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A kind of active flavonoid compound and its preparation method and application

A flavonoid compound, active technology, applied in the field of active flavonoid compound and its preparation, can solve the problems of no anti-cervical cancer and gastric cancer

Active Publication Date: 2022-02-18
HUNAN XINHUI PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Document CN104069348A discloses a Polygonatum extract and its preparation method and application. In this invention, Polygonatum is thermally extracted with ethanol, and then extracted with petroleum ether, ethyl acetate, and n-butanol. The obtained compound is mainly used for anti-diabetes and its In terms of complications, there is no effect against cervical cancer and gastric cancer

Method used

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  • A kind of active flavonoid compound and its preparation method and application
  • A kind of active flavonoid compound and its preparation method and application
  • A kind of active flavonoid compound and its preparation method and application

Examples

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Embodiment 1

[0027] (1) get 500g Polygonatum polyflora root raw material, after drying, through pulverization process, obtain Polygonatum powder after sieving, be 95% ethanol extraction with 2500ml volume concentration, the instrument used for extraction is DTQ-200 type multifunctional extraction tank, extracts 2 After times, the extract was obtained;

[0028] (2) the extract is extracted with 2500ml ethyl acetate, the ethyl acetate layer is taken, concentrated to obtain medicinal extract;

[0029] (3) Load the extract on a silica gel column, and perform gradient elution with 1600ml of petroleum ether-ethyl acetate with a volume ratio of 100:1 to obtain 12 fractions A-L with polarity ranging from small to large;

[0030] (4) The fraction K with higher polarity is eluted with 3000ml of n-hexane-acetone with a volume ratio of 4:1 at a constant constant rate, and then the resulting product is loaded on a Sephadex column, and the volume ratio is 1:1 The chloroform-methanol was eluted and furt...

Embodiment 2

[0035] (1) Get 500g Polygonatum polyflora root raw material, after drying, through pulverization process, obtain Polygonatum powder after sieving, be 95% ethanol extraction with 4000ml volume concentration, extract and use instrument to be DTQ-200 type multifunctional extraction tank, extract 3 After times, the extract was obtained;

[0036] (2) the extract is extracted with 2000ml ethyl acetate, the ethyl acetate layer is taken, concentrated to obtain extract;

[0037] (3) Load the extract on a silica gel column, and use 2000ml of petroleum ether-ethyl acetate with a volume ratio of 2:1 for gradient elution to obtain 12 fractions A-L with polarity ranging from small to large;

[0038] (4) The fraction K with higher polarity was eluted with 3000ml of n-hexane-acetone with a volume ratio of 5:1, and then the resulting product was loaded on a Sephadex column, and the volume ratio was 2:1. The chloroform-methanol was eluted and further purified to obtain compound I, and the puri...

Embodiment 3

[0041] (1) Get 500g Polygonatum polyflora root raw material, after drying, through pulverization process, obtain Polygonatum powder after sieving, be 95% ethanol extraction with 3000ml volume concentration, extract and use instrument to be DTQ-200 type multifunctional extraction tank, extract 4 After times, the extract was obtained;

[0042] (2) the extract is extracted with 3000ml ethyl acetate, the ethyl acetate layer is taken, concentrated to obtain extract;

[0043] (3) Load the extract on a silica gel column, and use 3000ml of petroleum ether-ethyl acetate with a volume ratio of 50:1 for gradient elution to obtain 12 fractions A-L with polarity ranging from small to large;

[0044] (4) The fraction K with higher polarity was eluted with 3000ml of n-hexane-acetone with a volume ratio of 3:1, and then the resulting product was loaded on a Sephadex column, and the volume ratio was 1:2. The chloroform-methanol was eluted and further purified to obtain compound I, and the pur...

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Abstract

The invention discloses an active flavonoid compound and its preparation method and application. The structural formula of the active flavonoid compound is as follows: the synthesis steps of the compound are as follows: take Polygonatum polyflora raw material and extract it with ethanol to obtain an extract; extract the extract Extract with ethyl acetate, take the ethyl acetate layer, concentrate to obtain the extract; use advanced separation and purification technology (thin layer chromatography, column chromatography, preparative and semi-preparative HPLC technology, etc.) to separate and purify the extract, adopt modern pop technique such as 1 H-NMR, 13 C-NMR, 2D-NMR (COSY, HSQC, HMBC) etc. complete the identification work and obtain active flavonoids. The invention has the functions of resisting cervical cancer, stomach cancer and resisting oxidation.

Description

technical field [0001] The invention relates to the technical field of medicine extraction, in particular to an active flavonoid compound and its preparation method and application. Background technique [0002] Polygonatum multiflora is derived from the dry rhizome of Polygonatum multiflora in the genus Polygonatum of Liliaceae, also known as Polygonatum multiflora. It is mainly produced in southern China, and Hunan Province has a long history of cultivating Polygonatum multiflora, and it is one of its authentic places. "Compendium of Materia Medica" states that it "obtains the essence of the earth, which is the best product for nourishing the middle palace". It has the effects of nourishing the kidney and essence, nourishing yin and moistening dryness. It is also an important component of dozens of compound nourishing medicines. At present, there is no report of extracting flavonoids with anti-cervical cancer, gastric cancer and good antioxidant properties from Polygonat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/22A61P35/00A61P39/06A23L33/105
CPCA61P35/00A61P39/06A23L33/105C07D311/22A23V2002/00
Inventor 王炜何述金曾婷彭彩云袁汉文龚力民石继连何承东
Owner HUNAN XINHUI PHARMA
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