Staphylococcus aureus detection membrane, preparation method and bacterial detection method thereof
A staphylococcus, golden yellow technology for nanotechnology for materials and surface science, measurement devices, nanotechnology for sensing, etc.
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Embodiment 1
[0044] 1) Preparation of nanofiber film: uniformly spray polyethylene-polyvinyl alcohol nanofiber suspension on the surface of ordinary non-woven fabrics, dry after film formation, and separate to prepare polyvinyl alcohol- Polyethylene nanofibrous membrane;
[0045] 2) Prepare a surface-activated nanofiber membrane: immerse the nanofiber membrane prepared in step 1) in a sodium hydroxide solution with a concentration of 1 mol / L for about 30 minutes to obtain a surface-activated nanofiber membrane;
[0046] 3) Preparation of bacteria detection membrane: immerse the surface-activated nanofibrous membrane prepared in step 2) in an aqueous solution of glycosyl polymer functionalized with fluorescein-based fluorescent molecules at a concentration of 3 g / L for 20 minutes, and then take out After being washed with clean water, it was immersed in an aqueous solution of phenylboronic acid polymer with a concentration of 3g / L for 20 minutes, then taken out and washed with clean water, ...
Embodiment 2
[0048] 1) Preparation of nanofiber film: uniformly spray polyethylene-polyvinyl alcohol nanofiber suspension on the surface of ordinary non-woven fabrics, dry after film formation, and separate to prepare polyvinyl alcohol- Polyethylene nanofibrous membrane;
[0049] 2) Prepare a surface-activated nanofiber membrane: immerse the nanofiber membrane prepared in step 1) in a sodium hydroxide solution with a concentration of 0.5 mol / L for about 30 minutes to obtain a surface-activated nanofiber membrane;
[0050] 3) Preparation of bacteria detection membrane: immerse the surface-activated nanofiber membrane prepared in step 2) in an aqueous solution of glycosyl polymer functionalized with fluorescein-like fluorescent molecules at a concentration of 1 g / L for 30 minutes, and then take out After being washed with clean water, it was immersed in an aqueous solution of phenylboronic acid polymer with a concentration of 2g / L for 30 minutes, then taken out and washed with clean water, a...
Embodiment 3
[0052] 1) Preparation of nanofiber film: uniformly spray polyethylene-polyvinyl alcohol nanofiber suspension on the surface of ordinary non-woven fabrics, dry after film formation, and separate to prepare polyvinyl alcohol- Polyethylene nanofibrous membrane;
[0053] 2) Prepare a surface-activated nanofiber membrane: immerse the nanofiber membrane prepared in step 1) in a sodium hydroxide solution with a concentration of 2.5 mol / L for about 30 minutes to obtain a surface-activated nanofiber membrane;
[0054] 3) Preparation of bacteria detection membrane: immerse the surface-activated nanofiber membrane prepared in step 2) in an aqueous solution of glycosyl polymer functionalized with fluorescein-based fluorescent molecules at a concentration of 4 g / L for 20 minutes, and then take out After being washed with clean water, it was immersed in an aqueous solution of phenylboronic acid polymer with a concentration of 4g / L for 20 minutes, then taken out and washed with clean water, ...
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Abstract
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