Method for extracting coriolus versicolor polysaccharides from coriolus versicolor

A technology of Yunzhi polysaccharides and crude extracts, which is applied in fermentation and other directions, can solve problems such as unsatisfactory extraction effects, and achieve the effects of short processing time, simple operation steps, and solvent saving

Inactive Publication Date: 2018-11-20
GUANGXI XIUMEI ZHUANGXIANG ENERGY ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, new extraction techniques and methods such as ultrasound or microblog-assisted extraction, ultra-high pressure, and enzymatic methods have emerged, but the extraction effects of these methods are not ideal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting polysaccharides from versicolor versicolor, comprising the following steps,

[0029] S1. Degreasing: dry and coarsely crush the fruiting body of Versicolor versicolor; add 8 times the weight of ethanol, the volume fraction of which is 85%, reflux extraction at 75°C for degreasing to obtain a crude extract. The fruiting bodies of Versicolor versicolor were roughly crushed into 50 meshes.

[0030] S2. Infiltration: the crude extract is dried and broken into 500 meshes. Then add 6 times the weight of water and soak at 60°C for 2 hours to obtain a pretreatment solution.

[0031] S3. Enzyme treatment: adjust the pH of the pretreatment solution to 7, then add a complex enzyme of 5% of the mass of Versicolor versicolor fruiting body, treat at 40-55° C. for 1-3 hours, and then inactivate the enzyme to obtain an extract. The enzyme inactivation is treated at 90° C. for 15 minutes.

[0032] The compound enzyme comprises cellulase, hemicellulase, pectina...

Embodiment 2

[0037] A method for extracting polysaccharides from versicolor versicolor, comprising the following steps,

[0038] S1. Degreasing: drying and coarsely crushing the fruiting body of Versicolor versicolor; adding 5 times the weight of ethanol, the volume fraction of which is 90%, reflux extraction at 80° C. for degreasing to obtain a crude extract. The fruiting bodies of Versicolor versicolor were roughly crushed into 40 meshes.

[0039] S2. Infiltration: the crude extract is dried and broken into 600 meshes. Then add 5 times the weight of water and soak at 70°C for 1 hour to obtain a pretreatment solution.

[0040] S3. Enzyme treatment: adjust the pH of the pretreatment solution to 6, then add a compound enzyme of 3% of the mass of Versicolor versicolor fruiting bodies, treat at 55° C. for 1 hour, and then inactivate the enzyme to obtain an extract. The enzyme inactivation is treated at 85° C. for 20 minutes.

[0041] The compound enzyme comprises cellulase, hemicellulase, ...

Embodiment 3

[0046] A method for extracting polysaccharides from versicolor versicolor, comprising the following steps,

[0047]S1. Degreasing: drying and coarsely crushing the fruiting body of Versicolor versicolor; adding 10 times the weight of ethanol, the volume fraction of which is 80%, reflux extraction at 75°C for degreasing to obtain a crude extract. The fruiting bodies of Versicolor versicolor were roughly crushed into 50 meshes.

[0048] S2. Infiltration: the crude extract is dried and broken into 400 meshes. Then add 5 times the weight of water and soak for 3 hours at 50°C to obtain a pretreatment solution.

[0049] S3. Enzyme treatment: adjust the pH of the pretreatment solution to 8, then add a complex enzyme of 8% of the mass of Versicolor versicolor fruiting bodies, treat at 40° C. for 3 hours, and then inactivate the enzyme to obtain an extract. The enzyme inactivation is treated at 95° C. for 10 minutes.

[0050] The compound enzyme comprises cellulase, hemicellulase, p...

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PUM

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Abstract

The invention provides a method for extracting coriolus versicolor polysaccharides from coriolus versicolor and relates to the technical field of bio-pharmaceuticals. The extraction method comprises the following steps: degreasing, infiltrating, performing enzyme treatment, performing ultrasonic extraction, and performing secondary extraction and alcohol precipitation. The extraction method adoptsthe synergistic effects of degreasing, wall-breaking treatment, enzyme treatment and ultrasonic- and microwave-assisted methods, and has the advantages of being simple in operating steps, solvent-saving, high in product yield, short in treatment time and the like.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for extracting polysaccharides from versicolor versicolor. Background technique [0002] Coriolus versicolor (Coriolus versicolor), also known as Coriolus versicolor, Melaleuca versicolor, etc., belongs to Fungi, Basidiomycotina, Polyporaceae, and Coriolus genus, and is a widely distributed large wood species. Coriolus versicolor is sweet and mild in taste and slightly cold in nature. It has the functions of invigorating the spleen and dampness, relieving cough and asthma, clearing away heat and detoxification, and anti-tumor. It is a traditional precious medicinal material in my country. Yunzhi polysaccharide is the main active ingredient of Yunzhi, which can activate immune cells, improve the body's immune function, anti-tumor, lower blood fat and antibacterial. In addition, Yunzhi polysaccharide has significant effects on learning and memory impairment cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/14C12P19/04
CPCC08B37/0024C08B37/0003C12P19/04C12P19/14
Inventor 谢英健
Owner GUANGXI XIUMEI ZHUANGXIANG ENERGY ENVIRONMENTAL PROTECTION CO LTD
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