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Chitin deacetylase and chitin nanofiber dispersion liquid as well as preparation method and application of chitin nanofiber dispersion liquid

A deacetylase and nanofiber technology, applied in the field of chitin nanofiber preparation, can solve the problems of unstable product quality, expensive enzyme, cumbersome operation, etc., achieve long reaction time, large environmental benefits, and reduce degradation Effect

Inactive Publication Date: 2018-11-13
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing chitin deacetylases still have many defects and deficiencies in the field of preparing nano-chitin, such as unstable product quality, expensive and difficult to obtain enzymes, high cost, cumbersome operation, etc.

Method used

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  • Chitin deacetylase and chitin nanofiber dispersion liquid as well as preparation method and application of chitin nanofiber dispersion liquid
  • Chitin deacetylase and chitin nanofiber dispersion liquid as well as preparation method and application of chitin nanofiber dispersion liquid

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preparation example Construction

[0057] In a third aspect, in at least one embodiment, a method for preparing a chitin nanofiber dispersion is provided, comprising the following steps:

[0058] Using the above-mentioned chitin deacetylase to partially deacetylate chitin to obtain partially deacetylated chitin;

[0059] Partially chitinized chitin is processed by mechanical action to obtain a chitin nanofiber dispersion.

[0060] The present invention applies chitin deacetylase to the partial deacetylation modification of chitin, and proposes a method for preparing chitin nanofiber dispersion liquid by biological enzymatic method, which avoids the pollution of the environment by the traditional concentrated alkali pyrolysis method, Moreover, the degradation effect of chitin is reduced, the reaction system is mild and environmentally friendly, and chitin nanofibers with high aspect ratio can be obtained; it provides new ideas and methods for the preparation of chitin nanofibers.

[0061] The chitin deacetylase...

Embodiment approach

[0067] As a preferred embodiment of the present invention, the preparation method of chitin nanofiber dispersion comprises the following steps:

[0068] (1) Prepare liquid culture medium of 5~15g / L glucose, 5~15g / L peptone, 3~8g / L yeast extract, insert Penicillium oxalicum 3.5705 strain into activated liquid medium, at 24~36°C, Oxygen consumption, the shaker speed is 160-240rpm, and the activation time is 12-24 hours to activate the Penicillium oxalicum 3.5705 strain;

[0069] (2) Prepare 5-20g / L glucose, 1-10g / L peptone, 1-10g / L yeast extract, 1-5g / LKH 2 PO 4, 0.5~1.5g / L K 2 HPO 4 , 0.1~2.0g / L (NH 4 ) 2 SO 4 , 0.1~1g / L MgSO 4 , 0.1~1g / L NaCl and 0.1~1g / L CaCl 2 0.05-0.2% Tween 80 was added; the activated Penicillium oxalicum 3.5705 strain was inserted into the fermentation liquid medium at 5-20%, and the initial pH of the fermentation broth was 5.0-7.0 at 24-36°C. Oxygen consumption, the rotating speed of the shaker is 160-240rpm, and the fermentation enzyme producti...

Embodiment 1

[0081] A preparation method of chitin nanofiber dispersion liquid, comprising the following steps:

[0082] (1) The source and purification of chitin: Crab shell (Portunus trituberculatus), produced in Nantong, China, the purification method of chitin in crab shell: 1) Cut the crab shell into 1cm 2 Size, soak in 1mol / L NaOH for more than 12 hours, then wash with distilled water to neutral to remove protein in crab shells; 2) soak in 1mol / L HCl for more than 12 hours, then wash with distilled water to neutral to remove crab shells 3) Repeat the above steps 2-3 times, wash with distilled water until neutral, then use 0.5% NaClO 2 , glacial acetic acid to adjust the pH to 5.0, bleach in a 70°C water bath for 2 hours (preheat it first to reach 2.5 hours), and stir intermittently (if the bleaching is not complete, wash to neutral and then perform secondary bleaching); 4) After bleaching The sample was washed with distilled water until neutral, then pulverized with a juicer, weighe...

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Abstract

The invention belongs to the technical field of preparation of chitin nanofibers and relates to chitin deacetylase and chitin nanofiber dispersion liquid as well as a preparation method and application of the chitin nanofiber dispersion liquid. The chitin deacetylase is prepared from a penicillium oxalicum 3.5705 strain through purification, wherein the collection number of the penicillium oxalicum strain is CGMCC No. 3.5705. The preparation method of the chitin nanofiber dispersion liquid comprises the following steps: Performing partial deacetylation modification on chitin by using the chitin deacetylase, to obtain partial chitosan; treating the partial chitosan under a mechanical effect, to obtain the chitin nanofiber dispersion liquid. According to the chitin deacetylase and the chitinnanofiber dispersion liquid as well as the preparation method and application of the chitin nanofiber dispersion liquid provided by the invention, the chitin is subjected to partial deacetylation modification by a novel biological enzyme process instead of traditional concentrated base pyrolysis chemical treatment, and the reaction is mild, nontoxic and pollution-free; the preparation method is green and environmentally friendly, the yield of chitin nanofiber is increased, and efficient utilization of the chitin biomass resources is realized.

Description

technical field [0001] The invention belongs to the technical field of preparation of chitin nanofibers, and in particular relates to a chitin deacetylase, a chitin nanofiber dispersion, and a preparation method and application thereof. Background technique [0002] Chitin-based polysaccharide-based bio-nanomaterials have strong advantages in biofunctional materials due to their non-toxic, renewable, and biodegradable properties. Further research as biomaterials is hot, and countries around the world have Start gradually and research in depth. Partial deacetylation reaction is a key step in the preparation of chitin nanofibers. At present, the preparation of chitin nanofibers is mostly based on the deacetylation of chitin by chemical methods, for example, the deacetylation of chitin by concentrated alkali pyrolysis. However, this method will lead to environmental pollution, waste of chemical reagents, high energy consumption in the production process, and the degree of dea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/80C08J3/02C08L5/08
CPCC12N9/80C12Y305/01041C08J3/02C08J2305/08
Inventor 范一民叶文博俞娟王志国
Owner NANJING FORESTRY UNIV
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