Check patentability & draft patents in minutes with Patsnap Eureka AI!

Sensor based on affinity effect of boric acid, and preparation method and application thereof

A sensor, acid affinity technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problem of difficult removal of template molecules, and achieve the effect of low cost, stable performance and high promotion value

Inactive Publication Date: 2018-12-07
ANHUI NORMAL UNIV
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the preparation method of the sensor is simple, and the epinephrine template in the molecularly imprinted polymer can be removed by electrochemical elution, which solves the problem of difficult removal of template molecules and has high promotion value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sensor based on affinity effect of boric acid, and preparation method and application thereof
  • Sensor based on affinity effect of boric acid, and preparation method and application thereof
  • Sensor based on affinity effect of boric acid, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] The invention provides a method for preparing a sensor based on boric acid affinity, the method comprising: (1) mixing 3-thiophene boronic acid, epinephrine and a buffer solution to form a pre-polymerization solution; (2) mixing a glassy carbon electrode Immerse in the pre-polymerization solution, and use cyclic voltammetry to electropolymerize to obtain a polymer film-modified electrode; (3) immerse the polymer film-modified electrode in acid solution, and use cyclic voltammetry to scan repeatedly to elute adrenaline.

[0018] Through the above technical solution, the present invention introduces a new functional monomer 3-thiophene boronic acid (3-TBA), which is used to prepare molecularly imprinted polymer membranes, and expands the types of functional monomers. By electropolymerizing a layer of molecularly imprinted polymer film with 3-thiophene boronic acid as a new functional monomer on the glassy carbon electrode, combined with the molecular imprint left by adrena...

Embodiment 1

[0046] Add 5mL 4.0×10 to the clean electrolytic cell -3 mol / L HAuCl 4 solution, using the i-t method to perform electrochemical deposition of gold nanoparticles (AuNPs) on the polished glassy carbon electrode at -0.2V to obtain a gold nanoparticle-modified glassy carbon electrode (AuNPs / GCE);

[0047] Take 400uL 3-TBA (concentration: 0.05mol / L) and 100uL epinephrine (concentration: 0.05mol / L) into 5mL of PBS buffer solution (pH6.0), mix and stir to form a pre-polymerization solution;

[0048] The glassy carbon electrode modified by gold nanoparticles is immersed in the pre-polymerization solution, and electropolymerized by cyclic voltammetry (CV) in the potential range of -0.2V to 1.2V to obtain a polymer film modified electrode;

[0049] Submerge the polymer membrane modified electrode in hydrochloric acid (concentration: 0.05mol / L), and use the CV method to cycle scan 20 times in the potential range of 0-1.5V to elute the template molecule adrenaline, and obtain a boronic a...

Embodiment 2

[0051] The preparation method of the sensor based on boronic acid affinity:

[0052] (1) Mix 3-thiopheneboronic acid, epinephrine and phosphate buffer solution (pH 4, concentration 0.08mol / L) to form a pre-polymerization solution, wherein the molar ratio of 3-thiopheneboronic acid and epinephrine is 3:1 , the concentration of adrenaline in the pre-polymerization liquid is 0.8mmol / L;

[0053] (2) Immerse the glassy carbon electrode in the pre-polymerization solution, and use cyclic voltammetry electropolymerization (the potential range is -0.2V to 1.2V, and the number of polymerization cycles is: 30 cycles) to obtain a polymer film modified electrode;

[0054] (3) Immerse the polymer membrane-modified electrode in hydrochloric acid (the concentration of hydrogen ions is 0.03 mol / L), and use cyclic voltammetry (potential range 0-1.5 V) to scan 25 times to elute epinephrine.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Electric potentialaaaaaaaaaa
Login to View More

Abstract

The invention relates to an electrochemical sensor, and concretely discloses to a preparation method of a sensor based on the affinity of boric acid. The method comprises the following steps: (1) mixing 3-thienylboric acid, epinephrine and a buffer solution to form a prepolymerization solution; 2) immersing a glassy carbon electrode in the prepolymerization solution, and performing cyclic voltammetry electropolymerization to obtain a polymer film modified electrode; and (3) immersing the polymer film modified electrode in an acid solution, and performing cyclic scanning by cyclic voltammetry,and eluting adrenaline. The sensor can realize rapid and sensitive detection of adrenaline, and has the advantages of strong anti-interference ability, high sensitivity, simplicity in preparation, lowcost and stable performances.

Description

technical field [0001] The invention relates to an electrochemical sensor, in particular to a sensor based on boric acid affinity and its preparation method and application. Background technique [0002] Molecular recognition: The process of molecular recognition refers to the process in which molecules combine with each other through the synergy of intermolecular forces under specific conditions. Similar to the relationship between "key and lock", it vividly describes the specificity of the combination between molecules. So far, molecular recognition can be simplified as a process in which a subject selectively binds to an object and produces a specific function. Molecular recognition in organisms usually occurs between antibodies and antigens, enzymes and substrates, hormones and receptors. Based on this platform, technologies such as enzyme simulation, drug analysis and bionic sensing have been developed rapidly. [0003] The adrenal gland is an important endocrine org...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 阚显文刘芬
Owner ANHUI NORMAL UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com