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Black spore truffle inoculation agent and its treatment method and mycorrhizal cultivation method

A cultivation method and truffle fungus technology are applied in the field of intersection of plant and microorganism technology, which can solve the problems of difficulty in cultivating mycorrhizal seedlings, low infection rate of mycorrhizal plants, etc. Effect

Active Publication Date: 2020-11-24
永仁野森达菌业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: the cultivation of mycorrhizal seedlings of black spore truffles faces the problems of low mycorrhizal infection rate and great difficulty in cultivating mycorrhizal seedlings

Method used

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  • Black spore truffle inoculation agent and its treatment method and mycorrhizal cultivation method
  • Black spore truffle inoculation agent and its treatment method and mycorrhizal cultivation method
  • Black spore truffle inoculation agent and its treatment method and mycorrhizal cultivation method

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Effect test

Embodiment 1

[0045] Embodiment 1 carries out the cultivation of American pecan mycorrhizal seedlings with the inventive method, specifically as follows:

[0046] 1. Preparation and incubation of black spore truffle inoculant

[0047] The preparation of the black spore truffle inoculum is prepared according to the mass fraction with reference to the quality of the black spore truffle fruiting body. The finished bacterial agent is first placed in a -80°C ultra-low temperature refrigerator to preserve its vitality, and then placed in a -20°C refrigerator for incubation. Incubate in a refrigerator at 4°C to promote the germination of truffle spores.

[0048] a. Species identification, disinfection and sterilization. In the season when the black spore truffle is fully mature, choose a fresh truffle fruiting body with a large, strong aroma and very high maturity, observe the maturity of the spores through an optical microscope, and use DNA molecular identification technology to identify 100% of...

Embodiment 2

[0072] Embodiment 2 carries out the cultivation of Chinese chestnut mycorrhizal seedling with the inventive method, specifically as follows:

[0073] 1. Preparation of black spore truffle inoculant and temperature gradient storage

[0074] The preparation of the black spore truffle inoculum is prepared according to the mass fraction with reference to the quality of the black spore truffle fruiting body. The finished bacterial agent is first placed in a -80°C ultra-low temperature refrigerator to preserve its vitality, and then placed in a -20°C refrigerator for incubation. Incubate in a refrigerator at 4°C to promote the germination of truffle spores.

[0075] a. Species identification, disinfection and sterilization. In the season when the black spore truffle is fully mature, choose a fresh truffle fruiting body with a large, strong aroma and very high maturity, observe the maturity of the spores through an optical microscope, and use DNA molecular identification technology ...

Embodiment 3

[0099] Embodiment 3 carries out the cultivation of Huashan pine mycorrhizal seedling with the inventive method, specifically as follows:

[0100] 1. Preparation and incubation of black spore truffle inoculant

[0101] The preparation of the black spore truffle inoculum is prepared according to the mass fraction with reference to the quality of the black spore truffle fruiting body. The finished bacterial agent is first placed in a -80°C ultra-low temperature refrigerator to preserve its vitality, and then placed in a -20°C refrigerator for incubation. Incubate in a refrigerator at 4°C to promote the germination of truffle spores.

[0102] a. Species identification, disinfection and sterilization. In the season when the black spore truffle is fully mature, choose a fresh truffle fruiting body with a large, strong aroma and very high maturity, observe the maturity of the spores through an optical microscope, and use DNA molecular identification technology to identify 100% of th...

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Abstract

The invention discloses a tuber melanosporum inoculant agent, a treatment method thereof and a cultivation method of mycorrhiza. The tuber melanosporum inoculant agent is prepared from, by weight, 10-20 parts of tuber melanosporum sporocarp, 2-4 parts of agricultural and forest water retaining agent, 0.008-0.015 part of gibberellin, 0.001-0.002 part of forchlorfenuron, 50-80 parts of vermiculite powder and 10-15 parts of limestone powder. The treatment method of the tuber melanosporum inoculant agent comprises the steps that the tuber melanosporum inoculant agent is contained in a container, firstly liquid nitrogen is used for performing spraying freezing for 2-5 minutes, the container is placed at -80 DEG C to be stored for 15-20 days, and placed at -20 DEG C to perform incubation for 10-15 days, and finally placed at 4 DEG C to perform incubation for 25-40 days, and then inoculation is performed on an aseptic host seedling. The tuber melanosporum inoculant agent, the treatment methodthereof and the cultivation method of the mycorrhiza can improve the impregnation rate and root system activity of the mycorrhiza of the tuber melanosporum.

Description

technical field [0001] The invention belongs to the interdisciplinary field of plant and microorganism technology, and specifically relates to a black spore truffle inoculum agent, a treatment method thereof, and a method for cultivating mycorrhizae. Background technique [0002] Truffles, also known as truffles, truffles, sycamores, pig arches, etc., have a unique aroma and high economic value. Europe, North America, and Southeast Asia are the main distribution areas of truffles, and they are known as "kitchens" in Europe. "Diamonds in" and "Food for God". Black spore truffle (Tuber melanosporum) is originally produced in France, Italy, Spain and other places. It is a precious mycorrhizal edible fungus containing androsterone and its precursor. The distribution of this truffle is not produced or found in my country. The price of black spore truffles in the international market is very expensive, reaching RMB 8,000-15,000 per 500g, and has a high international reputation. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/10A01G18/20
CPCA01G18/10A01G18/20
Inventor 李小林叶雷邹捷亢宗静王迪何晓兰陈影苗人云谭伟王勇姜邻彭卫红黄忠乾甘炳成张波谭昊
Owner 永仁野森达菌业有限公司
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