A kind of low-temperature degradation straw compound bacteria agent containing lactic acid bacteria and its application
A compound bacterial agent and lactic acid bacteria technology, applied in the field of microorganisms, can solve the problems of farmers' autumn ploughing, short sowing time, blockage of rivers and road traffic, and blockage of market information, so as to improve food digestibility and biological titer, and maintain micro-ecological balance. , the effect of increasing appetite
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] The mutagenic screening of embodiment 1 verrucous Myromyces
[0021] (1) Morphological identification and screening
[0022] The soil in the forest area of Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.
[0023] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.
[0024] At the same time, in order to directional screen lignin-deg...
Embodiment 2
[0030] The low-temperature acclimatization of embodiment 2 bacterial strains
[0031] (1) Enrichment culture strains
[0032] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L, enrichment culture for 4 generations.
[0033] (2) Preliminary screening and domestication of low-temperature cellulose-degrading composite strains
[0034] Using the filter paper disintegration method, the above-mentioned enriched culture was inoculated into the filter paper strip medium to...
Embodiment 3
[0044] The preparation of embodiment 3 composite bacterial agents
[0045] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Bacteria and lactic acid bacteria were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial solution reached 2.0×10 8 individual / mL.
[0046] Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 15% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% Bacillus plasmogenus, 10% manganese peroxidase-producing Laysia actinomycetes, 25% lactic acid bacteria, fully stirred and mixed to obtain a mixed bacterial liquid.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com