example 1
[0017] Experimental example 1: Experiment on the influence of pH value in the reaction system: add 0.4mL ferrous sulfate solution to 1.2mL gluconic acid, adjust the pH value of the solution to 8-13 with phosphate buffer solution, then add 3mL edible indigo solution and add water The absorbance value of the solution at a wavelength of 610nm after reacting to 10mL for 1 minute, the results are shown in Table 1, and the line graph is shown in figure 1 . Among them: edible indigo 0.086mM, gluconic acid 0.432mM, ferrous sulfate 0.216mM, phosphate buffer saline 0.1M.
[0018] Table 1
[0019]
[0020] from table 1 and polyline figure 1 It can be seen that when the pH value of the system is 11.52, the absorbance value of the solution is the smallest, so the pH value of the system is set as 11.52.
experiment example 2
[0021] Experimental example 2: system pH regulator selection experiment: replace the phosphate buffer solution in the experimental example 1 experiment with sodium hydroxide to adjust the pH value of the solution to 11.52, and other conditions remain unchanged. After testing, the absorbance of the solution is measured at a wavelength of 610nm A=1.2419. From the result of example 2, it can be seen that when the pH regulator is changed from phosphate buffer to sodium hydroxide and the pH value is constant, the absorbance of the system is obviously larger, so the pH regulator is selected as phosphate buffer.
PUM
| Property | Measurement | Unit |
|---|---|---|
| absorbance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More