Preparation method of double-layer artificial small-diameter blood vessel with modified inner layer
A small-diameter, inner-layer technology, applied in the manufacture of rayon, single-component polyurethane rayon, conjugated synthetic polymer rayon, etc. Compatibility and degradation performance, no inflammatory reaction, good blood compatibility
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Embodiment 1
[0028] A method for preparing a composite double-layer artificial small-caliber blood vessel with an inner layer modified by MSN-PEG-Heparin:
[0029] (1) Ultrasonic disperse 40 mg of carboxylated mesoporous silicon (MSNs-COOH) into 20 mL of 0.01N NaOH, stir for 10 minutes and centrifuge for 20 minutes, and wash the MSNs with water for 3 times; then, MSNs are dispersed in 10 mL of 0.05M In the mixed solution of MES and 0.5M NaCl, after stirring for 1 hour, 0.12M EDC, 2mg / mL PEG and 0.06M NHS were added. The mixture solution was stirred at room temperature for 16 hours. The final product was washed with water for 3 times and centrifuged to obtain MSN-PEG;
[0030] (2) Disperse 40 mg of the prepared MSN-PEG in 10 mL of 15% heparin solution and stir for 24 hours. MSN-PEG-Heparin was obtained after centrifugation;
[0031] (3) Disperse the MSN-PEG-Heparin prepared in (2) in HFIP (3mg / mL), then sonicate for 30 minutes to ensure uniform dispersion;
[0032] (4) Prepare the inner...
Embodiment 2
[0036] A method for preparing a composite double-layer artificial small-caliber blood vessel with an inner layer modified by MSN-PEG-Heparin:
[0037] (1) Ultrasonic disperse 40 mg of carboxylated mesoporous silicon (MSNs-COOH) into 20 mL of 0.01N NaOH, stir for 10 minutes and centrifuge for 20 minutes, and wash the MSNs with water for 3 times; then, MSNs are dispersed in 10 mL of 0.05M In the mixed solution of MES and 0.5M NaCl, after stirring for 1 hour, 0.12M EDC, 2mg / mL PEG and 0.06M NHS were added. The mixture solution was stirred at room temperature for 16 hours. The final product was washed with water for 3 times and centrifuged to obtain MSN-PEG;
[0038] (2) Disperse 40 mg of the prepared MSN-PEG in 10 mL of 15% heparin solution and stir for 24 hours. MSN-PEG-Heparin was obtained after centrifugation;
[0039](3) Disperse the MSN-PEG-Heparin prepared in (2) in HFIP (3mg / mL), then sonicate for 30 minutes to ensure uniform dispersion;
[0040] (4) Prepare the inner ...
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