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Method for testing content of sodium nitrite in food

A technology of sodium nitrite and content, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of high measurement cost and long measurement time. , low reaction sensitivity and other problems, to achieve the effect of reducing the cost of measurement

Active Publication Date: 2018-11-06
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a method for measuring sodium nitrite content in food in order to solve the problems of complex operation, long measuring time, low reaction sensitivity and high measuring cost in the existing method for measuring sodium nitrite

Method used

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  • Method for testing content of sodium nitrite in food
  • Method for testing content of sodium nitrite in food
  • Method for testing content of sodium nitrite in food

Examples

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Embodiment 1

[0013] Embodiment 1: A method for measuring sodium nitrite content in food, adding sodium nitrite solution to the mixed solution of indigo and hydrogen peroxide and shaking reaction, wherein in the reaction system: the concentration of indigo is 00007M ~ 0.0035M, hydrogen peroxide The concentration is 0.0008M~0.0063M, the concentration of sodium nitrite is 0.07μM~0.07mM, the pH value of the system is 1~4, and the absorbance value at the wavelength of 695nm is measured after the reaction. Establish a standard curve and a linear regression equation on the ordinate, then add the solution of the analyte to the same mixed system of indigo and hydrogen peroxide to react, measure the absorbance value at a wavelength of 695nm, and obtain the concentration of the analyte through the linear regression equation and the standard curve. Sodium nitrite content.

[0014] The specific steps for making a standard curve are: A. Measure 0.3 mL of DL solution and add water to 10 mL, as a control ...

experiment example 1

[0016] Experimental Example 1: The influence of different pH values ​​on the absorbance of the solution: when the concentration of indigo is 0.108mM, the concentration of hydrogen peroxide is 27μM, and the concentration of sodium nitrite is 3.5μM, the pH of the solution is adjusted to 1.01, 2.05, 3.10, 4.15 respectively , 5.05, the absorbance A of the solution at a specific wavelength is as follows:

[0017] Table 1

[0018]

[0019] The absorbance line graph of the system under different pH conditions is shown in figure 1 . From the data in Table 1 and figure 1 It can be seen that when the pH of the system is greater than 5, the absorbance value of the solution increases, so the pH of the system is set at 1-4.

experiment example 2

[0020] Experimental Example 2: The influence of different ratios of hydrogen peroxide and sodium nitrite on the absorbance of the solution: when the concentration of indigo is 108 μM and the pH of the solution is adjusted to 1.01, when the ratio of hydrogen peroxide and sodium nitrite is different, the concentration of the solution at a specific wavelength The absorbance is shown in Table 2. For a line graph with different ratios of hydrogen peroxide and sodium nitrite, see figure 2 , the ultraviolet spectrum of indigo with the increase of sodium nitrite concentration is shown in Figure 5 . Depend on Figure 5 It can be seen that with the increase of the concentration of sodium nitrite, the absorbance value of the solution decreases. The results show that when the ratio of hydrogen peroxide and sodium nitrite is 10:1 to 13:1, the absorbance value of the system is smaller than that of other ratios, so the ratio of hydrogen peroxide and sodium nitrite in the system is set a...

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Abstract

The invention belongs to the technical field of rapid sodium nitrite testing methods, aims to solve the problems that a conventional sodium nitrite testing method is complex and tedious in operation,long in testing time, low in reaction sensitivity, high in testing cost, and the like, and provides a method for testing the content of sodium nitrite in a food. The method comprises the following steps: putting a mixed solution of indigo blue and hydrogen peroxide into a sodium nitrite solution, and carrying out an oscillation reaction; after the reaction, testing an absorbance value at a wavelength of 695nm; by taking a concentration of the sodium nitrite as a transverse coordinate and the absorbance value as a longitudinal coordinate, establishing a standard curve and an equation of linearregression; putting a solution of a substance to be tested into a same mixed system of indigo blue and hydrogen peroxide, carrying out a reaction, testing an absorbance value at the wavelength of 695nm, and testing the content of the sodium nitrite in the substance to be tested according to the equation of linear regression and the standard curve. The method can be widely applied to rapid, simpleand accurate detection on the contents of sodium nitrite in foods, is simple to operate and is accurate and sensitive.

Description

technical field [0001] The invention belongs to the technical field of fast determination methods for sodium nitrite, and in particular relates to a method for determining the content of sodium nitrite in food, which uses hydrogen peroxide and sodium nitrite to react to produce a strong oxidant, peroxynitrite, to oxidize indigo and fade color to determine sodium nitrite visible spectrophotometry. Background technique [0002] Sodium nitrite is a kind of industrial salt, which is very similar to table salt sodium chloride, but it is poisonous and cannot be eaten. Sodium nitrite is highly toxic. People may experience symptoms of poisoning if they consume 0.2 to 0.5 grams. If they eat 3 grams at one time, they may die. Sodium nitrite is widely used in cured meat. It is produced according to GB1907 national standard as a food additive and added in accordance with GB2760. The maximum amount used in meat is 0.15g / kg, and the residual amount of sodium nitrite in meat should not ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 兰艺凤卫艳丽宋秀丽董川
Owner SHANXI UNIV
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