A method for simultaneously and efficiently extracting chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol multi-residue from poultry eggs
A technology of florfenicolamide and thiamphenicol, which is applied in the field of veterinary drug residue detection and can solve the problems of low recovery rate of florfenicolamide and the like
Active Publication Date: 2019-12-10
江苏农邦禽业有限公司
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Problems solved by technology
[0003] In order to solve the problem of low recovery rate of chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine in the extraction of poultry eggs, the present invention provides a method of extracting poultry eggs simultaneously and efficiently The method of extracting chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine with many residues can avoid errors and poor reproducibility caused by manual extraction, and is time-consuming and suitable for batch processing sample
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[0036] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.
[0037]The present invention will be described in further detail below in conjunction with specific examples and with reference to data. These examples are only for illustration of the present invention and do not limit the scope of the present invention in any way.
[0038] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art. The sources and trade names of the reagents used, as well as those whose components must be listed, are indicated when they appear for the first time, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.
[0039] In order to solve the problem of low recovery rate of chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine in the extraction ...
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Abstract
The invention relates to the field of detection of veterinary drug residues, in particular to a method for efficiently extracting chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine residues from poultry eggs simultaneously, comprising the following steps: Egg sample extraction and purification: 97:2:1, V / V methanol:ammonia:water solution is used as the extractant, accelerated solvent extraction ASE technology extraction, centrifugal concentrator evaporation concentration, n-hexane manual degreasing; liquid chromatography-tandem Mass spectrometry detection. The present invention has selected methanol: ammonia water: ultrapure water (97:2:1, V / V) as extraction agent, and accelerated solvent extraction method simultaneously extracts chloramphenicol, thiamphenicol, florfenicol and its The metabolite florfenicol amine is more residual. Chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine are added to poultry eggs at the concentration of LOQ, 0.5MRL, 1.0MRL, and 2.0MRL, respectively, and the extraction recovery rates are greater than or equal to 90.31%. , 93.40%, 92.32% and 92.35%, meeting the requirements of veterinary drug residue detection methods.
Description
technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to a method for simultaneously and efficiently extracting chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine residues from poultry eggs with an accelerated solvent extraction apparatus . Background technique [0002] When detecting veterinary drugs in food of animal origin, only by extracting the drugs from the samples can an efficient detection method be established. Pure chloramphenicol is neutral white or slightly yellow-green needle-shaped, long flaky crystals or crystalline powder, with extremely bitter taste and stable properties, with a melting point of 149-151°C. Slightly soluble in water, slightly soluble in ethylene glycol, insoluble in benzene and petroleum ether, but easily soluble in methanol, ethanol, propylene glycol, acetone, ethyl acetate and other organic solvents, and the solubility in ethanol can reach 400mg / mL. ...
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 谢恺舟王波赵霞谢星庞茂达王雅娟郭亚文卜晓娜刘楚君张跟喜张涛戴国俊王冉卜仕金刘学忠王金玉
Owner 江苏农邦禽业有限公司