Method and device for detecting tumor cell in blood by using stimulated Raman spectrum

Abstract

The invention discloses a method for detecting a tumor cell in blood by using a stimulated Raman spectrum. The method comprises the following steps: creating a stimulated Raman spectrum database for chemical ingredients of related substances in various related tumor cells, testing stimulated Raman characteristic spectrum data of the characteristic chemical ingredients of various related tumor cells of a test sample, using an intrinsic molecular vibration Raman spectrum signal of the characteristic chemical ingredient of the tumor cell as a marker for stimulated Raman imaging, measuring the chemical substance of the test sample through a Bessel beam based stimulated Raman projection real-time imaging, acquiring information of the characteristic chemical ingredient of the tumor cell in the whole test sample, comparing the stimulated Raman data in the database, and determining whether the test sample contains certain types of tumor cells. The invention further provides a device for detecting the tumor cell in the blood by using the stimulated Raman spectrum. The circulating tumor cell in the blood is detected through the Bessel beam based stimulated Raman projection real-time imaging,and the different tumor cells are detected at high sensitivity.

Description

technical field

[0001] The invention belongs to the field of bioinformatics, and relates to a method for detecting tumor cells, in particular to a method and a device for detecting tumor cells in blood by using stimulated Raman spectroscopy. Background technique

[0002] Distant metastasis of malignant tumors is one of the main reasons for clinical treatment failure or recurrence of solid malignant tumors. The existence of circulating tumor cells is the root cause of distant metastasis of solid malignant tumors. Circulating tumor cells are tumor cells present in the blood with the antigenic and molecular genetic properties of the original tumor. Circulating tumor cells originate from primary tumors or metastases, and when they encounter the stromal environment of suitable organs and tissues, they will settle and grow here, that is, tumor metastasis occurs. However, most of the tumor cells entering the circulation will lose their activity, and only less than 0.01% can reach...

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