Heterotrophic nitrification-aerobic denitrification compound microorganism preparation and preparation method thereof
A composite microorganism and aerobic denitrification technology, which is applied in the field of heterotrophic nitrification-aerobic denitrification composite microbial preparation and its preparation, can solve the problems of unfavorable and rapid control of ammonia nitrogen content in water, many process links and high treatment costs, and achieves pH The effect of stable value, cost reduction and high ammonia nitrogen removal rate
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Embodiment 1
[0039] 1. Mix Vibrio alginolyticus and Pseudomonas aeruginosa in a ratio of 1:2.31;
[0040] 2. The medium composition is:
[0041] The medium formula is (g / L): Tryptone 10; Yeast extract 5; NaCl 10; NH 4 Cl 0.5; sodium citrate 5.66; 50 mL of Vickers salt solution, seawater (NaCl 15) with a salinity of 15‰, adjust the pH to 7.0.
[0042] Vickers salt solution (g / L): K 2 HPO 4 5.0; MgSO 4 ·7H 2 O 2.5; FeSO 4 ·7H 2 O 0.05; MnSO 4 ·4H 2 O0.05.
[0043] 3. Inoculate the mixture of step 1 at 5% of the volume of the medium, and cultivate it at a temperature of 25 degrees Celsius and a rotation speed of 100 to 130 revolutions per minute, with a pH of 7.0 to 7.5;
[0044] 4. Detect the OD value of the culture after culturing for 20 hours. When the OD value reaches 2.15±0.02, the fermentation is completed.
Embodiment 2
[0046] 1. Mix Vibrio alginolyticus and Pseudomonas aeruginosa in a ratio of 1:5.52;
[0047] 2. The medium composition is:
[0048] The medium formula is (g / L): Tryptone 10; Yeast extract 5; NaCl 10; NH 4 Cl 0.5; sodium citrate 5.66; 50 mL of Vickers salt solution, seawater (NaCl 15) with a salinity of 15‰, adjust the pH to 7.0.
[0049] Vickers salt solution (g / L): K 2 HPO 4 5.0; MgSO 4 ·7H 2 O 2.5; FeSO 4 ·7H 2 O 0.05; MnSO 4 ·4H 2 O0.05.
[0050] 3. Inoculate the mixture of step 1 at 1% of the volume of the culture medium, and cultivate it at a temperature of 35 degrees Celsius and a rotation speed of 100 to 130 revolutions per minute, with a pH of 7.0 to 7.5;
[0051] 4. Detect the OD value of the culture after 30 hours of incubation. When the OD value reaches 2.15±0.02, the fermentation is completed.
Embodiment 3
[0053] 1. Mix Vibrio alginolyticus and Pseudomonas aeruginosa in a ratio of 1:3;
[0054] 2. The medium composition is:
[0055] The medium formula is (g / L): Tryptone 10; Yeast extract 5; NaCl 10; NH 4 Cl 0.5; sodium citrate 5.66; 50 mL of Vickers salt solution, seawater (NaCl 15) with a salinity of 15‰, adjust the pH to 7.0.
[0056] Vickers salt solution (g / L): K 2 HPO 4 5.0; MgSO 4 ·7H 2 O 2.5; FeSO 4 ·7H 2 O 0.05; MnSO 4 ·4H 2 O0.05.
[0057] 3. Inoculate the mixture of step 1 at 3% of the volume of the medium, and cultivate it at a temperature of 30 degrees Celsius and a rotation speed of 100 to 130 revolutions per minute, with a pH of 7.0 to 7.5;
[0058] 4. Detect the OD value of the culture after 30 hours of incubation. When the OD value reaches 2.15±0.02, the fermentation is completed.
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