Method for improving activity of grape superoxide dismutase by salicylic acid and application
A technology of dismutase activity and superoxide, applied in application, fertilization method, fertilizer made of biological waste, etc., to achieve the effect of improving quality, delaying the speed of nutrient loss, and improving grape quality
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Embodiment 1
[0029] Embodiment one: the method that salicylic acid of the present invention improves grape superoxide dismutase activity
[0030] The invention provides a kind of method that salicylic acid improves grape superoxide dismutase activity, and described method comprises the steps:
[0031] (1) Add salicylic acid and Tween-20 into deionized water to form a salicylic acid aqueous solution with a concentration of 1.0 mmol / L, and the volume fraction of Tween-20 in the solution is 0.05%.
[0032] (2) Select fruit ears with consistent growth potential and good lighting, and spray grape ears in four stages: flowering stage, swelling stage (late July), color change stage (late August) and 2 days before harvest (mid-September). Apply the formulated salicylic acid treatment.
[0033] (3) Each stage of spraying is divided into several times to spray the fruit surface, spray evenly, spray about 200mL of liquid on each cluster of fruit ears, and dry it. After each stage of spraying, start ...
Embodiment 2
[0036] Embodiment two: the optimization test of salicylic acid concentration of the present invention
[0037] Three salicylic acid (SA) treatment concentrations were set in the experiment, which were 0.5mmoL / L (S1), 1.0mmoL / L (S2), and 2.0mmoL / L (S3), and each salicylic acid solution contained a volume fraction of 0.05 %Tween-20, with water as the control group.
[0038] Select ears with consistent growth potential and good light, and spray salicylic acid on grape ears during the flowering period, late July (expansion period), late August (color change period) and mid-September (2 days before harvesting) . Spray the fruit surface several times in each stage, and spray evenly. Spray about 200mL of liquid medicine on each cluster of fruit ears in each stage, and dry it. A single plant is 1 repetition, and the average value is taken after 3 repetitions. After normal harvesting and pre-cooling, the laboratory was inoculated with Botrytis cinerea, and left for 60 days to measure...
Embodiment 3
[0043] Embodiment three: the method that salicylic acid of the present invention improves grape superoxide dismutase activity
[0044] The use method of the method for improving the activity of grape superoxide dismutase by salicylic acid of the present invention, the drying temperature controlled by the temperature control device is 24°C-35°C, and the wind speed controlled by the temperature control device is 8.0m / s~10.7m / s s, the amount of irrigation after fertilization is 10m 3 / hm 2 -15m 3 / hm 2 , The drip irrigation pipe is 40cm-60cm away from the grape roots, and the fertilization distance is 20cm-40cm from the grape roots.
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