Antimildew agent and antimildew method for peanuts
A kind of antifungal agent and peanut technology, applied in biocides, biocides, plant growth regulators, etc., can solve the problems of poor inhibition of Aspergillus flavus, easy volatilization and dissipation of essential oils, unfavorable applications, etc., to reduce content, prevent Effects of mold contamination
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Embodiment 1
[0026] 1. Preparation of bacteria-containing plates
[0027] On the ultra-clean workbench, use a pipette gun to pipette the activated Aspergillus flavus liquid (the specific concentration is about 10 7 CFU / mL) 100 μL, inoculated in Zapei's medium, spread evenly to make a bacteria-containing plate; paste a sterilized filter paper (d=6mm) on the center of the bacteria-containing plate cover.
[0028] 2. Determination of the minimum inhibitory concentration of plant essential oils when used alone
[0029] Dilute cinnamon essential oil, fennel essential oil and oregano oil with Tween-80, draw 0.2 mL of each dilution solution under sterile conditions, add it to the filter paper in the center of the plate plate containing bacteria, seal it with a sealing tape, and incubate it upside down. Make the essential oil concentration in the air in the petri dish reach 5-150mg / L air series concentration respectively, incubate at 28°C for 5 days, take the lowest concentration of the sample th...
Embodiment 2
[0041] An antifungal agent, which is formed by mixing compound plant essential oil, solvent and stabilizer according to the weight ratio of 80:16:4;
[0042] The compound plant essential oil is composed of cinnamon essential oil, anise essential oil and oregano oil in a weight ratio of 20:10:10.
[0043] Described solvent is ethanol;
[0044] The stabilizer is ethylene glycol.
Embodiment 3
[0046] An antifungal agent, which is formed by mixing compound plant essential oil, solvent and stabilizer according to the weight ratio of 75:20:5;
[0047] The compound plant essential oil is composed of cinnamon essential oil, fennel essential oil and oregano oil in a weight ratio of 16.67:16.67:16.67.
[0048] Described solvent is ethanol;
[0049] The stabilizer is glycerol.
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