Application of 25-hydroxyvitamin D3 in foods
A technology of hydroxyvitamin and food, which is applied in the field of nutrition fortification and/or fortified food, and can solve the problems of inability to increase and decrease of 25-hydroxyvitamin D concentration, etc.
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Embodiment 1
[0064] Example 1: Contains 25-hydroxyvitamin D 3 Preparation of liquid fortified foods
[0065] Under the protection of argon, 25-hydroxyvitamin D monohydrate with a content of 99.8% 3 100 g by weight is dissolved in 600 g of absolute ethanol, 300 g of BHA is added to prepare an ethanol solution with a weight ratio of 10%, and a certain amount of folic acid is added. Add the edible oil of 99kg wherein again, prepare the monohydrate 25-hydroxyvitamin D that contains 40,000 I.U / g 3 oily solvent, and the content is detected by high performance liquid phase, and the relative deviation is controlled within 3%. Under nitrogen atmosphere, subpackage into 1kg / bottle and store at room temperature.
[0066]
[0067]
[0068] Content determination method:
[0069] Liquid phase conditions:
[0070] Device: high phase liquid phase, equipped with UV detector.
[0071] Chromatographic column: waters C 18 , 4.6mm×150mm, 5μm.
[0072] Mobile phase: methanol:water=800:200 (V:V). ...
Embodiment 2
[0085] Example 2: Containing 25-hydroxyvitamin D 3 Preparation of Fortified Margarine
[0086] 25-hydroxyvitamin D prepared by the 16th test in embodiment 1 3 Add 2.5g (40,000 I.U / kg) of the liquid enhancer product to the fat-soluble additives (80kg) for preparing margarine, then add all water-soluble additives (20kg), and vigorously stir and emulsify with a homogenizer. Control the temperature to 70-80°C, make the watery substance become small water droplets and evenly disperse in the oily substance, pump the emulsion into the heat exchanger, and then let the emulsion pass through the cooled crystallizer and squeeze it continuously compressed into blocks and packaged to contain 1000I.U / kg25-hydroxyvitamin D 3 margarine.
Embodiment 3
[0087] Example 3: 25-Hydroxyvitamin D in solid form 3 Preparation of food fortifier
[0088] 25-Hydroxyvitamin D in solid form 3 The preparation of the food fortifier comprises the following steps:
[0089] (1) Under argon protection, 25-hydroxyvitamin D monohydrate with a content of 99.8% 3 100 g by weight was dissolved in 200 g of absolute ethanol, 300 g of BHA was added to prepare an ethanol solution with a weight ratio of 16.6%, and a certain amount of 1.5 kg of folic acid was added. Then add 7.9kg of edible oil therein to prepare an oily solvent containing 400,000 I.U / g of monohydrate 25-hydroxyvitamin D3, and detect the content with high performance liquid phase, the relative deviation is controlled within 3%, and A liquid is obtained;
[0090] (2) Dissolve 11kg of maltodextrin, 77kg of modified starch, 1kg of sorbic acid, and 1kg of sodium benzoate in 110kg of water, mix well, and keep the water temperature at about 45°C to obtain liquid B;
[0091] (3) Mix liquid A...
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