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A strain of Bacillus amyloliquefaciens yh-20 with broad-spectrum disease resistance and growth-promoting effect and its application

A technology of YH-20 and amylolytic spores, applied in the field of microorganisms, can solve the problems of ecological balance destruction, soil compaction, pesticide pollution, etc., and achieve the effects of promoting growth, protecting the ecological environment, and good antagonistic effects

Active Publication Date: 2019-10-29
NANJING FORESTRY UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chemical control is an important means of preventing and controlling forestry pests and diseases. my country is the world's largest user of pesticides and chemical fertilizers, but the extensive use of pesticides and chemical fertilizers has caused a series of problems such as environmental pollution, soil compaction, and ecological balance damage. With the deterioration of the environment, the problem of pesticide pollution has attracted more and more attention, so it is particularly important to find a method that is environmentally friendly and has a good control effect.

Method used

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  • A strain of Bacillus amyloliquefaciens yh-20 with broad-spectrum disease resistance and growth-promoting effect and its application
  • A strain of Bacillus amyloliquefaciens yh-20 with broad-spectrum disease resistance and growth-promoting effect and its application
  • A strain of Bacillus amyloliquefaciens yh-20 with broad-spectrum disease resistance and growth-promoting effect and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Streak and activate Bacillus amyloliquefaciens YH-20 on LB medium, culture in an incubator at 28°C for 24 hours, pick a single colony in a 1L Erlenmeyer flask with 500mL of LB liquid medium, culture at 180r / min, shake at 28°C for 72h, Obtain a fermented broth. Centrifuge the fermentation broth at 4°C and 10,000 r / min for 10 minutes to obtain a supernatant, which is filtered with a 0.22 μm microporous filter to obtain a sterile fermentation broth.

[0025] In this example, 10 kinds of plant pathogenic bacteria (all preserved in the Forest Pathology Laboratory of Nanjing Forestry University, which are available to the public upon request) are used as target bacteria. The ten kinds of plant pathogenic bacteria are: Fusicoccus aesculi and wheat scab (Gibberella zeae), Dothierella gregaria, Fusarium circinatum, Pestalotiopsis vesicolor, Guignardia camelliae, Colletotrichum sp), Alternaria sp, Sphaeropsis sapinea.

[0026] The inhibitory rate of Bacillus amyloliquefaciens Y...

Embodiment 2

[0031] A single colony of Bacillus amyloliquefaciens YH-20 was picked and placed in a 1L Erlenmeyer flask with 500mL of LB liquid medium, shaken at 180r / min at 28°C for 72h to obtain a fermentation broth. Filter the fermented liquid with a microporous filter of 0.22pm to obtain YH-20 aseptic fermented liquid, select 30 plump tomato seeds, use YH-20 aseptic fermented liquid and its different dilutions (10, 50, 100 , 200, 500, 1000 times) sterile water dilution solution and LB culture solution (blank control, CK) of the same dilution factor to soak the seeds in 10 mL each, and after 24 hours, put the seeds in a petri dish containing wet filter paper sheets, each Treat 30 seeds, establish 3 repetitions, measure and record the root length of seeds after 96h, the results are shown in Table 2, the results show that the root length of tomato seeds soaked in 50× fermentation broth has the highest increase rate, reaching 78.45%. So the fermentation broth of Bacillus amyloliquefaciens Y...

Embodiment 3

[0036] Streak and activate Bacillus amyloliquefaciens YH-20 on the LB plate, put it in a 28°C incubator and cultivate it for 24 hours, pick a single colony and place it in a shaker flask containing 30mL of LB medium, and set the temperature at 28°C, 180r / min Shake culture, count by hemocytometer, the bacterial concentration in the culture solution to be shaken is 10 8 cfu / mL, the fermentation broth was centrifuged at 10,000r / min to obtain the bacteria, and resuspended with the same volume of sterile water to obtain a concentration of 10 8 cfu / mL bacterial suspension, ready for use. The preparation method of aseptic fermented liquid is the same as embodiment 2.

[0037]The sterilized seeds were sown in the sowing tray, and after 30 days of germination, the original plant height was measured, and bacteria were applied. Using the culture medium as a control, the same volume of 50 mL of sterile fermentation broth, fermentation broth and bacterial suspension were applied respecti...

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Abstract

The present invention discloses a strain of Bacillus amyloliquefaciens YH-20 with broad-spectrum disease resistance and growth-promoting effect and its application. The strain is named Bacillus amyloliquefaciens (Bacillus. The collection center, the deposit number is CCTCC NO: M2018361, the deposit address is Wuhan University, Wuhan, China, and the deposit date is June 11, 2018. The bacillus amyloliquefaciens YH-20 of the present invention has good antagonistic effect on all 10 kinds of plant pathogenic fungi, has no harm to the plants themselves, and can obviously promote the growth of plants. Utilizing the bacillus amyloliquefaciens strain YH-20 provided by the invention can replace part of the application of pesticides and chemical fertilizers. It can effectively protect the ecological environment and protect the production of forestry trees.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Bacillus amyloliquefaciens YH-20 with broad-spectrum disease-resistant and growth-promoting effects and an application thereof. Background technique [0002] Chemical control is an important means of preventing and controlling forestry pests and diseases. my country is the world's largest user of pesticides and chemical fertilizers, but the extensive use of pesticides and chemical fertilizers has caused a series of problems such as environmental pollution, soil compaction, and ecological balance damage. With the deterioration of the environment, the problem of pesticide pollution has attracted more and more attention, so it is particularly important to find a method that is environmentally friendly and has a good control effect. [0003] Biological control has the advantages of less pollution, strong specificity, and less likely to produce drug resistance....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P1/00A01P3/00C05G3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C05G3/00C12N1/20C12N1/205C12R2001/07
Inventor 叶建仁丁森王焱张岳峰江明明陆蓝翔
Owner NANJING FORESTRY UNIV
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