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Preparation method and application of sandwich type immunosensor for detecting stomach cancer tumor marker

A tumor marker and immunosensor technology, which is applied in the field of preparation of sandwich type immunosensors for detecting gastric cancer tumor markers, can solve the problems of cumbersome detection methods, high cost and high detection limit, and achieves good biocompatibility and low cost. , the effect of more active sites

Active Publication Date: 2018-11-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many detection methods for tumor markers, such as radioimmunoassay, immunoradiometric assay, chemiluminescence assay, time-resolved fluorescent immunoassay, etc. Therefore, it is of great significance to establish a fast, simple and sensitive detection method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. A preparation method for detecting gastric cancer tumor marker sandwich type immunosensor, characterized in that the preparation steps are as follows:

[0039] (1) Preparation of h-MoS 2 / Au NPs

[0040] Take 20 mg of (NH 4 ) 2 MoS 4 and 2mL of 1% HAuCl 4 4H 2 O was mixed and dissolved in 20 mL of ultrapure water, and ultrasonicated for 10 min; then, 100 µL of hydrazine hydrate solution was added, and the mixed liquid was ultrasonically dispersed for 30 min to disperse the mixed liquid evenly; the mixed liquid was transferred to a 50 mL polytetrafluoroethylene reactor and heated to 180 ℃, reacted for 8 h; cooled to room temperature, and centrifuged and washed twice with ultrapure water and ethanol; the product was dissolved in 3 mL ultrapure water again, and dried in a lyophilizer to obtain h-MoS 2 / Au NPs;

[0041] (2) Preparation of Cu 2 O@TiO 2 / PtCu-Ab 2 Detection antibody incubation solution

[0042] Add 8 mL, 2.0 mol / L NaOH solution dropwise to 100 m...

Embodiment 2

[0060] 1. A preparation method for detecting gastric cancer tumor marker sandwich type immunosensor, characterized in that the preparation steps are as follows:

[0061] (1) Preparation of h-MoS 2 / Au NPs

[0062] Take 22 mg of (NH 4 ) 2 MoS 4 and 2mL of 1% HAuCl 4 4H 2 O was mixed and dissolved in 20 mL of ultrapure water, and ultrasonicated for 10 min; then, 100 µL of hydrazine hydrate solution was added, and the mixed liquid was ultrasonically dispersed for 30 min; the mixed liquid was transferred to a 50 mL polytetrafluoroethylene reactor , heated to 200 °C, and reacted for 10 h; cooled to room temperature, washed with ultrapure water and ethanol for 3 times; the product was dissolved in 3 mL ultrapure water again, and dried by a lyophilizer to obtain h-MoS 2 / Au NPs;

[0063] (2) Preparation of Cu 2 O@TiO 2 / PtCu-Ab 2 Detection antibody incubation solution

[0064] Add 10 mL, 2.0 mol / L NaOH solution dropwise to 100 mL, 0.01 mol / L CuCl containing 3.33 g polyvinyl...

Embodiment 3

[0082] 1. A preparation method for detecting gastric cancer tumor marker sandwich type immunosensor, characterized in that the preparation steps are as follows:

[0083] (1) Preparation of h-MoS 2 / Au NPs

[0084] Take 24 mg of (NH 4 ) 2 MoS 4 and 2mL of 1% HAuCl 4 4H 2 O was mixed and dissolved in 20 mL of ultrapure water, and sonicated for 10 min; then, 100 µL of hydrazine hydrate solution was added, and the mixture was sonicated for 30 min to disperse the mixture evenly; Heated to 220 °C and reacted for 12 hours; cooled to room temperature, centrifuged and washed four times with ultrapure water and ethanol; the product was dissolved in 3 mL ultrapure water again, and dried by a lyophilizer to obtain h-MoS 2 / Au NPs;

[0085] (2) Preparation of Cu 2 O@TiO 2 / PtCu-Ab 2 Detection antibody incubation solution

[0086] Add 12 mL, 2.0 mol / L NaOH solution dropwise to 100 mL, 0.01 mol / L CuCl containing 3.33 g polyvinylpyrrolidone 2 solution, fully stirred for 0.5 h; con...

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Abstract

The invention relates to a preparation method and application of a sandwich type immunosensor for detecting a stomach cancer tumor marker, and belongs to the field of novel nano functional materials,immunoassay and biological sensors. Three-dimensional porous molybdenum disulfide loading gold nano particle h-MoS2 / Au NPs is prepared to serve as a substrate material to modify a bare glassy carbon electrode, the solid supporting amount of the tumor marker to capture antibody is improved, and the electron transferring rate of electrode surface is effectively accelerated; an octahedral cuprous oxide / titanium dioxide core-shell structure loading mesoporous platinoid nano particle Cu2O@TiO2 / PtCu composite nano material is prepared to immobilize and detect the antibody, and the simple, rapid andsensitive sandwich type electrochemical immunosensor is prepared by utilizing the excellent electrochemical catalytic property of the composite nano material to hydrogen peroxide H2O2, and the immunosensor is used for detecting related tumor markers of stomach cancer.

Description

Technical field [0001] The invention belongs to the technical fields of nanofunctional materials, immunoanalysis and biosensing, and specifically relates to a preparation method and application of a sandwich immunosensor for detecting gastric cancer tumor markers. Background technique [0002] Gastric cancer has become one of the common malignant tumors in my country. There are 405,000 new gastric cancer cases in China every year, accounting for 42.5% of the new cases in the world. The incidence rate ranks first among digestive system tumors. Experts say: Gastric cancer is preventable and treatable. , as long as early detection and early treatment are achieved, the cure rate can reach 90%; therefore, early diagnosis has important clinical significance for the prevention and treatment of gastric cancer. [0003] CA72-4 is currently one of the best tumor markers for diagnosing gastric cancer. It has high specificity for gastric cancer, and its sensitivity can reach 28-80%. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3278
Inventor 李法瀛魏琴冯金慧曹伟范大伟吴丹庞雪辉马洪敏
Owner UNIV OF JINAN
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