Method for constructing high temperature stress-resistant recombinant Mycobacterium smegmatis

A kind of technology of Mycobacterium smegmatis and construction method, applied in the field of construction of recombinant Mycobacterium smegmatis
CN109022337APending Publication Date: 2018-12-18FOSHAN UNIVERSITY
5 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FOSHAN UNIVERSITY
Publication Date
2018-12-18

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for constructing high temperature stress-resistant recombinant Mycobacterium smegmatis. The method is characterized in that an HtpG gene fragment is introduced to makethe recombinant Mycobacterium smegmatis have the function of expressing an HtpG protein. The HtpG gene fragment is introduced to Mycobacterium smegmatis to obtain the high temperature stress-resistant recombinant Mycobacterium smegmatis, so a problem that an hsp60 promoter in the Mycobacterium smegmatis expression system needs a high temperature to increase the expression activity and the high temperature is not conducive to the growth of the Mycobacterium smegmatis is solved. The method has great reference values in the biology research of mycobacteria for expressing specific genes with theMycobacterium smegmatis as a host strain and the development of new anti-tuberculosis vaccinse and microbial reactors. The invention also discloses the high temperature stress-resistant recombinant Mycobacterium smegmatis obtained by the construction method, and a primer pair for the PCR amplification of the Mycobacterium tuberculosis HtpG gene fragment.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for constructing a recombinant mycobacterium smegmatis resistant to high temperature stress. Background technique

[0002] Mycobacterium smegmatis was first reported by Lustgarten in 1884, and then Alvarez and Tavel also found the bacteria in genital secretions (smegma, smegma), so it was named mycobacterium smegmatis (mycobacterium smegmatis). Mycobacterium smegmatis belongs to the genus Actinomycetes and is also commonly found in soil, marine and freshwater environments.

[0003] Mycobacterium smegmatis is a non-pathogenic microorganism that shares more than 2000 homologous genes with Mycobacterium tuberculosis, the pathogenic bacterium that causes tuberculosis, and has the same cell wall structure as Mycobacterium tuberculosis and other mycobacteria. M. smegmatis is readily cultured in most synthetic or complex laboratory media, forming visible colonies in 3 to 5 days. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More