Method for preparing three-dimensional mineralized collagen scaffold material with hierarchical structure

A technology for mineralizing collagen and scaffolding materials, applied in the field of materials, can solve problems such as unexplainable hierarchical structure

Inactive Publication Date: 2018-12-18
杭州恒睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these theories are only applicable to explain the intrafibril nucleation of hydroxyapatite and cannot explain the generation of hierarchical structure

Method used

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  • Method for preparing three-dimensional mineralized collagen scaffold material with hierarchical structure
  • Method for preparing three-dimensional mineralized collagen scaffold material with hierarchical structure
  • Method for preparing three-dimensional mineralized collagen scaffold material with hierarchical structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation of a three-dimensional mineralized collagen scaffold material with a multi-level structure, including the following raw materials and procurement sources: rat tail type I procollagen solution (BD Bio-sciences), dialysis bag (3500Da, Invitrogen, Paisly, UK), PAA ( M.W.72, 2000, 5000) (Sigma-Aldrich, USA), phosphate buffer (Gibco, Invitrogen, Paisly, UK) and glycine (Sigma-Aldrich, USA).

[0036] The method for preparing a three-dimensional mineralized collagen scaffold material with a multi-level structure; the specific steps are as follows:

[0037] (1) Configuration of phosphate buffer: weigh 8g of NaCl, 0.2g of KCl, 3g of Na2HPO4, 0.29g of Ka 2 HPO 4 ·3H 2 O and 0.2g of Na3N were dissolved in 1L of deionized water, and the pH of the solution was adjusted to 7.4 with NaOH and HCl;

[0038] (2) Collagen mineralization: The dialysis bag (molecular weight cut off: 3500) filled with 10.6 mL of 9.46 mg / mL rat tail type I procollagen solution was immersed in...

Embodiment 2

[0042] A preparation of a three-dimensional mineralized collagen scaffold material with a multi-level structure, including the following raw materials and procurement sources: rat tail type I procollagen solution (BD Bio-sciences), dialysis bag (3500Da, Invitrogen, Paisly, UK), PAA ( M.W.72, 2000, 5000) (Sigma-Aldrich, USA), phosphate buffer (Gibco, Invitrogen, Paisly, UK) and glycine (Sigma-Aldrich, USA).

[0043] The method for preparing a three-dimensional mineralized collagen scaffold material with a multi-level structure; the specific steps are as follows:

[0044] (1) Configuration of phosphate buffer: weigh 8g of NaCl, 0.2g of KCl, 3g of Na2HPO4, 0.29g of Ka 2 HPO 4 ·3H 2 O and 0.2g of Na3N were dissolved in 1L of deionized water, and the pH of the solution was adjusted to 7.4 with NaOH and HCl;

[0045] (2) Collagen mineralization: The dialysis bag (molecular weight cut off: 3500) filled with 10.6 mL of 9.46 mg / mL rat tail type I procollagen solution was immersed in...

Embodiment 3

[0049] A preparation of a three-dimensional mineralized collagen scaffold material with a multi-level structure, including the following raw materials and procurement sources: rat tail type I procollagen solution (BD Bio-sciences), dialysis bag (3500Da, Invitrogen, Paisly, UK), PAA ( M.W.72, 2000, 5000) (Sigma-Aldrich, USA), phosphate buffer (Gibco, Invitrogen, Paisly, UK) and glycine (Sigma-Aldrich, USA).

[0050] The method for preparing a three-dimensional mineralized collagen scaffold material with a multi-level structure; the specific steps are as follows:

[0051] (1) Configuration of phosphate buffer: Weigh 8g of NaCl, 0.2g of KCl, 3g of Na2HPO4, 0.29g of Ka2HPO4 3H2O, 0.2g of Na3N and dissolve them in 1L of deionized water, adjust the pH of the solution with NaOH and HCl in 7.4;

[0052] (2) Collagen mineralization: The dialysis bag (molecular weight cut off: 3500) filled with 10.6 mL of 9.46 mg / mL rat tail type I procollagen solution was immersed in 30 mL of phosphat...

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Abstract

The invention discloses a method for preparing a three-dimensional mineralized collagen scaffold material with a hierarchical structure. Calcium ions are complexed and transferred by controlling the free energy of an intermediate and selecting a polymer with a suitable molecular weight and a suitable concentration to prepare the three-dimensional mineralized collagen scaffold material with the bone-like hierarchical structure, a non-hierarchical structure collagen scaffold and an extrafibrillar mineralized collagen scaffold material. Polyacrylic acid and the calcium ions form the intermediateto release calcium ions and promote nucleation crystallization of hydroxyapatite on collagen fibers, the energy of the intermediate is too high, and the hydroxyapatite is deposited in the gap area andthe overlapped area of the collagen fibers indistinctively and is nucleated to cause the formation of the non-hierarchical structure collagen scaffold and make the energy of the intermediate too low,so the fibrotic mineralized collagen scaffold is generated to realize the perfect simulation of natural bones, and the method has wide application values in the field of production of bone repairingmaterials.

Description

technical field [0001] The invention relates to the field of materials, in particular to a method for preparing a three-dimensional mineralized collagen scaffold material with a multi-level structure. Background technique [0002] Bone is a complex, dynamic substance. Bone contains a mineralized matrix with a central cavity containing red marrow that ensures the integrity of bone tissue in mammals at all times. In clinical practice, maxillofacial bone tissue defects due to tumors, trauma, and genetic diseases have remained high. According to statistics, there are millions of people with bone defects caused by tumor resection every year. With the insufficient supply of organs and tissues, the rapid development of tissue engineering using natural or synthetic biomaterial grafts such as polymers, metals, ceramics, autologous or allograft bone as grafts has emerged. To prepare natural bone-like materials, it is necessary to completely replicate its hierarchical structure and ...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/16A61L27/12A61L27/50A61L27/56
CPCA61L27/12A61L27/16A61L27/24A61L27/50A61L27/56A61L2430/02C08L33/02
Inventor 刘燕周彦恒罗聃
Owner 杭州恒睿生物科技有限公司
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