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Method for simultaneously measuring contents of 7 polyphenol active ingredients in different varieties of osmanthus fragrans through HPLC-MS-MS method

A HPLC-MS-MS, active ingredient technology, used in measurement devices, material separation, analysis of materials, etc.

Pending Publication Date: 2018-12-18
HUBEI UNIV OF SCI & TECH
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Problems solved by technology

At present, the determination of the content of sweet-scented osmanthus is mainly carried out by ultraviolet spectrophotometry and high-performance liquid chromatography, and there are no domestic and foreign literature reports on the analysis of polyphenols active ingredients in sweet-scented osmanthus by liquid chromatography-mass chromatography

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  • Method for simultaneously measuring contents of 7 polyphenol active ingredients in different varieties of osmanthus fragrans through HPLC-MS-MS method
  • Method for simultaneously measuring contents of 7 polyphenol active ingredients in different varieties of osmanthus fragrans through HPLC-MS-MS method
  • Method for simultaneously measuring contents of 7 polyphenol active ingredients in different varieties of osmanthus fragrans through HPLC-MS-MS method

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Embodiment Construction

[0041] The following are specific embodiments of the present invention and in conjunction with the accompanying drawings, the technical solutions of the present invention are further described, but the present invention is not limited to these embodiments.

[0042] 1 Materials, reagents and instruments

[0043] 1.1 Materials: Osmanthus fragrans samples (Osmanthus fragrans Town, Xianning City, Hubei Province, harvested in September 2017), dried at 60°C to constant weight, crushed through a 0.25mm sieve for later use. It was identified as the dried petals of Osmanthus fragrans Lour. by Professor Chen Hongguo from the School of Nuclear Technology and Chemical Biology of Hubei University of Science and Technology.

[0044] 1.2 Reagent: Methanol is a chromatographically pure reagent (Sigema Company); formic acid (Deoksan Pharmaceutical Industry, South Korea); purified water (Hangzhou Wahaha Group Co., Ltd.); reference substance (purity ≥ 98%): chlorogenic acid, caffeic acid, Actasco...

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Abstract

The invention provides a method for simultaneously measuring the contents of 7 polyphenol active ingredients in different varieties of osmanthus fragrans through an HPLC-MS-MS method, and belongs to the field of biochemical technology. The method comprises the following steps: performing gradient elution by using a chromatographic column Shim-pack VP-ODS (2.0 x 150mm, 5[mu]m) and using methanol-0.1% formic acid water as a mobile phase, wherein the volume flow rate is 0.2mL.min-1, the column temperature is 30 DEG C, and the sample size is 5 uL; and using an ESI ion source as a mass spectrum, and performing detection in a multi-reaction monitoring anion scanning method, wherein the result indicates that the 7 components have a good linear relationship within a measured concentration range. The precision, the repeatability and the stability are good; and the requirements of methodological investigation are met. The established method is simple, rapid, sensitive and highly specific, and can be used for the simultaneous measurement of 7 polyphenol active ingredients in the osmanthus fragrans, namely, chlorogenic acid, caffeic acid, verbascoside, naringin, rutin, ferulic acid and quercetin.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a method for simultaneously measuring the contents of seven polyphenol active components in different varieties of sweet-scented osmanthus by means of an HPLC-MS-MS method. Background technique [0002] Phenolic compounds are a class of major secondary metabolites originating in plants and are natural phytochemicals. Mainly from phenylalanine, less from tyrosine, widely present in food and nutritional products. Phenolic compounds vary in structure, but can be defined as having at least one hydroxyl group with one or more aromatic rings. According to the number of hydroxylated aromatic rings and the type of functional moiety, the main polyphenols are: flavonoids, phenolic acids, phenolic alcohols, stilbenes and lignans. Polyphenols are an important part of the human diet and are widely found in berries, grapes / wine, tea, chocolate / cocoa, coffee, soybeans and other fruits and ...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈洪国宋晓芳
Owner HUBEI UNIV OF SCI & TECH
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