Method for extracting cynaroside and quercetin-3-o-beta-glucopyranoside from leaves of populus tomentosa

A technology of glucopyranoside and extraction method, which is applied in the field of extraction of luteolin and quercetin-3-o-β-glucopyranoside, which can solve the problems of large environmental pollution and large solvent consumption

Active Publication Date: 2018-12-14
NINGXIA MEDICAL UNIV
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Problems solved by technology

At present, the extraction methods of luteolin and quercetin-3-o-β-glucopyranoside mostly have the disadvantages of large solvent consumption and large environmental pollution. The Chinese invention patent of "method for preparing

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  • Method for extracting cynaroside and quercetin-3-o-beta-glucopyranoside from leaves of populus tomentosa
  • Method for extracting cynaroside and quercetin-3-o-beta-glucopyranoside from leaves of populus tomentosa
  • Method for extracting cynaroside and quercetin-3-o-beta-glucopyranoside from leaves of populus tomentosa

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Embodiment Construction

[0015] The present invention will be described in detail below with reference to the specific embodiments, so that those skilled in the art can better understand the advantages of the present invention.

[0016] A method for extracting luteolin and quercetin-3-o-β-glucopyranoside in poplar tomentosa leaves, comprising:

[0017] Step S1, pulverize 20g of poplar tomentosa leaves, use an ethanol solution with a concentration of 50 to 90%, and the consumption ratio of the leaves to the ethanol solution is 1g: 3mL to 12mL, then heated to 70 ℃ and kept at a constant temperature, and refluxed for extraction. The time of the first reflux extraction is 1-2.5h. After 1-4 reflux extractions, the extracts are combined and dried by rotary evaporation to a fluid extract equivalent to 0.9-1.1 g of the crude drug per 1 mL of concentrated solution, and the evaporation temperature is 50 ℃. The crude product is obtained, and refrigerated for later use, wherein, the consumption ratio of the leave...

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Abstract

The invention discloses a method for extracting cynaroside and quercetin-3-o-beta-glucopyranoside from leaves of populus tomentosa, which comprises the following steps: the leaves of the populus tomentosa are heated after crushed and added with ethanol solution, reflux extraction is carried repeatedly, and obtained extracted solutions are mixed; the extracted solutions are added with macroporous adsorbent resin and put into chromatographic columns, absorption is carried out after flushing, eluents are respectively collected after elution is carried out repeatedly, concentration and drying arecarried after pressure is reduced, and 40% ethanol elution extracts which are maximum in total flavonoid content are mixed for obtaining a primary extraction material; the primary extraction materialis added with polyamide resin and put into the chromatographic columns, absorption is carried out after flushing, the eluents are respectively collected after elution is carried out repeatedly, concentration and drying are carried after pressure is reduced, and 15 mg of 20% ethanol elution extracts which are maximum in total flavonoid content are further purified for obtaining a secondary extraction material; and the secondary extraction material is added with octadecylsilyl (ODS) and put in the chromatographic columns, absorption is carried out after flushing, the eluents are respectively collected after elution is carried out repeatedly, and concentration and drying are carried after pressure is reduced for obtaining the cynaroside and the quercetin-3-o-beta-glucopyranoside.

Description

technical field [0001] The invention relates to the technical field of extraction methods of luteolin and quercetin-3-o-β-glucopyranoside, in particular to a kind of luteolin and quercetin-3-o-β-pyridine in the leaves of poplar tomentosa Extraction method of glucopyranoside. Background technique [0002] Poplar tomentosa leaf is a kind of folk herbal medicine, which is used in folk and clinical treatment for osteomyelitis with severe bedsores, and the curative effect is remarkable. Populus tomentosa is the bark or twig of Populus tomentosa, a plant belonging to the family Salix family. Modern research has found that Populus plants have antibacterial, anti-inflammatory, antiviral, analgesic, cardiovascular protection, cytotoxic and other activities. Luteolin is a flavonoid glycoside compound existing in natural plants, which has expectorant and antitussive effects, and also has the effect of lowering cholesterol in atherosclerosis and enhancing capillary relaxation. It als...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 付雪艳郭东燕董琳李娟黄俊张新慧吴秀丽
Owner NINGXIA MEDICAL UNIV
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