Method for rapidly cultivating purified strain of Dendrobium candidum with red leaves and red stems

Abstract

The invention provides a method for rapidly cultivating a purified strain of Dendrobium candidum with red leaves and red stems. The method for rapidly cultivating the purified strain of the Dendrobiumcandidum with red leaves and red stems comprises the following steps: (1) acquisition of a hybrid fruit with germplasm of red leaves and red stems; 2) aseptic seeding; (3) in-bottle induction of traits of red leaves and red stems; (4) rapid purification and propagation through in-bottle bud cutting of the Dendrobium candidum with red leaves and red stems; and (5) rooting culture of the Dendrobiumcandidum with red leaves and red stems. The method provided by the invention requires simple equipment, and has the effects of rapidly screening and purifying the strain of the Dendrobiu.m candidum with red leaves and red stems. Compared with conventional variety breeding for the Dendrobium candidum with red leaves and red stems, a variety breeding cycle can be shortened from 3-5 years to less than 2 years, genotypes of strain individuals are consistent, and the strain individuals can be applied immediately in large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for rapidly cultivating purified strains of red-leaved red-rod Dendrobium officinale. Background technique [0002] Dendrobium officinale ( Dendrobium officinale ), also known as black knot grass, is the genus Dendrobium of the family Orchidaceae ( Dendrobium ) Perennial epiphytic herb, which is a rare Chinese herbal medicine in my country. Red leaves and red stems are important commodity traits of Dendrobium candidum. The market price of red leaves and red stems is usually 1-2 times higher than that of green stems. There is also a big difference in polysaccharide components and content between the two. The commercial breeding method of Dendrobium candidum with red leaves and red stems is usually obtained by aseptic sowing of the seeds of Dendrobium candidum with red leaves and red stems on the mother plant. Due to the high degree of heterozygosity of Dendrobi...

AI Technical Summary

Problems solved by technology

The commercial breeding method of Dendrobium candidum with red leaves and red stems is usually obtained by aseptic sowing of the seeds of Dendrobium candidum with red leaves and red stems on the mother plant. Due to the high degree of heterozygosity of Dendrobium candidum, it is difficult to sow offspring no matter by selfing or hybridization. In order to obtain lines with consistent traits, resulting in significant differences among the seedlings produced by different individuals

Under conventional tissue culture conditions, it is difficult to fully express the traits of Dendrobium officinale with red leaves and red stems. It is necessary to select stems as explants for tissue culture and rapid propagation in an outdoor transplanting environment. Although this method can obtain offspring with consistent traits, However, the process from hybridization, transplanting, selection to rapid propagation often takes 3-5 years

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