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Silk fibroin-based nanodrug for targeted combined chemotherapy of breast cancer, and preparation method thereof

A silk fibroin and nano-drug technology, which can be used in drug combinations, medical preparations with inactive ingredients, inactive ingredients of polymer compounds, etc., can solve the problems of poor water solubility and low oral bioavailability, and achieve stable concentration, Effects of improving pharmacokinetics, excellent biodegradability

Inactive Publication Date: 2018-12-04
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Although CUR and 5-FU have many advantages, their applications are limited due to their low oral bioavailability and poor water solubility.

Method used

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  • Silk fibroin-based nanodrug for targeted combined chemotherapy of breast cancer, and preparation method thereof
  • Silk fibroin-based nanodrug for targeted combined chemotherapy of breast cancer, and preparation method thereof
  • Silk fibroin-based nanodrug for targeted combined chemotherapy of breast cancer, and preparation method thereof

Examples

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Embodiment 1

[0044] Example 1 is to prepare nano-drugs based on silk fibroin for targeted combined chemotherapy of breast cancer, including the following preparation steps:

[0045] (1) After silkworm cocoons are degummed with weak alkali solution, they are completely dissolved in lithium bromide solution, and regenerated silk fibroin is obtained after dialysis with deionized water;

[0046] (2) Dissolve regenerated silk fibroin in secondary water, hydrate overnight at room temperature to obtain hydrated regenerated silk fibroin;

[0047] (3) Weigh an appropriate amount of curcumin (Curcumin, CUR) and pentafluorouracil (5-Fluorouracil, 5-FU), and then completely dissolve them in methanol to obtain a methanol solution in which CUR and 5-FU are dissolved;

[0048] (4) Add the hydrated regenerated silk fibroin obtained in step (2) dropwise to the methanol solution dissolved in CUR and 5-FU obtained in step (3) under vortex conditions, and the solution becomes turbid at this time. Emulsion, t...

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Abstract

The invention discloses a silk fibroin-based nanodrug for targeted combined chemotherapy of breast cancer, and a preparation method thereof. The nanodrug loaded with curcumin (CUR) and 5-fluorouracil(5-FU) is prepared through a solvent volatilization process by using regenerated silk fibroin as a carrier, and the surface of the nanodrug is modified with a targeted molecule hyaluronic acid (HA). The above nanoparticle has a small hydrated particle size (198.0 + / - 1.8 nm), a good size uniformity (PDI: 0.097), a high drug loading capacity (CUR: 2.1%; 5-FU: 1.7%) and a good dispersibility, is notprone to agglomerate, and is convenient to construct multifunctional drug-loaded silk fibroin nanoparticles. The silk fibroin-based targeted nanodrug obtained in the invention can effectively inhibittumor cell proliferation and increase the concentration of the anticancer drug in the cells. The hyaluronic acid (HA) for modifying the surface enhances the therapeutic effect of single drugs on thebreast cancer and achieves synergistic effects in cancer treatment.

Description

technical field [0001] The invention belongs to the technical field of nano-drug carriers, and in particular relates to a preparation method, characterization and application of a silk fibroin-based nano-drug loaded with two anti-tumor drugs for breast cancer targeting combined chemotherapy. Background technique [0002] As we all know, cancer is now recognized as one of the most challenging and devastating diseases in the world, accounting for 13% of all deaths worldwide. What's more serious is that the incidence of cancer and the death rate of the population in China have continued to rise sharply in recent years. The National Cancer Center analyzed and released the latest "China Malignant Tumor Incidence and Death Analysis Report" based on the 2014 case data. There were about 3.804 million cases of malignant tumors and 2.296 million deaths. China accounts for about 21.8% and 27% of the new cases and deaths of malignant tumors in the world, respectively. Various therapeu...

Claims

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Application Information

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IPC IPC(8): A61K31/513A61K31/12A61K47/42A61K47/36A61P35/00
CPCA61K31/12A61K31/513A61K47/36A61K47/42A61P35/00A61K2300/00
Inventor 肖波黄亚梅汪敏
Owner SOUTHWEST UNIVERSITY
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