Preservation method of phellinus linteus

A technology of Phellinus fungus and mycelia, which is applied in the field of microbial preservation, can solve the problems of low equipment and operation technology requirements, low cost, etc., so that the growth of mycelium and cultivation characteristics of the strain are not affected, the cost is low, and the application prospect is good Effect

Active Publication Date: 2018-11-06
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, in the field of Phellinus preservation technology, there is still a lack of low cost, low requirements for equipment and operation technology, and a preservation method that can retain the excellent genetic properties of Phellinus strains for a long time

Method used

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  • Preservation method of phellinus linteus
  • Preservation method of phellinus linteus
  • Preservation method of phellinus linteus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of medium:

[0038] (1) Preparation of sawdust: The tung trees and maple trees cut from the mountain are sawn into sawdust respectively, the particle size of the tung tree chips is long (0.5-1.5mm) × wide (0.1-0.5mm); the maple wood chips are Diameter length (0.5-1.5mm) × width (0.1-0.5mm), dried in the sun (moisture content below 10%).

[0039] (2) Weigh the dried sawdust, mix it uniformly in proportion, then add wheat bran and corn cob powder and mix uniformly, then add the mixed trace element auxiliary materials and mix uniformly, then add water and mix well. The specific ratio is as follows:

[0040] M1: 50% tung tree chips + 15% maple wood chips + 25% wheat bran + 10% corn cob flour; M2: 16% magnesium sulfate + 34% potassium dihydrogen phosphate + 50% gypsum powder, M1: M2: water =1:0.03:1.4.

[0041] (3) Put the well-mixed culture medium into a 25mm×200mm glass test tube, the filling volume accounts for about three-fifths of the length of the test t...

Embodiment 2

[0045] Preparation and use of Phellinus preserved strains:

[0046] (1) Inoculate the mother species of Phellinus fungus on the culture medium of a glass plate, seal the edge with a parafilm, and culture it in an incubator at a constant temperature of 26°C until the mycelia cover two-thirds of the plate.

[0047] (2) insert the long Phellinus mycelium into the sterilized test tube of Example 1, put in the incubator for constant temperature cultivation at 26°C, and when the mycelium grows to two-thirds of the test tube (the mycelium grows into About 1 cm in the sawdust, remove the mycelium and fungus skin above the sawdust, and then let it grow at a constant temperature).

[0048] (3) More than half of the test tubes with Phellinus mycelium growing well were randomly selected to inoculate the slope of the fresh test tube for verification test. After the inoculation, all the test tubes were wrapped with kraft paper, put into ziplock bags, and stored in the refrigerator at 4°C. ...

Embodiment 3

[0052] Effects of Preserved Phellinus Phellinus Strains on Mycelia Growth and Cultivation Characteristics

[0053] According to embodiment 1, embodiment 2 technical scheme preparation Phellinus Phellinus preserved bacterial classification carries out mycelia growth characteristics and artificial cultivation test. Test bacterial classification: Phellinus test tube species (slope: control group), Phellinus preserved species of the present invention (test group: storage period is respectively 6 months, 1 year, 2 years, 3 years).

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Abstract

The invention relates to a preservation method of phellinus linteus. A main material of a culture medium is prepared from the following components by mass: 50% of tung tree wood dust, 15% of maple tree wood dust, 25% of wheat bran, and 10% of corn cob powder; an auxiliary material is prepared from the following components by mass: 16% of magnesium sulfate, 34% of potassium dihydrogen phosphate, and 50% of gypsum powder; a formula of the culture medium of a wood dust tube comprises the main material, the auxiliary material and water according to a ratio of 1:0.003:1.4. The preparation method comprises the following steps of firstly, uniformly mixing the two types of wood dust in the main material, sequentially adding the wheat bran and the water, and uniformly mixing; adding the mixed auxiliary material, uniformly mixing, adding water and uniformly stirring; separately loading the culture medium into test tubes, packaging, sterilizing, and inoculating, so as to obtain the phellinus linteus preservation strain when the phellinus linteus can well grow and be free from infection by infectious microbes after re-transfer verification. The preservation method has the advantages that the effect is excellent, and the preserved strain can be effectively preserved for 2 to 5 years; after activating, the growth rate of hypha, growth condition, biology conversion rate and the like are basically free from influence.

Description

technical field [0001] The invention relates to the technical field of microorganism preservation, in particular to a method for preserving Phellinus spp. Background technique [0002] Phellinus is a rare large medicinal fungus, belonging to Basidiomycota, Polyporaceae, and Inonotus. Its main functions include treating dysentery, night sweats, metrorrhagia, bloody stranguria, astringent pain in the navel and abdomen, anal prolapse and diarrhea, leukorrhea, amenorrhea, antidiarrheal, prolonging life, etc., so it has the reputation of "forest gold". Phellinus Phellinus is currently internationally recognized as a medicinal fungus with the best biological cancer treatment effect, and has attracted widespread attention from international scientific research departments, pharmaceutical circles and health care products industries. [0003] Phellinus fine strains are the basis and key of its production, so it is necessary to effectively preserve the strains to ensure the excellent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/645
CPCC12N1/04
Inventor 吴胜莲邵晨霞唐少军许隽杨祎靳磊葛小鹏贺月林
Owner HUNAN INST OF MICROBIOLOGY
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