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A kind of detection reagent card for Helicobacter pylori type detection and preparation method thereof

A technology of Helicobacter pylori and detection reagent, applied in the field of quantum dot immunofluorescence chromatography in medical immunology

Active Publication Date: 2021-08-13
重庆新赛亚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many detection methods, there are very few that are simple, fast and able to detect typing

Method used

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  • A kind of detection reagent card for Helicobacter pylori type detection and preparation method thereof
  • A kind of detection reagent card for Helicobacter pylori type detection and preparation method thereof
  • A kind of detection reagent card for Helicobacter pylori type detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, the preparation of detection reagent card

[0040] 1. Optimization of sample pad 1:

[0041] 1.1. Materials and reagents:

[0042] (1) Sample pad 1: made of glass fiber.

[0043] (2) Sample pad optimization buffer: 50mmol / L, tris of pH8.2, Tween 20 with a mass percentage concentration of 0.5%, proclin300 with a mass percentage concentration of 0.1%, and The balance of purified water.

[0044] 1.2. Immerse the sample pad 1 in the sample pad optimization buffer solution for 30-45 minutes, then dry it in the drying room for more than 5 hours, and control the humidity below 30%.

[0045] 2. Optimization of Quantum Mat 2:

[0046] 2.1. Materials and reagents

[0047] (1) Quantum pad 2: glass fiber material.

[0048] (2) Quantum pad optimization buffer: 50mmol / L, tris of pH8.2, bovine serum albumin (BSA) with a mass percentage concentration of 1%, and a mass percentage concentration of 0.5% Tween 20, 2% sucrose by mass, 0.1% proclin300 by mass, and purifi...

Embodiment 2

[0098] Embodiment 2, Helicobacter pylori type detection method

[0099] (1) Add the serum to be tested to the sample pad (1) of the detection reagent card in Example 1, and react at room temperature for 15 minutes.

[0100] (2) Insert the detection reagent card into the iSIA01 fluorescence immunoassay analyzer, and read the positive and negative of the antibody.

[0101] Detection method and detection result

[0102] (1) Use a large number of three types of normal negative sera to measure the concentration values. Since the reliability is greater than 99%, the mean value + 3SD measured by each serum is defined as the positive judgment value.

[0103] (2) The minimum detection limit of the detection reagent card of Example 1 of the present invention: the sensitivity reference products of Helicobacter pylori urease Urease antibody, cytotoxin-associated protein CagA antibody and vacuolar cytotoxin VacA antibody were respectively 1:3, 1: 9. 1:27 dilution, the reference substance...

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Abstract

The invention relates to a detection reagent card for the type detection of Helicobacter pylori, which comprises a sample pad, a quantum pad, a nitrocellulose membrane, a water-absorbing paper and a liner, and the quantum pad is coated with an antigen complex labeled with quantum dot microspheres, The antigen complex includes Urease antigen, CagA antigen, VacA antigen and mouse IgG; the first detection line, the second detection line, the third detection line and the quality control line are arranged on the nitrocellulose membrane, and the first detection line is coated with VacA antigen, the second detection line is coated with CagA antigen, the third detection line is coated with Urease antigen, and the quality control line is coated with goat anti-mouse IgG. The detection reagent card for detecting the typing of Helicobacter pylori provided by the present invention can obtain the result quickly and accurately, and can judge the infection type of Helicobacter pylori according to the detection result, and then provide auxiliary diagnosis for relevant clinical diseases.

Description

technical field [0001] The invention belongs to the technical field of quantum dot immunofluorescence chromatography in medical immunology, and in particular relates to a detection reagent card for typing detection of Helicobacter pylori and a preparation method thereof. Background technique [0002] Epidemiological studies have shown that the infection rate of Helicobacter pylori (HP) is very high, and about 50% of the world's people carry HP. pathogenic factor. The incidence rate is closely related to socioeconomic level, population density, public health conditions and water supply. Epidemiological studies have shown that HP infection mostly occurs in childhood, and in the absence of antibiotic treatment, HP infection usually accompanies lifelong; the genotype of strains infected by children is not changed by environmental migration, and is often consistent with the HP genotype of parental infection. In addition, HP infection is also the most widespread chronic bacteria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56916
Inventor 张念林赵亚丽孙婵马丹丹左云国
Owner 重庆新赛亚生物科技有限公司
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