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Rapid in-vitro propagation method of lily

A lily and in vitro technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as pollution, increased workload, bulbs with bacteria, etc., to reduce cross-contamination rate, shorten regeneration cycle, improve induction rate effect

Inactive Publication Date: 2018-11-20
沿河后花园农业观光旅游综合开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lily bulbs infected by the virus will grow into plants as the bulb buds germinate, and the virus will replicate and reproduce in the lily plant, resulting in serious infection of the bulbs. Often, the bulbs are not thoroughly sterilized during tissue culture, resulting in The phenomenon of repeated pollution is serious, increasing the workload, and the regeneration efficiency of explants using scales is not very ideal

Method used

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  • Rapid in-vitro propagation method of lily
  • Rapid in-vitro propagation method of lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select a good variety of Lily tomentosa and plant it in a well-drained, loose and fertile land around October of that year. In mid-May of the following year, cut off a 0.5cm thick stem tip with axillary buds of 2cm, and use 70% alcohol, 1.0% Sodium hypochlorite disinfectant and lily explant volume are 1:7 solution and sterile water disinfection;

[0025] The sterilized lily shoot tips were used as explants for bud induction culture, at a mass concentration of 9mg L -1 Naphthalene acetic acid aqueous solution and / or indole butyric acid aqueous solution soaked the base of lily for 22h, after induction treatment, put it flat on MS solid medium to induce growth of adventitious buds, MS solid medium is MS+BA 2.0mg / L+NAA 1.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L; induction treatment conditions: temperature 15°C, light: 20 light periods / 4 dark periods. The germination rate can reach 70%, and the average number of buds induced by each explant is about 11.

[0026] After 40 d...

Embodiment 2

[0029] Select good varieties of lily lily and plant them in a well-drained, loose and fertile land around October of that year. In mid-May of the following year, cut the stem tip with a stem diameter of 0.8 cm and axillary buds of 6 cm, and use 70% alcohol, 1.0 % sodium hypochlorite disinfectant and lily explant volume are 1:9 solution and sterile water disinfection;

[0030] Use the sterilized lily stem tips as explants for bud induction culture, soak the lily base for 23 hours in an aqueous solution of naphthaleneacetic acid and / or indolebutyric acid with a mass concentration of 10 mg·L1, and place them flat on MS The induction growth of adventitious buds is carried out on solid medium, MS solid medium is MS+BA 2.0mg / L+NAA 1.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L; the conditions of induction treatment are: Temperature 15°C, light: 20 light periods / 4 dark periods. The germination rate can reach 81%, and the average number of buds induced by each explant is about 20.

[003...

Embodiment 3

[0034] Select excellent Sichuan lily varieties and plant them in well-drained, loose soil and fertile plots around October of that year. In mid-May of the following year, cut off stem tips with 1 cm thick stems and 8 cm axillary buds, and use 70% alcohol and 1.0% sodium hypochlorite. Disinfectant and lily explant volume are 1:10 solution and sterile water disinfection;

[0035] The sterilized lily shoot tips were used as explants for bud induction culture, at a mass concentration of 11mg L -1 Naphthalene acetic acid aqueous solution and / or indole butyric acid aqueous solution soak the base of lily for 24 hours, after induction treatment, put it flat on MS solid medium to induce growth of adventitious buds, MS solid medium is MS+BA 2.0mg / L+NAA 1.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L; induction treatment conditions: temperature 15°C, light: 20 light periods / 4 dark periods. The germination rate can reach 73%, and the average number of buds induced by each explant is about 13....

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Abstract

The invention relates to the technical field of agricultural rapid propagation, and specifically relates to a rapid in-vitro propagation method of lily. The method is characterized in that tender stemtips with axillary buds of the lily are treated as explants and can directly regenerate a large number of adventitious buds; the culture medium quality and the photophase are adjusted, so that new seedlings can grow; compared with the method for performing tissue culture through scales as explants, the method has the advantages that the regeneration rate is high; the cross contaminate rate of virus is decreased; in addition, the inductivity of the stem tips of the lily is increased; and the regeneration period is reduced.

Description

technical field [0001] The invention relates to the technical field of agricultural rapid propagation and seedling cultivation, in particular to a lily rapid propagation method in vitro. Background technique [0002] Lily is a perennial herb of the genus Lilium in the family Liliaceae. It is not only a world-renowned ornamental flower, but also has edible and medicinal value. Traditional lily propagation methods have disadvantages such as small reproduction coefficient and easy degeneration of multi-generation breeding, which have a certain impact on the quantity and quality of lily production. The application of tissue culture technology can overcome the above shortcomings and increase the propagation speed, which is an effective propagation method in future production. [0003] Lily bulbs are the most commonly used tissue culture materials for rapid propagation. Although it is relatively convenient to use bulbs as explants for regeneration, since lilies grow in open condi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄霞
Owner 沿河后花园农业观光旅游综合开发有限公司
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