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Culture method of high-enzyme activity fermentation broth and method for extracting acid-resistant alpha-amylase

A culture method, fermentation culture technology, applied in the directions of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of affecting the economic benefits of product promotion, high consumption, low enzyme activity, etc., to achieve easy control of density and dissolved oxygen, reduce Production cost, good acid resistance

Inactive Publication Date: 2018-11-16
ANHUI NEW SIMON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the acid-resistant α-amylases researched and produced by domestic scientific research institutes and production enterprises generally have low enzyme activity, fine requirements for raw and auxiliary materials, and high consumption. The relatively high enzyme activity only reaches 25000U / mL, which seriously affects the Promotion of products and economic benefits of enterprises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides a method for cultivating a fermented liquid with high enzyme activity, including slant culture, mother liquor culture, primary seed culture and fermentation culture,

[0041] The raw material components of the culture medium of the first-order seed culture include:

[0042] Corn steep liquor dry powder 12‰, soybean meal 18‰, DF-103 1.5‰, magnesium sulfate 3‰, potassium dihydrogen phosphate 4‰, dipotassium hydrogen phosphate 30‰, ferrous sulfate 0.015‰, manganese chloride 0.002‰, zinc chloride 0.005‰, glucose 15‰, and the balance is water.

[0043] The method for said primary seed cultivation, comprising the steps of:

[0044]Fully mix the raw materials except glucose, adjust the pH to 7.3, sterilize at 121°C for 30 minutes, and cool to 42°C to obtain a sterilized and cooled mixture; sterilize the glucose at 121°C for 30 minutes, cool to 42°C, Obtain sterilized and cooled glucose; add the sterilized and cooled glucose to the sterilized and cool...

Embodiment 2

[0064] This embodiment provides a method for cultivating a fermented liquid with high enzyme activity, including slant culture, mother liquor culture, primary seed culture and fermentation culture,

[0065] The raw material components of the culture medium of the first-order seed culture include:

[0066] Corn steep liquor dry powder 20‰, soybean meal 25‰, DF-103 3‰, magnesium sulfate 5‰, potassium dihydrogen phosphate 9‰, dipotassium hydrogen phosphate 50‰, ferrous sulfate 0.025‰, manganese chloride 0.006‰, zinc chloride 0.008‰, glucose 25‰, and the balance is water.

[0067] The method for said primary seed cultivation, comprising the steps of:

[0068] Fully mix the raw materials except glucose, adjust the pH to 7.6, sterilize at 123°C for 50 minutes, and cool to 42°C to obtain a sterilized and cooled mixture; sterilize the glucose at 123°C for 50 minutes, cool to 42°C, Obtain sterilized and cooled glucose; add the sterilized and cooled glucose to the sterilized and coole...

Embodiment 3

[0088] This embodiment provides a method for cultivating a fermented liquid with high enzyme activity, including slant culture, mother liquor culture, primary seed culture and fermentation culture,

[0089] The raw material components of the culture medium of the first-order seed culture include:

[0090] Corn steep liquor dry powder 15‰, soybean meal 20‰, DF-103 2‰, magnesium sulfate 3.5‰, potassium dihydrogen phosphate 5‰, dipotassium hydrogen phosphate 35‰, ferrous sulfate 0.02‰, manganese chloride 0.003‰, zinc chloride 0.006‰, glucose 18‰, and the balance is water.

[0091] The method for said primary seed cultivation, comprising the steps of:

[0092]Fully mix the raw materials except glucose, adjust the pH to 7.6, sterilize at 121°C for 30 minutes, and cool to 42°C to obtain a sterilized and cooled mixture; sterilize the glucose at 121°C for 30 minutes, cool to 42°C, Obtain sterilized and cooled glucose; add the sterilized and cooled glucose to the sterilized and coole...

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PUM

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Abstract

The invention relates to a culture method of a high-enzyme activity fermentation broth and a method for extracting an acid-resistant alpha-amylase. In the culture method of the high-enzyme activity fermentation broth, by optimizing a proportion of a culture medium of primary seed culture and fermentation culture, rising a culture temperature, controlling dissolved oxygen and adding a supplementingtechnology, the bacterial strain growth and metabolism demand is fully met, and a microenvironment more beneficial to bacterial strain growth is created; for the acid-resistant alpha-amylase extracted from the finally prepared high-enzyme activity fermentation broth, the enzyme activity reaches 75,000-80,000U / mL and is multiplied compared with the ordinary enzyme activity of 25,000U / mL, the acidresistance is better, a pH application range is 4.5-6, and the production cost of fermentation raw and auxiliary materials is greatly lowered at the same time, so that popularization and application of enzyme have a wider space and has very large social and economic values.

Description

technical field [0001] The invention relates to a production method of a biological enzyme preparation, in particular to a method for cultivating a fermented liquid with high enzyme activity and a method for extracting acid-resistant alpha-amylase. Background technique [0002] Acid-resistant α-amylase refers to an enzyme that can hydrolyze starch under acidic conditions. It can liquefy starch at a lower pH and maintain high activity of the enzyme. The remarkable acid resistance of acid-resistant α-amylase has great application space and potential. It is widely used in food, fermentation, textile, feed, pharmaceutical and other industries, which can significantly increase the yield and reduce energy consumption. [0003] At present, the acid-resistant α-amylases researched and produced by domestic scientific research institutes and production enterprises generally have low enzyme activity, fine requirements for raw and auxiliary materials, and high consumption. The relativel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/26
CPCC12N9/2414
Inventor 荣东徐冲卢一飞褚伟立张晓华程福宝
Owner ANHUI NEW SIMON BIOTECH CO LTD