Bigeminal test strip for foot-and-mouth disease virus immune antibody evaluation and infection-immunity differential diagnosis

Abstract

The invention discloses a bigeminal test strip for foot-and-mouth disease virus immune antibody evaluation and infection-immunity differential diagnosis. The test strip includes a detection line 1 printed with a foot-and-mouth disease virus VP1 epitope polypeptide artificial antigen, and a detection line 2 printed with a foot-and-mouth disease virus non-structural protein epitope polypeptide artificial antigen. The artificial antigens prepared according to the invention have the advantages of easy availability, stable structure, purity up to 99%, low cost, rapid mass production, etc., thus solving the shortcomings of low expression level of traditional expression protein, difficult guarantee of a natural spatial structure, difficult renaturation, difficult removal of mycoprotein, influenceon the detection result specificity and the like in traditional expression protein, and also solving the risk of incomplete virus inactivation in use of inactivated viruses. The invention also performs pretreatment on a gold labelled pad, thus being more conducive to water absorption of the gold labelled pad and release of gold labelled protein, and effectively solving the problems of slow and incomplete release of gold labelled protein, affection to the test paper product detection accuracy, sensitivity, shelf life and the like in existing test paper.

Description

technical field [0001] The invention relates to a foot-and-mouth disease virus immune antibody evaluation and infection and immune differential diagnosis dual test strip, which belongs to the technical field of immune detection. Background technique [0002] Foot-and-mouth disease is a highly contagious disease of artiodactyls caused by the foot-and-mouth disease virus. Foot-and-mouth disease virus has polymorphism, variability, wide range of hosts, and strong infectivity, so once it occurs, it will be epidemic and explosive. What's more serious is that foot-and-mouth disease is a severe infectious disease, which is said to be extremely contagious. Strict isolation, blockade, prohibition of animal movement and listing of livestock products must be carried out for diseased animals and animals in the same group exposed to the pathogen. , leading to the suspension of the import and export trade of livestock products in the affected areas and even the affected countries, result...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages of low protein expression in the prior art, difficulty in ensuring the natural spatial structure, difficulty in renaturation, bacterial protein affecting the specificity of the test results, and the risk of incomplete virus inactivation of the inactivated virus, and existing test strips The existing gold standard protein is released slowly and incompletely, which affects the detection accuracy, sensitivity and shelf life of test paper products. The present invention combines the advantages and disadvantages of the existing ELISA method for the detection of foot-and-mouth disease and test strips, and uses artificial antigens prepared by polypeptide epitopes and Gold standard pad treatment method, invention of a foot-and-mouth disease virus immune antibody evaluation and infection and immune differential diagnosis dual test strip

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