Preparation method of biosensing film, biosensing film and monitoring device

Abstract

The application discloses a preparation method of a biosensing film, which comprises the steps of: after carrying out electrochemical activation modification on oxidordeuctase, carrying out cross-linking processing by using a chemical cross-linking agent; and coating the obtained product on the surface of an electrode to form the biosensing film, wherein the chemical cross-linking agent is glutaraldehyde or polyethylene glycol diglycidyl ether. The application further discloses a biosensing film prepared by the method and a monitoring device. The preparation method provided by the applicationor the biosensing film prepared by the preparation method and the monitoring device are stable and durable, can carry out repeated detection and are particularly applicable to a biosensing film used as a living body monitoring device.

Description

technical field [0001] The present application relates to the technical field of detection equipment, in particular to a preparation method of a biosensing film, a biosensing film and a monitoring device. Background technique [0002] An electrochemical biosensor is a device that is sensitive to biological substances and can convert its concentration into an electrical signal for detection. Electrochemical biosensors include selective biological substances such as oxidoreductases and antibodies that can recognize target substances, and electrodes and their accessories that convert the recognition signals into electrical signals. For example, when oxidoreductases are used as target recognition substances, the exchange of electrons between them and electrodes is an important step in biosensing devices. Typically, however, the redox active site of an oxidoreductase does not exchange electrons with the electrode, and electron transfer between the enzyme's active site and the el...

AI Technical Summary

Problems solved by technology

Typically, however, the redox active site of an oxidoreductase does not exchange electrons with the electrode, and electron transfer between the enzyme's active site and the electrode is the limiting factor for most biosensors.

In order to overcome the problem of low electron transfer efficiency, redox small molecular compounds with excellent electrochemical properties can be introduced into the biosensing membrane, and an electronic channel can be established between the redox enzyme and the electrode, so that the electron exchange can be carried out quickly.

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