Method for extracting Kadsura coccinea fruit polysaccharides

Abstract

The invention discloses a method for extracting Kadsura coccinea fruit polysaccharides. The method comprises the following steps: 1, taking Kadsura coccinea fruits, and sequentially performing cleaning, seed removal, drying, crushing and sieving to obtain fruit powder; 2, taking the fruit powder and an ethanol-water solution, performing reflux stirring, carrying out filtration to obtain filter residues, and repeating above steps three times; 3, taking the obtained filter residues, mixing the filter residues with distilled water, performing reflux stirring, concentrating the obtained solution,adding anhydrous ethanol, performing cold storage, performing centrifugation, taking the obtained lower precipitate, sequentially washing the precipitate with anhydrous ethanol, acetone and ether, andairing the washed precipitate to obtain crude polysaccharides; and 4, taking the crude polysaccharides, carrying out protein removal treatment on the crude polysaccharides, and performing vacuum freeze drying to obtain the Kadsura coccinea fruit polysaccharides. The method is simple and feasible, and the Kadsura coccinea fruit polysaccharides have a high extraction rate and a good anti-oxidationactivity.

Description

technical field [0001] The invention relates to the technical field of plant extraction. More specifically, the present invention relates to a method for extracting black tiger fruit polysaccharide. Background technique [0002] Black tiger is an evergreen woody vine wild plant of the genus Schisandra in the family Magnoliaceae. The fruit of black tiger is an aggregate fruit, also known as Lengfantuan, Bufuna, etc. It is a rare folk Chinese herbal medicine. The medicinal parts include roots, stems and fruits. In modern medical research, pharmacological experiments such as Liu Xijun proved that black tiger root has a certain sedative and anti-inflammatory effect, Feng Yi et al. found that the root of black tiger has antibacterial effect on pneumococcus, and the antibacterial effect of pericarp on Salmonella typhi, Goh MJ et al. It shows that the black tiger extract can inhibit the synthesis of melanin. Wang Laiyou and others found that the black tiger extract can regulate al...

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Problems solved by technology

In modern medical research, pharmacological experiments such as Liu Xijun proved that black tiger root has a certain sedative and anti-inflammatory effect, Feng Yi et al. found that the root of black tiger has antibacterial effect on pneumococcus, and the antibacterial effect of pericarp on Salmonella typhi, Goh MJ et al. It shows that the black tiger extract can inhibit the synthesis of melanin. Wang Laiyou and others found that the black tiger extract can regulate alcoholic fatty liver and protect the liver. Peng Mijun and others found that the pulp is rich in amino acids and trace elements. Sun Jian et al. It has been shown that it has antioxidant activity, but there are few reports on the extraction method and antioxidant activity of black tiger fruit polysaccharides