Preparation method of biosensing film, biosensing film and monitoring device

A sensing membrane, biological technology, applied in biochemical equipment and methods, measuring devices, enzymes, etc., can solve problems such as low electron transfer efficiency

Active Publication Date: 2018-11-30
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Typically, however, the redox active site of an oxidoreductase does not exchange electrons with the electrode, and electron transfer between the enzyme's active site and the electrode is the limiting factor for most biosensors.
In order to overcome the problem of low electron transfer efficiency, redox small molecular compounds with excellent electrochemical properties can be introduced into the biosensing membrane, and an electronic channel can be established between the redox enzyme and the electrode, so that the electron exchange can be carried out quickly.

Method used

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  • Preparation method of biosensing film, biosensing film and monitoring device
  • Preparation method of biosensing film, biosensing film and monitoring device
  • Preparation method of biosensing film, biosensing film and monitoring device

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preparation example Construction

[0031] The invention provides a method for preparing a biosensing membrane. After electrochemically activating and modifying an oxidoreductase, it is crosslinked with a chemical crosslinking agent and coated on the surface of an electrode to form a biosensing membrane. The chemical cross-linking agent is glutaraldehyde or polyethylene glycol diglycidyl ether. In the preparation method provided by the present invention, by using glutaraldehyde or polyethylene glycol diglycidyl ether as a chemical cross-linking agent, the modified oxidoreductase is treated, and then coated on the surface of the electrode to form a biosensing membrane. The biosensing film formed by glutaraldehyde or polyethylene glycol diglycidyl ether cross-linking treatment is stable and durable, and can be detected many times, and is especially suitable for the biosensing film as a living body monitoring device.

[0032] Preferably, the oxidoreductase is any one or more of glucose oxidase, glucose dehydrogena...

Embodiment 1

[0053] Add 1-20mg / ml of Os(bpy) 2 (3-Aminopropylimidazole) Cl, and glucose oxidase in PBS buffer solution are fully mixed, then sequentially add 0.1-25mmol / L carbodiimide and 0.01-5mmol / L N-hydroxysuccinimide, fully After mixing, react at 4°C for 24h. Then, the oxidoreductase after the first modification is separated and purified by ultrafiltration bag dialysis to cut off the molecular weight of 5000-50000. Then add 1-20mg / ml of Os(bpy) to the purified oxidoreductase solution 2 (4-imidazole butyric acid) Cl, then add 0.1-25mmol / L carbodiimide and 0.01-5mmol / L N-hydroxysuccinimide in turn, mix well and react at 4°C for 24h, after the reaction, use ultrafiltration again Bag dialysis, cutting molecular weight 500-50000, to separate and purify the oxidoreductase after the second modification. After glucose oxidase is modified, its catalytic active center can directly exchange very fast electrons with the electrode (see attached figure 1 shown). Using spectrophotometric analys...

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Abstract

The application discloses a preparation method of a biosensing film, which comprises the steps of: after carrying out electrochemical activation modification on oxidordeuctase, carrying out cross-linking processing by using a chemical cross-linking agent; and coating the obtained product on the surface of an electrode to form the biosensing film, wherein the chemical cross-linking agent is glutaraldehyde or polyethylene glycol diglycidyl ether. The application further discloses a biosensing film prepared by the method and a monitoring device. The preparation method provided by the applicationor the biosensing film prepared by the preparation method and the monitoring device are stable and durable, can carry out repeated detection and are particularly applicable to a biosensing film used as a living body monitoring device.

Description

technical field [0001] The present application relates to the technical field of detection equipment, in particular to a preparation method of a biosensing film, a biosensing film and a monitoring device. Background technique [0002] An electrochemical biosensor is a device that is sensitive to biological substances and can convert its concentration into an electrical signal for detection. Electrochemical biosensors include selective biological substances such as oxidoreductases and antibodies that can recognize target substances, and electrodes and their accessories that convert the recognition signals into electrical signals. For example, when oxidoreductases are used as target recognition substances, the exchange of electrons between them and electrodes is an important step in biosensing devices. Typically, however, the redox active site of an oxidoreductase does not exchange electrons with the electrode, and electron transfer between the enzyme's active site and the el...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/31
CPCG01N27/31G01N27/3272G01N27/3277C12N9/0006G01N27/3271C12Y101/03004C12N9/0069
Inventor 高志强
Owner SINOCARE
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