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Method for increasing fucoxanthin content in fermented liquid of heterotrophic cultured Nitzschia smoothis by using light

A technology of heterotrophic culture and fucoxanthin, which is applied in the field of post-treatment of fermentation broth, can solve the problems that have not explored the influence of fucoxanthin accumulation on the heterotrophic culture characteristics of algae, and achieve the effect of increasing the content of fucoxanthin

Active Publication Date: 2022-02-08
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Heui-Chun et al. studied the effect of different light qualities (blue light, green light, red light and white light) on the accumulation of fucoxanthin in the marine diatom Chaetoceroscalcitrans, and found that blue light stimulated the accumulation of fucoxanthin, while red light stimulated the accumulation of fucoxanthin. Light inhibits the accumulation of fucoxanthin, but the algae used in this study is Chaetoceros, and it was cultivated by continuous irradiation with monochromatic light, and the heterotrophic culture characteristics of the algae and the effect on the accumulation of fucoxanthin in the cells after fermentation were not explored ( Bulletin of the Korean Society of Fisheries Technology, 2014, 50:447-454)
The applicant found that the effect of light quality on the accumulation of fucoxanthin in Nitzschia smoothis has not been reported so far

Method used

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  • Method for increasing fucoxanthin content in fermented liquid of heterotrophic cultured Nitzschia smoothis by using light
  • Method for increasing fucoxanthin content in fermented liquid of heterotrophic cultured Nitzschia smoothis by using light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Light-induced increase of fucoxanthin content in Nitzbug cells after fermentation

[0042] Nitzschia laevis UTEX 2047 (purchased from the Microalgae Collection of the University of Texas at Austin, Culture Collection of Algae at The University of Texas Austin, UTEX for short) was selected as the production strain.

[0043] The process steps are as follows:

[0044] A, adopting the method for heterotrophic culture to prepare Nitzschia smoothis fermented liquid, the substratum of heterotrophic culture comprises following raw material component:

[0045] NaCl 10g / L-32g / L; NaCl 2 SiO 3 9H 2 O 30mg / L-700mg / L; MgSO 4 ·7H 2 O 1.09g / L-2.18g / L; CaCl 2 2H 2 O 0.1g / L-0.27g / L; KH 2 PO 4 0.031g / L-0.062g / L; K 2 HPO 4 0.00375g / L-0.0075g / L; FeCl 3 ·6H 2 O 0.291mg / L-0.582mg / L; MnCl 2 4H 2 O 0.025mg / L-0.246mg / L; ZnCl 2 0.031mg / L-0.311mg / L; CoCl 2 ·6H 2 O 0.0114mg / L-0.0228mg / L; Na 2 MoO 4 2H 2 O 0.012mg / L-0.024mg / L; H 3 BO 3 3.06g / L-30.56g / L; (NH 4 ) 6 MO 7 o ...

Embodiment 2

[0052] The difference between this example and Example 1 is that the alga species in this example is Nitzschialaevis CCMP559 (purchased from the National Center for Marine Algae and Microbiota, NCMA for short).

Embodiment 3

[0054] The difference between this example and Example 1 is that the algal species in this example is Nitzschialaevis CCMP 1092 (purchased from the National Center for Marine Algae and Microbiota, NCMA for short).

[0055] The effect analysis of embodiment 1-3:

[0056] Under the culture condition of mixed light (blue light: white light = 1:1), the content of fucoxanthin in Nitzschia smoothis was up to 1.19% (accounting for cell dry weight), which was 58.7% higher than that of heterotrophic fermentation without light.

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Abstract

The invention belongs to post-treatment of fermented liquid, in particular to a method for increasing the content of fucoxanthin in the fermented liquid of Nitzschia smoothis by using light. The method comprises the steps of preparing the fermented liquid of Nitzschia smoothis by means of heterotrophic culture; transferring the fermented liquid of Nitzschia smoothis into a photobioreactor under the irradiation of monochromatic light or mixed light; Steps; the present invention solves the technical problem of low fucoxanthin content in Nitzschia plastifolia fermented liquid prepared by heterotrophic culture existing in the prior art. The prepared dry algal powder of Nitzschia smoothis has a high content of fucoxanthin, can stably realize continuous industrial production, can control heavy metals, polychlorinated biphenyls and other marine common pollutants from the source of the culture medium, and is derived from larger seaweeds. Fucoxanthin is safer and so on.

Description

technical field [0001] The invention belongs to post-treatment of fermented liquid, in particular to a method for increasing the content of fucoxanthin in the fermented liquid of Nitzschia smoothis by using light. Background technique [0002] Fucoxanthin is a natural carotenoid, also known as fucoxanthin and fucoxanthin, which participates in the reaction of photosystem II in photosynthesis. Fucoxanthin has a unique allene structure and is an active molecule with strong antioxidant properties. It has been confirmed to have multiple functional activities in cells, animals and humans, including anti-obesity, anti-diabetes, anti-inflammation, anti- Cancer, anti-angiogenic and anti-malarial activities. Based on the above functions, fucoxanthin is a natural product with broad prospects for health food and drug development, and has huge market value. [0003] Currently on the market, fucoxanthin is mainly extracted from large seaweeds such as Undaria pinnatifida and Laminaria j...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/02C12R1/89
CPCC12P17/02
Inventor 刘宾陈峰卢雪
Owner PEKING UNIV
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